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二甲基联苯胺

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Medical grade SiO was injected into cat eyes after gas-compression vitrectomy. Balanced salt solution was injected into the other eyes as a control. All cats were examined with biomicroscopy and ophthalmoscope pre and postoperatively; intraocular pressure was also measured regularly. After 1, 3 or 6 months of cat's survival, the morphology of RGCs labeled with retrograded HRP injected into the bilateral lateral geniculate nucleus and reacted with diaminobenzidine was quantitatively analyzed.

30只健康成年家猫,气体玻璃体切割后实验眼注入医用硅油,对照眼注入平衡盐溶液,术后定期眼部检查并监测眼压。1、3、6月后进行形态学实验:(1)微电极定位双侧外侧膝状体并注入辣根过氧化物酶,48~60小时后灌注固定,剥取视网膜行二甲基联苯胺成色反应;(2)视网膜尼氏染色;(3)眼球矢状切片常规HE染色。

The optimum condition of the ELISA are set as follows: 1ug/mL of NFLX -HSA(norfloxacin -human serum albumin) conjugation used as a coated antigen dissolved in carbonate buffer solution(CBS,pH 9.6,0.05mol/L), 0.5 % glutin in PBS(pH7.4,0.01mol/L) as a blocking agent post-coating, horse radish heroxidase-labeled sheep anti-mouse IgG(HRP-anti IgG) diluted by PBS(pH 7.4,0.1mol/L) in 1:1000 as a secondary antibody, and TMB (3,3",5,5"-tetramethyl benzidine dihydrochloride) as a sbustrate.

成功建立了检测抗诺氟沙星抗体的间接ELISA方法,其主要条件如下:pH9.6、0.005mol/L的碳酸盐缓冲溶液为包被液,1μg/mL的连接抗原NFLX-BSA(诺氟沙星-牛血清白蛋白)为包被抗原,1%明胶为封闭液,1:1000稀释的HRP酶标记的羊抗小鼠抗体作为二抗,TMB(3,3,5,5-四甲基联苯胺)作为底物。

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Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.

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