二层的

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

The research of reservoir characteristics indicate that the T_1j~2_2 reservoir's pore spaces were dominated by intercrystalline pores and corrosion pores, there have 1 ~2 high porosity section by corrasion in Mainan structure, the porosity is 6~16%,and in this area reservoirs mainly belong to type Ⅱ and typeⅢ, the reservoir type was defined as fracture-pore type. and the reservoir physical property were primarily dominated by sedimentary fades、 corrosion and dolomization.

储层特征研究表明，嘉二~2储层以白云岩的晶间孔、溶孔为主，麦南构造存在1～2个溶孔发育的高孔段，孔隙度约为6～16%，储层主要为Ⅱ、Ⅲ类，储集类型属裂缝—孔隙型，储层物性主要受沉积微相、溶蚀作用及白云岩化作用的控制。

The device is fabricated by nonsilicon surface micro-machining, which uses the photoresist and the sputtered copper thin films as the scarificial layers, and the electroplated ferronickel as the structure layer to fabricate the optical MEMS components. The device consists of an electroplated ferronickel optical shutter interposed in a gap between two fiber alignment componets, an electroplated planar coil, a silicon spring, and two fiber alignment components with V-shaped grooves.

该衰减器利用非硅表面微加工工艺(这种工艺采用光致抗蚀剂和溅射铜薄膜作为牺牲层，电镀铁镍层作为结构层来制作MEMS元件)制作，由电镀铁镍层构成的一个插在两对准光纤空隙中的挡光片，一个平面电感线圈，一个硅弹簧和二个带有V型槽的光纤对准元件构成。

A Intercalary behavior of different molecular weight of PEG such as 2 000, 4 000 and 6 000 in VXG was studied. The intercalation of polymer increases the layer distance of host and occurs cooperative effect with it.

研究比较了2 000、4 000、6 000三种不同分子量的水溶性聚合物聚乙二醇在五氧化二钒干凝胶层状氧化物中的嵌入，PEG的嵌入增加了主体层状物的层间距并与主体材料发生协同作用。

Secondary pollution might be produced in the weak zone while conventional acidify because of halfway flowback under poor producing energy. Thief zones can obtain superfluous acidizing fluid because of the permeability contrast between layers of prominent interlamination antinomy, which can induce collapse in the thief zone and invalid acidification in the less permeable layers.

低压油气层常规酸化时由于地层能量低，导致残酸溶液无法彻底返排而引起地层的二次污染；层间矛盾突出的地层，由于渗透率差异，酸液将优先进入高渗地层，导致高渗透层进酸量多，从而过度酸化引起坍塌，低渗透层则由于进酸量少而达不到酸化的目的。

And then the deposition of CaCO3 took place on the multilayers by dipping them into a supersaturated CaCO3 solution.

将聚苯乙烯磺酸钠与聚二烯二甲基氯化铵用逐层浸渍的方法使其组装成多层膜，用于诱导过饱和溶液中CaCO3的结晶，详细研究了膜紫外吸收随组装层数增加的线性变化。

According to the J_2~2～J~1 strati- graphic features, rock facies characteristics and previous research, the author deem that the terrestrial environment spread all over the epicontinental sea-platform at the transgression time when the J_1 depositing, the environment character is the normal sea-platform sedimentary; the study results indicate that the location-evaporation platform environment when theJ_2~～J~1 sediment is good to the reservoir formatting and evaluating.

研究认为在嘉二~1～嘉二~2段时期沉积所处的局限—蒸发台地环境属于较有利于储层形成与演化的沉积相带；嘉一段沉积相平面展布主要形成于开阔台地环境中，属于不利于储层形成与演化的沉积相带。

At last, using the dual-porosity model, which is provided by LOGES logging interpretation system, two parameters of gas saturation and irreducible water saturation are used to help identify the doubtful pays with low resistance.

首先校正测井仪器或测井环境对曲线质量的影响，在此基础上进行标准化处理；然后通过&四性&关系研究，考虑到涩北气田储层欠压实等影响，采用补偿密度—电阻率交会的方法来编制气层解释图版，先对曲线进行一次解释，将易于识别的测井响应特征明显的气层剔除出来，然后对低阻可疑气层进行二次定量识别；最后，借助于LOGES测井解释系统提出的双孔隙度模型，利用可动水饱和度和地层水含气饱和度相结合建立新的测井解释标准，进而准确识别低阻可疑气层。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。