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Objective According to principle of forming primer dimer,a new cloning gene method was established.

目的根据引物二聚体形成原理,建立一种新的克隆目的基因的方法。

Parameter mode was to interpret the inheritance of the characters studied and the epistasis was significant.

数量遗传分析表明,角果长、千粒重的遗传符合加性显性二基因互作模型,存在显著的上位性效应。

The expression of LbMT2 gene in L. bicolor in response to CuSO4, CdCl2, NaCl, cold, and PEG was further investigated using real time quantitative PCR.

利用实时定量PCR方法研究了二色补血草在CuSO4、CdCl2、NaCl、低温和PEG胁迫下不同时间该基因的表达模式。

In this study, nine aspects had been carried out, which included the condition optimization of inducing silkworm asexual reproduction; the effective technique of obtaining unfertilized eggs from virginal moth; the investigation of asexual reproductive genetic resources on bivoltine silkworm races; the creating of bivoltine female asexual reproductive lines; the genetic effect analysis of silkworm asexual reproductive character; the eggs protein study on different female lines; growing and developing comparison between asexual reproductive line and its sexual parent; RAPD study on asexual reproductive character; the utilization of asexual reproductive lines.

本论文主要研究内容包括:家蚕无性繁殖诱导条件的优化;从家蚕处女蛾获取未受精卵的实用技术研究;二化性家蚕品种无性繁殖特性的遗传资源调查;二化性雌蚕无性繁殖系的构建;家蚕无性繁殖性状的遗传效应分析;家蚕无性繁殖品种的蛋白质研究;家蚕无性繁殖品种与其两性亲本生长发育对比研究;控制家蚕无性繁殖性状的基因的RAPD分子标记探索以及家蚕无性繁殖系的利用研究等九个方面,取得的主要结果如下

The hNaDCS (human Na+/dicarboxylate cotransporter 3) mediates the transport of Krebs cycle intermediates, such as succinate, citrate and a-ketoglutarate, across the plasma membrane of many epithelial cells.

摘要人钠/二羧酸协同转运蛋白3(human Na~+/dicarboxylate cotransporter3,hNaDC3)基因的编码产物是位于多种上皮细胞膜表面的一类介导琥珀酸、枸橼酸及α-酮戊二酸等三羧酸循环中间产物转运的蛋白。

Objective Applying randomized terminal linker-dependent PCR to detect the DNA damage of ras gene induced by dichloroacetic acid.

目的研究二氯乙酸(dichloroacetic acid,DCA)对ras基因的DNA损伤作用,探讨其致癌作用分子机制。

Lines which were 2n=21"+1" and one line which was 2n=20"+2", one line which was 2n=43. The salt-resistance addition line Line15 was confirmed as a Disomic alien addition line by C-banding, SSR, FISH methods, and the two alien addition chromosomes had close correlation with the long arm of the second homoeologous group, the centromere region of the third homoeologous group and the short arm of the seventh homoeologous group. We also confirmed the particular C-banding type of the alien addition chromosomes by C-banding following FISH method. The drought-resistance addition line Line17 was found also as a Disomic alien addition line by SSR and FISH detection. The alien addition chromosomes had close correlation with the short arm of the second homoeologous group.

对导入赖草优质耐盐基因的株系Line15进行了C-分带、荧光原位杂交、SSR相结合的鉴定,确认Line15是一个二体异附加系,附加的一对染色体与小麦的第二同源群长臂、第三同源群着丝粒和第七同源群短臂密切相关,并确定了Line15的外源染色体C-分带特征带型,为目后鉴定Line15附加系的真伪提供了简便快捷的途径;对抗旱附加系Line17进行了荧光原位杂交和SSR检测,得出Line17也是一个二体异附加系,外源染色体与小麦的第四同源群短臂密切相关。

A strain producing 2-oxoaldehyde reductase was isolated from LB culture medium containing methylglyoxal. By analyzing its physiological and biochemical characters, and 16S rDNA sequence, it was identified as S. maltophilia and named as Stenotrophomonas inaltophilia YL.

用含甲基乙二醛的LB培养基平板筛选到1株能代谢甲基乙二醛的细菌,通过对克隆到的16S rDNA基因序列比对及生理生化实验,将其鉴定为嗜麦芽寡养单胞菌,命名为Stenotrophomonas maltophilia YL。

Erve growth factor combined with its receptor, activated Ras - MAP kinase , mitogen - activated protein kinase kinase is the important regulating factor that make IkB kinase, IKK, phosphated, IkB kinase make the IκBα:(the subunit of NF -κB ) phosphated, the phosphated IkBα degraded, and p65 - p50 heterodimer can be formed, then the heterodimer translocated to nucleus and combined with the promoter domain or other consensus sequence.

GF与其受体相结合,最后可以激活Ras-有丝分裂激活的蛋白激酶途径,有丝分裂激活的蛋白酶激酶(MEKK1)是IγB激酶发生磷酸化的重要调节因子,IKK使NF-κB的亚单位IKBα发生磷酸化,磷酸化的IKBα发生降解,而最后留下p65-p50二聚体,该二聚体然后转位到细胞核内与κB基因的增强子区域或与他的顺式作用序列结合。

RESULTS: FISH demonstrated that the male pronuclei in some one-cell embryos and the each nucleus in some two-cell embryos presented positive signals.

结果: 在单细胞胚和二细胞胚间期核内发现HBV DNA阳性杂交信号,RT-PCR可以扩增出HBx基因的特异条带,免疫荧光也可见到HBsAg阳性信号。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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