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We have researched distribution of TGF β and its receptor in various age group and discs of various degeneration grade, found that positive ratio of TGF β and its receptor is higher in below 40 year old group and discs of MRI Ⅰ and Ⅱ grade, mainly occur in fibrous cartilagous cells of fibrous annulus and nucleus pulpous; In group of 40-60 years old and grade Ⅱ and Ⅲ discs, TGF β decreased and TGF β R decreased not as much as TGF β; In above 60 years old group, TGF β and its receptor almost disappear.

生长因子包括TGF-β对细胞功能有重要调节作用,它是一种相对分子质量为2.5×10〓的二聚体多肽,几乎参与了哺乳动物所有细胞的病理,生理过程,尤其是在细胞外基质的产生及调控方面意义重大,且TGF-β已被公认为是与胶原代谢关系最密切的细胞因子。胡有谷、陈宏等,应用杂交技术检测了TGF-β对椎间盘Ⅰ、Ⅲ型胶原MRA的调节作用。

GluLI was very weak at birth with very light staining of Purkinje cells and a very few granule cells.

二周时不光蒲氏细胞和它的树突,部分分子层细胞和GolgiⅡ细胞也有存在,颗粒细胞的阳性数还不多。

It is determined that the contents and distribution of pyrrolic compounds and dibenzothiophene are controlled by oil fraction during migration, which has potential significance in evaluating the oil migration and reservoir Filling direction.

杂环芳烃中的吡咯类化合物和二苯并噻吩具有显著的油气运移分馏效应,可以作为有效的油气运移与油藏充注方向的分子级示踪参数。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子,用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库,ELISA鉴定阳性克隆,DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列;免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

Then, nanoparticles were prepared with a reprecipitation method and tested. We found that the nanoparticles with small size and lower poly-dispersion index can be prepared though shortening deposited time, decreasing concentration, increasing deposited temperature and adding surfactant.

利用二次沉淀法制备出三种有机分子不同粒径的纳米微粒,通过改变时间、浓度、温度、表面活性剂等实验条件分析这些因素对纳米微粒粒度的影响,发现缩短放置时间、减少有机稀溶液的注入量、升高放置温度、加入表面活性剂可以得到小粒度,低分散度的纳米微粒。

Rhodanine derivatives have been used for noble metal detection since long before, yet only one example was reported for anion sensing.

本论文中,我们设计合成了两个结构简单的基于绕丹宁的阴离子受体分子ARD和FRD,分别以蒽和二茂铁基团作为信号报告基团。

In the process of clearing buildings, Iraqi National Guardsmen reportedly arrested 25 insurgents inside a mosque in Samarra.

据报道,在清理建筑物的过程中,伊拉克国民自卫军在萨迈拉的一个清真寺里逮捕了二十五名反叛分子。

Laboratory of Gellular and Molecular Biology, Shaaghai Medical College, Fudan University, Shanghai 200032, ChinaAbstract:Objective Using sodium N-lauroyl sarcosine as new decellularization reagent to prepare decellularized matrix for tissue engineering purpose.

吴春根 王松梅高红阳潘銮凤复旦大学上海医学院细胞与分子生物学实验室,200032摘要:目的尝试用十二烷基肌氨酸钠作为脱细胞试剂,制备脱细胞组织工程支架材料,并分析其生物学性能。

The results showed that intermidiate 1, 2-propanediol-sebacate of low relative molecular mass was synthesized with toluene-p-sulfoinc acid as catalyst, benzene as watercarrying agent, and of mole ratio of sebacic acid to 1, 2-propanediol of 1:4. The glass-transition temperatures of PPSC were below 0 ℃ which confirmed that PPSC would be in a high-elastic state of ambient temperature and human body temperature. PPSC was a elastomer with moderate crosslinkage, low module and high elongation, and had rapid degradation ratio (degradation degree more than 50%) in phosphate (pH value 7.4) at 37℃.

结果表明,以对甲苯磺酸为催化剂、苯为带水剂,在酸醇比为1,4的条件下可合成较低相对分子质量的中间体癸二酸聚酯二元醇;PPSC弹性体的玻璃化转变温度在0℃以下,于室温及人体温度下处于高弹态;PPSC为适度交联的、低模量高伸长的弹性体,其在37℃、pH值为7.4的磷酸盆缓冲溶液中具有较快的降解性(降解度超过50%)。

The instrument may be used in measuring sheat wave resonant absorption spectrum of liquid film in 1x10 to the power -5 -500 Hz frequency range and in providing measurement data for the research of the interaction range between clusters, long chains and multibands of liquid film, the group behavior of molecules and clusters, the long chain in polymer solution, the second, the third and the fourth group behavior in left liquid and other group behavior in chemical solution and complex.

本发明的谱仪可在10 -5 ―500Hz频率范围内,测量液体膜的切变波共振吸收谱,为研究溶液中的团簇间、长链间和多键间的交互作用范围,分子和团簇的集团行为,高分子溶液中的长链,生命液体中的二、三、四级或其他化学溶液和络合物中的基团的行为提供可靠的测量。

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