二基因的

By splicing in genes from other bugs, the company was able to engineer synthetic metabolic pathways—ones that do not exist in nature—that enable the bacteria to produce isoprene from the sugars found in plant materials such as sugar cane, corn cobs and switchgrass, a tall-growing variety native to North America.

Genecor的科学家们将其它细菌的基因拼接到大肠杆菌上，从而在大肠杆菌上制造出一种自然界中不存在的代谢物合成途径。它使细菌能从植物材料中的糖分生产出异戊二烯——这些含有糖类的植物包括甘蔗、玉米芯，还有原产于北美洲的高个儿品种柳枝稷。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

Specifically, the reported difficulty in dissociating the human ES/EG cell clusters into viable single cells is problematic, particularly for gene–targeting experiments.

具体来说，报告难以分离的人类胚胎干/乙二醇细胞簇成可行的单细胞是有问题的，尤其是对基因打靶试验。

For five Enterobacteriaceaes tested for ESBLs by double-disk method, two primers were designed and synthetized: TEM-1and SHV-1, ESBLs gene type was amplified, sequenced and analyzed by PCR; the minimal inhibitory concentrations of Cetiofur combined BLI-Tazobactam to the five Enterobacteriaceae according to different proportions were carried out with two fold dilution method.

对产生ESBLs的5株致病性大肠埃希氏菌，设计并合成TEM-1和是SHV-12对引物，用PCR方法进行了ESBLs的DNA扩增、测序和基因型分析；用试管二倍稀释法测定头孢噻呋与BLI-他唑巴坦钠以不同配比对产生ESBLs大肠埃希氏菌的最小抑菌浓度。

Compared to the flat culture, cells in 3-D culture more closely mirror what happens in living organisms in morphology, cell alinements, polarization, microenviroments, gene expression pattern and other biological characters. Therefore,3-D culture is useful to investigate the molecular mechanism of nasopharyngeal carcinogenesis.

因与传统的二维培养相比，细胞在三维培养中的形态、排列方式、极性及微环境更接近于体内状态，其基因表达和生物学行为也更接近于体内状态，因而建立能反映鼻咽癌发病不同时期的体外三维培养模型对于鼻咽癌的发病机制研究具有重要价值。

Simultaneously, the activity and coding gene expression of ribulose 1, 5-bisphosphate carboxylase, phosphoenolpyruvate carboxylase, and glycollic oxidase all decrease in two rice accessions under low potassium supply while significant decrease in non-allelopathic rice "Lemont".

水稻光合作用相关酶的分析结果表明，低钾条件下核酮糖－1，5-二磷酸羧化酶、磷酸烯醇式丙酮酸羧化酶和乙醇酸氧化酶的活性及其基因表达强度均降低，但化感水稻&PI312777&的下降幅度显著小于非化感水稻&Lemont&。

Highly conserved sequence from G46 to D104 existed in the middle of the translated a mino acid sequence, including an iron-binding motif (H3-D-H-L-V-C-L-D-C-G) with rich Histidines, No 'Fur-box' homology to the E.

在编码序列的上下游，RBS序列、-10序列、-35序列和二个潜在的转录终止子分别得到确定，但没有发现存在于大肠杆菌fur基因中的&铁盒子&。

Twelve microsatellite markers selected from the GENE-BANK were analyzed for polymorphism in silkies. Allele frequencies were estimated based on band presence or absence. Average heterozygosity and polymorphism information content were calculated by allele frequencies, and then, linkage analysis between character and markers by least squares means and the correlation among the 6 characters of egg traits in silkies were studied.

本试验选用12对微卫星引物，通过对丝羽乌骨鸡多态性扩增，计算出这些微卫星标记座位的等位基因频率、多态信息含量、群体杂合度；通过标记多态性与产蛋性状的最小二乘分析和多重比较，进行了分子标记与产蛋性状的相关分析，同时计算了产蛋性状间的表型相关。

Construction of heavy chain transgenic mice and genome type analysis Linearized transgene plasmids were microinjected into pronuclei of zygotes from CBA×C57BL/6 mice, and transplamted into oviduct of pseudo-pregnant mice.

二、抗体重链转基因小鼠建立及基因型分析将线性化的质粒显微注射入CBA×C57BL/6小鼠的受精卵细胞的雄前核内，然后导入假孕雌鼠的输卵管。

For the E. coli lac operon, write the partial diploid genotype for a strain that will produceβ-galactosidase constitutively and permease by induction.

以大肠杆菌的乳糖操纵元为例，请写出一菌株的部分二倍体基因型：β-半乳糖苷酶是组成型表达的，透过酶是诱导表达的。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。