二倍的

Adopting duple diluent method and stiletto method and external antibacterial test on escherichia coli, solmonella paratyhpi , staphylicoccus aureus, streptococcus,, pseudomonas aeruginosa , Erysipelothrix rhusiopathiae, pasteurellosis to get the MIC and MBC, we studied antibacterial effect of the Copmounds Solatium Iyratum Thunb injection.

本研究采用试管二倍稀释法和打孔法对大肠杆菌、沙门氏菌、金黄色葡萄球菌、链球菌、绿脓杆菌、丹毒杆菌、巴氏杆菌的最小抑菌浓度和最小杀菌浓度及体外抑菌试验，研究复方白毛藤注射液抗菌作用。

Objective To observe effects of Tuber Fleeceflower Root serum on activity of SOD, the expression of c-myc mRNA and the activity of telomerase of human fetal lung diploid fibroblasts (2BS).

目的 观察含何首乌血清对人胚肺二倍体成纤维细胞（2BS）超氧化物歧化酶活性、c-myc mRNA表达及端粒活性的影响。

Objective To observe effects of Tuber Fleeceflower Root serum on activity of SOD, the expression of c-myc mRNA and the activity of telomerase of human fetal lung diploid fibroblasts (2BS).

作者：李朝敢，韦星，农嵩，韦耀东，张树球目的观察含何首乌血清对人胚肺二倍体成纤维细胞（2BS）超氧化物歧化酶活性、c-myc mRNA表达及端粒活性的影响

By using SRAP technology, a polymorphism primer of E2M5 was successfully selected. The primer E2M5 can be used to test hybrid purity of a small fruited diploid watermelon variety Hongxiaoyu.

采用SRAP技术，成功筛选出1对多态性好的引物对E2M5，对二倍体小果型西瓜品种红小玉杂交种纯度开展了分子鉴定研究。

The proliferation of SGC-7901 cell line was markedly inhibited by shRNA-GC-CⅢ（ P .05）. There was changes in cell morphology, such as cell volume reduction, karyopyknosis and chromatin conglomeration. FCM showed that GC-C inhibition caused the appearance of sub-G 0 /G 1 peak which increased from 5.47%（48h after transfection） to 5.63%（72h after transfection）.

转染GC-C shRNA真核表达载体后SGC-7901细胞的增殖能力受到抑制（ P 。05）；细胞形态发生改变，细胞体积缩小，核固缩，染色质浓缩聚集于核膜；流式细胞术检测显示G 1 峰前出现亚二倍体峰，转染48、72h后凋亡率分别为5.47%和5.63%（ P nbsp； 0.05）。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果：细胞接触肽段后存活率下降28.9％；光镜下可见细胞贴壁不良，胞体缩小，细胞突起断裂缩短；荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞；电镜下可见胞质中出现空泡样结构，细胞染色质浓集于核膜内侧并裂解成碎块状；流式细胞仪检测细胞出现亚二倍体峰，DNA电泳出现梯状带。

In this study, we show that Oryza australiensis ,a wild relative of the Asian cultivated rice O.

在野生稻里，澳洲野生稻属于EE基因组中的二倍体类型。

Such diplontic life cycles are found in some protoctists , such as the diatoms and certain Phaeophyta brown algae , including the wracks .

这样的二倍体生活史存在于一些原生生物中，例如硅藻以及一些褐藻。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

Furthermore, HBsAg gene expression was obtained in zygotic sporophytes by using male gametophytes-targeted bombardment.

继而利用雄配子体途径也实现了HBsAg在海带二倍体孢子体中的稳定表达，提示利用海带表达口服乙肝疫苗具有一定可行性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。