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二倍体

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Studies showed that the Giemsa-C banding patterns of Ae.tauschii- H.vulgare amphidiploid is much in accord with that of its two parents, and this further proved that the regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were spontaneously chromosal doubling amphidiploid.

分析表明,节节麦-大麦双二倍体Giemsa--C带核型与两个亲本的带型基本一致,这证明了节节麦-大麦双二倍体的染色体组成。

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A:正常精子(精子头部有1个红色荧光信号者为X精子,有1个绿色荧光信号者为Y精子);B:染色体数目异常精子两个绿色荧光信号同在1个精子核内,可为Y双体精子或二倍体精子,若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交,可以区别该精子属Y双体精子或二倍体精子

The increased number of mitochondria in tetraploids seemed to adapt the demanding of more energy for fecundation in tetraploids because of larger size. 3 Biochemical compositions in gonad and adductor muscle between diploids and triploids in the Pacific oyster, Crassostrea gigas, during an annual period were examined.

三、对二倍体与三倍体太平洋牡蛎的性腺和闭壳肌生化组分含量2003.4-2004.3的年度变化的分析结果显示:二倍体牡蛎性腺糖原含量在配子发生过程中明显降低,至性腺成熟时降低了78.57%(P<O.01),繁殖期维持在较低水平上,繁殖期过后逐渐升高,10月份至翌年3月份都保持较高的水平。

The results support the hypothesis of Polyploid gigantism and show the effect of polyploidy on nuclear size.

研究结果如下:一、三倍体牡蛎鳃上皮细胞核长轴、外套膜与唇瓣结缔组织中血淋巴核径的大小明显大于二倍体(P<0.001),二倍体牡蛎直肠上皮细胞的高度与三倍体差异不明显(0.05<P<O.1)。

The number of chloroplast in stomata guard cell, density, length and width of stomata were observed.

以苹果和梨的二倍体和多倍体为试材,比较了气孔保卫细胞叶绿体数目、气孔密度、气孔长和气孔宽四个性状与倍性的关系,分析各个性状用于倍性鉴定的可靠性,并采用两类性状同时进行判别分析,建立判别分析方程。

The effects of different light quality (blue light, red light and mixed blue and red light) on propagation of young stem and taking root in Hetao honeydew melon are shown. In regenerative whole plants, domestication was taken and suitable transplanting condition was studied.

对形成的植株进行了单倍体植株的鉴定,同时对获得的单倍体植株进行了染色体的加倍和鉴定,获得了纯合二倍体,通过对获得的纯合二倍体经过田间比较和鉴定,筛选出了部分优良的单株和株系。

Results of chromosomic analysis showed that the chromosome of Beijing Fatty Chicken, Langshan Chicken and Small-tailed Han Sheep were mainly diploid and the individuals of diploid occupied 87.1%, 86.7%, 93.4% in population observed respectively. The chromosomic structure mutation was 1.5%, 0.8%, 1.4% respectively that different from chromosomic morpHology results reported by other researchers.

染色体分析结果表明,北京油鸡、狼山鸡、小尾寒羊成纤维细胞均以二倍体为主,二倍体染色体数目分别占所观察数目的87.1%、86.7%和93.4%;染色体结构变异率分别为1.5%、0.8%和1.4%;染色体形态与前人报道的其他品种相比略有不同。

A highly resistant primitive cultivated species Solanum phureja was employed to generate a mapping population to perform the bulked segregant analysis for screening of AFLP markers linked with the resistance. Another population which had genetic similarity to the mapping population was used for testing of the markers achieved.

以马铃薯青枯病高抗性的原始栽培种Solanum phureja获得的二倍体群体为作图群体进行AFLP标记的初步筛选,另选一个与作图群体有较大亲缘关系和相近遗传背景的二倍体群体对所获标记进行验证。

However, analysis of DNA methylation level indicated that the total DNA methylation levels were not significantly different between diploid and autotetraploid. Autotriploid showed a trend of low DNA methylation level. It implied that the alterations of DNA methylation levels were not linear with the autopolyploidy level in watermelon.

有趣地是,二倍体与四倍体西瓜相比DNA甲基化水准却未发生大规模的调整,而三倍体西瓜显示出较为明显的低DNA甲基化水准趋势,表明西瓜同源多倍化过程中其基因组DNA甲基化水准变化与其倍性不成线性关系。

Generally, two hybridization events have happened in the generation of gynogenetic crucian carp: the first one occurred about 6, 000 years ago when maternal ancestor of C. a. gibelio hybridized with males of C. a. auratus (ancestor of C. a. colored variety) and a diploid hybrid was produced; the second one happened over 10, 000 years ago, when the diploid hybrid was backcrossed with one of its parental population, by which the current triploid gynogenetic crucian carp emerged.

初步推断出东北雌核生殖银鲫是通过鲫鱼种群间的两次杂交产生的,第一次杂交是母本的银鲫同源种群与父本的彩鲫同源种群杂交,形成二倍体杂种;第二次杂交是二倍体杂种与两个祖先鲫鱼种群之一回交,形成了今天的三倍体杂种银鲫。

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