二甲基联苯胺

Medical grade SiO was injected into cat eyes after gas-compression vitrectomy. Balanced salt solution was injected into the other eyes as a control. All cats were examined with biomicroscopy and ophthalmoscope pre and postoperatively; intraocular pressure was also measured regularly. After 1, 3 or 6 months of cat's survival, the morphology of RGCs labeled with retrograded HRP injected into the bilateral lateral geniculate nucleus and reacted with diaminobenzidine was quantitatively analyzed.

30只健康成年家猫，气体玻璃体切割后实验眼注入医用硅油，对照眼注入平衡盐溶液，术后定期眼部检查并监测眼压。1、3、6月后进行形态学实验：（1）微电极定位双侧外侧膝状体并注入辣根过氧化物酶，48～60小时后灌注固定，剥取视网膜行二甲基联苯胺成色反应；（2）视网膜尼氏染色；（3）眼球矢状切片常规HE染色。

The optimum condition of the ELISA are set as follows: 1ug/mL of NFLX -HSA(norfloxacin -human serum albumin) conjugation used as a coated antigen dissolved in carbonate buffer solution(CBS,pH 9.6,0.05mol/L), 0.5 % glutin in PBS(pH7.4,0.01mol/L) as a blocking agent post-coating, horse radish heroxidase-labeled sheep anti-mouse IgG(HRP-anti IgG) diluted by PBS(pH 7.4,0.1mol/L) in 1:1000 as a secondary antibody, and TMB (3,3",5,5"-tetramethyl benzidine dihydrochloride) as a sbustrate.

成功建立了检测抗诺氟沙星抗体的间接ELISA方法，其主要条件如下：pH9.6、0.005mol／L的碳酸盐缓冲溶液为包被液，1μg／mL的连接抗原NFLX-BSA(诺氟沙星-牛血清白蛋白)为包被抗原，1％明胶为封闭液，1：1000稀释的HRP酶标记的羊抗小鼠抗体作为二抗，TMB(3，3，5，5-四甲基联苯胺)作为底物。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。