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The study on the phylogenetic relations among these species is very useful for the study on genome and marker-assisted breeding in Brassica species. The karyotypic analysis is one of the most important methods in analysing the phylogenetic relation of species with closer relations. The protocol for inducing high frequency metaphase was established by pretreatment with low temperature and 8hydroxyquinoline, and then the preparation of chromosome and karyotypes analysis of six economical important Brassica species were carried out. According to the karyotypic analysis, it was shown that in the genomes of amphidiploid species, there are genomical components from didiploid species. Our result showed that B. campetris, B. juncea and B. napus belonged to class 2B, while B.

以二倍体B.nigra(B,n=8)基因组为探针,用B.campestris(A,n=10)或B.oleracea(C,n=9)的基因组进行封阻,分别与B.juncea(AB,n=18)和B.carinata(BC,n=17)进行GISH杂交发现,B.juncea和B.carinata基因组中仅有来自于B.nigra的8对染色体表现杂交信号,而其它染色体杂交信号较弱;以B.campestris或B.oleracea基因组为杂交探针与B.napus(AC,n=19)进行GISH杂交发现,所有19对染色体均出现杂交信号,根据杂交结果难以正确判断B.napus基因组中的哪些染色体是来自于A基因组,哪些是来自于C基因组;以rRNA基因(18S-5.8S-26S)为探针的FISH杂交结果表明,B.campestris基因组中rRNA基因位点为6对;B.oleracea为3对;B.nigra为2对;B.carinata为4对;B.juncea为6对;B.napus为6对。

In addition,about 2/3 genes of human being which cause disease including genes relative to cancer,can be found in Drosophila as similar genes.

此外,在对果蝇和人类基因组的比较中发现,三分之二引发人类疾病的基因在果蝇中都存在类似的基因。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物,经过扩增前导序列10~34aa基因序列,并重新克隆入质粒pRSET-A构建串联二聚体后,再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点,经一系列简单的酶切和连接,快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因,并成功表达其六聚体重组蛋白。

The other classfies the calculation of MBPS into the solution of inequation based on heuristic knowledge of MBPS of optimal directional coordination setting in complex multiloop network and comes into equivalence of 0-1 integral second layout problem. On the basis of the principle of Gene Optimal Evolution, a new approach to determine MBPS is first presented and decreases the computational complexity of MBPS problem.

其二,利用复杂环网方向保护最优整定配合中最小断点集的启发知识,将MBPS的计算归结为解不等式,进而等价为0—1整数二次规划问题,基于基因遗传优化计算原理,首次提出了MBPS问题的基因遗传算法,降低了问题的计算复杂性。

According to Mather and Jinks model, using generations mean analysis m eth od, the various kinds of gene effects and its percentage of gene-effect-square s um were estimated,and comparative analysis on the gene effects and the importan ce t o inheritance of characters was carried out between the supernumerary spikelet l ines.

黄毅以主穗小穗数在25~30个的二个普通小麦多小穗品系85DH5015、SW94-30921 与三个普通小麦品种组配成6个组合的P1、P2、F1、F2、B1、B2为材料,用世代均值法分析了6个性状的遗传模型并估算了各类基因效应值,比较分析了两个不同来源多小穗小麦品系六个性状各类基因效应的异同及其相对重要性。

In them,as for the mean value calculated by least square method,AA maximum,AB next and BB minimum.In addition,GH3 obviously affected the body length,AA maximum, AB next and BB minimum.

运用最小二乘法对三个牦牛群体进行分析发现GH基因第3外显子对体重有显著影响(P<0.05),其中,最小二乘法得出的均值,AA型最大,AB型次之,最小的为BB型。

Gene ethics is an ethics of high technology in the form of a revolution in moral philosophy.

基因伦理是一种道德哲学革命形态的高技术伦理,具有技术革命与道德哲学革命的二重形态和二重本质。

In this study, 12 stylo genotypes were surveyed under field conditions with or without P application for P efficient screening, and then sand and nutrient solution culture experiments were subsequently conducted to further demonstrate possible physiological mechanisms of stylo in adaptation to low-P acid soils.

本试验选用十二种不同基因型柱花草作为研究对象,通过田间试验对供试材料进行磷效率初步筛选,并在此基础上进行随后的砂培和水培试验,对不同基因型柱花草适应酸性低磷红壤的机制进行了初步的探讨。

The body does not keep track of how it solves a problem, so it cannot pinpoint which genes to pump up the muscle on the blacksmith's biceps, or which genes regulate respiration and blood pressure.

身体并不记录自己是如何解决问题的,因此并不能准确指出哪种基因用来在铁匠的二头肌上给肌肉打气,或者哪种基因用来调节呼吸和血压。

Moreover, the distinguised advantages of applying the bivalents instead of somatic chromosomes to carry out the studies of fish gene mapping were discussed in details, and a few of new tentative ideas about how to develop the gene mapping in fish deeply were primarily postulated and theoretically analyzed in this paper.

此外,还详细讨论了以鱼类二价体替代体细胞染色体开展基因定位研究的突出优点与必要性;地高辛标记原位杂交技术和PRINS技术的特色与应用潜力;染色质风韵与等同区等新概念;以及如何深入开展鱼类基因定位研究的新设想。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

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