二倍核

Studies showed that the Giemsa-C banding patterns of Ae.tauschii- H.vulgare amphidiploid is much in accord with that of its two parents, and this further proved that the regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were spontaneously chromosal doubling amphidiploid.

分析表明，节节麦-大麦双二倍体Giemsa--C带核型与两个亲本的带型基本一致，这证明了节节麦-大麦双二倍体的染色体组成。

Blood samples from 23 Huacaya alpacas ,3 males and 20 females ,were used to study chromosome karyotype ,so as to provide some effective cytogenetics basis for the selectioiymprovement by crossing and disease diagnosis of alpacas ,and genetic mechanisms of sex determination. Peripheral blood lymphocyte culture was used to prepare chromosome. Methods of trypsase-EDTA and BSG (Barium hydroxide / Saline / Giemsa)were used for G-banding and C-banding respectively . The results showed as follows:Firstly , the number of diploid chromosomes was in the alpacas 2n=74,with the karyotype 74, XY and 74, XX for males and females respectively.

为了从选种、杂交改良、疾病诊断以及性别决定的遗传机制等方面为羊驼的繁育与推广提供更为有效的细胞遗传学资料，本试验采用外周血淋巴细胞培养法制备染色体，分析了23只胡阿基亚型羊驼(Huacaya alpaca，雌20只，雄3只)的染色体及其核型，并通过BSG法(Barium hydroxide／Saline／Giemsa，氢氧化钡-盐溶液-吉姆萨，也即Sumner BSG法)与胰酶-EDTA法分别对羊驼的C-分带和G-分带进行分析，结果表明：(1)羊驼二倍体染色体数目为2n=74，雄性羊驼核型为74，XY；雌性羊驼核型为74，XX。

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A：正常精子(精子头部有1个红色荧光信号者为X精子，有1个绿色荧光信号者为Y精子)；B：染色体数目异常精子两个绿色荧光信号同在1个精子核内，可为Y双体精子或二倍体精子，若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交，可以区别该精子属Y双体精子或二倍体精子

F〓 of transgenic allotetraploid fish containing"all-fish"transgene——pCAgcGHc was obtained respectively by inbreeding and gynogenesis. Positive rate of pCAgcGHc detected by PCR was 90％ in F〓 of transgenic allotetraploid fish produced by inbreeding, while most F〓 of transgenic allotetraploid fish produced by gynogenesis showed diploidy measured by flow cytometer. The results above imply that developing the pure line of transgenic allotetraploid fish through inbreeding is feasible, but the operation should be modified if gynogenesis is introduced.

采用近交和雌核发育方法培育出转pCAgcGHc基因异源四倍体鲫鲤F〓代，通过PCR检测，外源基因pCAgcGHc在近交转pCAgcGHc基因异源四倍体鲫鲤F〓中的整合率为90％，而流式细胞技术检测雌核发育转pCAgcGHc基因异源四倍体鲫鲤F〓的倍性多为二倍体，说明通过近交筛选转基因异源四倍体鲫鲤纯系的方法是可行的，但是通过雌核发育来筛选转基因异源四倍体鲫鲤纯系还要就方法上进一步的改进。

The results support the hypothesis of Polyploid gigantism and show the effect of polyploidy on nuclear size.

研究结果如下：一、三倍体牡蛎鳃上皮细胞核长轴、外套膜与唇瓣结缔组织中血淋巴核径的大小明显大于二倍体（P＜0.001），二倍体牡蛎直肠上皮细胞的高度与三倍体差异不明显（0.05＜P＜O.1）。

And the dipoid gynogenesis induction rates of the homologous sperm and the heterogenous sperm were 32.66%±7.03% and 28.00%±6.48%, respectively.

采用形态学、流式细胞仪DNA含量分析和微卫星标记技术对雌核发育鱼苗进行了分析，证明了雌核发育鱼苗为雌核发育二倍体。

At fertilization one male nucleus fuses with the gee nucleus to form the zygote, while the second male nucleus fuses with the primary endosperm nucleus to form a triploid cell, or more rarely a second diploid or a pentaploid cell that later gives rise to the ENDOSPERM.

受精作用发生时，一个精核与卵核融合形成受精卵，第二个精核与最初胚乳核融合，形成三倍体细胞，偶而也形成第二个二倍体或五倍体细胞，三倍体细胞最后发育为胚乳。

Results showed that the PMC meiosis of autotetraploid was similar to the diploid except some particularities. In metaphase Ⅰ, multivalent, quadrivalent, trivalent, bivalent and univalent were observed and some of the chromosomes were found not to rank on the metaphase plate and so did in metaphase Ⅱ cells. In anaphase Ⅰ and anaphase Ⅱ cells, there were lagging chromosomes, chromosome bridge and fragment. During anaphase Ⅱ and telophase Ⅱ, chromosome segregation was not synchronous or equal. At tetrad stage, dyad, triad, tetrad with micronucleus and polyad appeared.

结果表明，同源四倍体花粉母细胞减数分裂过程与二倍体相比，中期Ⅰ染色体构型复杂，有多价体、四价体、三价体、二价体和单价体；中期Ⅰ及中期Ⅱ有部分染色体没有排列在赤道板上；后期Ⅰ及后期Ⅱ出现落后染色体、染色体桥及断片；后期Ⅱ和末期Ⅱ有染色体不同步分离及不等分裂的现象；四分体时期还出现二分体、三分体、含微核的异常四分体及多分体。

Generally, two hybridization events have happened in the generation of gynogenetic crucian carp: the first one occurred about 6, 000 years ago when maternal ancestor of C. a. gibelio hybridized with males of C. a. auratus (ancestor of C. a. colored variety) and a diploid hybrid was produced; the second one happened over 10, 000 years ago, when the diploid hybrid was backcrossed with one of its parental population, by which the current triploid gynogenetic crucian carp emerged.

初步推断出东北雌核生殖银鲫是通过鲫鱼种群间的两次杂交产生的，第一次杂交是母本的银鲫同源种群与父本的彩鲫同源种群杂交，形成二倍体杂种；第二次杂交是二倍体杂种与两个祖先鲫鱼种群之一回交，形成了今天的三倍体杂种银鲫。

Pitardii,all from the Jinshajiang Valley of Yunnan and Sichuan, were cytologically studied in this work. Among 34 populations of C.reticulata studied here, 21 were found to be tetraploids (2n=60), 11 hexaploids (2n = 90), and the remaining two diploids (2n = 30). Tetraploid was reported in C. reticulata for the first time and its karyomophology was studied.

reticulata及其两个近缘种(怒江山茶C.saluenensis和西南山茶C.pitardii)进行了细胞学研究。34个居群的云南山茶花中，21个居群是四倍体类型(2n=60)，11个居群是六倍体类型(2n=90)，另2个居群为二倍体(2n=30)，云南山茶花的四倍体类型为首次发现，并且进行了核形态研究。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。