二分子

By means of covalent coupling with the activation of 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide.

利用自组装技术将三巯基丙酸固定在金电极上，以1-乙基-3-（3-二甲氨基）碳二亚胺盐酸盐和N-羟基硫代琥珀亚胺作为活化剂，将甲基对硫磷抗体以共价键形式固定在三巯基丙酸自组装单分子膜修饰的金电极上，制备了一种免疫传感器。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

With increasing monomer concentration inside the disperse phase, the monodispersity of emulsions became slightly worse and the mean diameter of emulsions gradually became smaller. Monodisperse monomer-containing emulsions were obtained when the SPG membrane pore size was larger than 1.0 μm; however, when the SPG membrane pore size was smaller than 1.0 μm, no monodisperse emulsions were obtained because of the formation and chokage of p-phthalic acid crystals in the pores of the SPG membrane.

当SPG膜孔径大于1.0 μm左右时，可得到单分散的含TDC单体乳液；而当孔径小于1.0 μm左右时，水分子更容易扩散并溶解到油水界面甚至油相内部与TDC生成对苯二甲酸，TPA积累到一定程度在油水界面上析出将膜孔堵塞，从而无法制得单分散乳液。

It caused aggregation of purple membrane fragments at very low concentration. This effect might be attributed to its positive charges because cationic surfactant DTAB acted the same. On the other hand, melittin seemed to have behavior similar to CHAPS which has a rigid cholane ring,both had little effect on the conformation of retinal chromporphores.In consideration of the two-dimensional crystalline structure of purple membrane,melittin may form a rigid conformation such as α-helix on the surface of the membrane.

通过与三种在结构和电荷上不同的表面活性剂与紫膜的作用相比较，可以年出蜂毒作为带正电荷的分子与同样带正电的表面活性剂DTAB在引起紫膜凝聚方面表现相同；但在对紫膜可见光区的吸收光谱和圆二色谱的影响上却与具有刚性结构的CHAPS相似，表明蜂毒可在紫膜表面以一种刚性较大的构象存在，不能进入膜蛋白流水区的深层。

The molecule has a typical D-π-A structure with terminal units of electron donor and accepter groups. The triphenylamine group, as the electron donor, is connected to the oxadiazole group which is the electron accepter through the cinnamene group.

该分子具有典型的D-π-A型推拉电子结构，三苯胺基团作为给电子基通过苯乙烯共轭链桥接到吸电子基1，3，4-恶二唑基团。

The results obtained from Gel Permeation Chromatagraphy showed that, as time dapsed, gel's molecular weight changed increasingly. In other words, gel reaction of sebastan plum cordia was still continnous in 20 days after picking up. Based on AIS percentage analysis of sebastan plum cordia, gelation could be caused from much compounds like polysaccharase and protein.

由GPC分析结果显示，随著时间之增长，分子量的变化由小分子量逐渐凝结成大分子量，因此可知破布子在采收后二十天中其凝胶反应是持续在进行，且由破布子AIS百分比分析结果推测凝胶物质可能是多醣体或蛋白质等分子。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

In order to exploit the resources ofCrocus sativus and crocin further, molecular biological research of saffron wasstudied in the thesis.

为了研究西红花药用成分西红花甙的资源，更好地利用西红花甙，本论文对西红花进行了分子生物学方面的研究：对西红花酸合成酶(7，8-二加氧酶)基因的克隆，并将其在大肠杆菌中表达，纯化诱导蛋白，制备相应的特异性抗体。

Knoevenagel condensation reaction between aromatic aldehyde, malononitrile or ethyl cyanoacetate is promoted by organocatalyst N-methylimidazole.

以1-甲基咪唑为有机小分子催化剂催化芳香醛与丙二腈或氰乙酸乙酯的Knoevenagel缩合反应，反应速度快，操作方便，产率高，后处理简单。

Aim To investigate the production and removal of cyclobutane pyrimidine diners after UVB irradiation and the effect of baikal skullcap root, szechwan lovge rhizome and epigallocatechingallate on the irradiation damage in HaCaT cells, and finally, to clarify the probable regulatory mechanisms. Methods HaCaT cells were cultured in RMPI-1640 medium with 10% fetal bovine serum. The time and dosage of UVB irradiation were conducted according to the experimental design. Tested medicines were added into the medium before or after UVB irradiation.

目的 观察中波紫外线辐射后HaCaT细胞光产物胸腺嘧啶二聚体(cyclobutanepyrimidine dimers， CPDs)的生成和清除情况及3种中药活性成分：黄芩(baikal skullcap root， BSR)、川芎(szechwan lovge rhizome， SLR)和表没食子儿茶素没食子酸酯[-epigallocatechin-3-gallate， EGCG]对该过程的干预情况并探讨上述药物对相关调控分子的作用机制。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新