二倍体

In this study,Amplified fragment length polymorphism analysis was carried out by using 23 pairs of EcoRI-NN/MseI-NNN selective primers in amplification of the DNAs from reciprocal F_1 hybrids of Cucumis hystrix Chakr.and C.

为了研究细胞质背景对基因组序列丢失的影响，利用23对Eco RI-NN/Mse I-NNN选择性引物对Cucumishystrix与栽培黄瓜C.sativus的正反交F_1、异源双二倍体及亲本DNA进行AFLP分析。

The proliferation of SGC-7901 cell line was markedly inhibited by shRNA-GC-CⅢ（ P .05）. There was changes in cell morphology, such as cell volume reduction, karyopyknosis and chromatin conglomeration. FCM showed that GC-C inhibition caused the appearance of sub-G 0 /G 1 peak which increased from 5.47%（48h after transfection） to 5.63%（72h after transfection）.

转染GC-C shRNA真核表达载体后SGC-7901细胞的增殖能力受到抑制（ P 。05）；细胞形态发生改变，细胞体积缩小，核固缩，染色质浓缩聚集于核膜；流式细胞术检测显示G 1 峰前出现亚二倍体峰，转染48、72h后凋亡率分别为5.47%和5.63%（ P nbsp； 0.05）。

The morphological observation showed that the two monosomic addition lines could form head normally, but the characters existed difference between the addition- lines and diploid parent.

对2个单体异附加系的植株形态学性状进行了初步观察，结果显示2个附加系均能正常结球，但球形的形态与二倍体结球甘蓝存在明显差异。

In this study, we show that Oryza australiensis ,a wild relative of the Asian cultivated rice O.

在野生稻里，澳洲野生稻属于EE基因组中的二倍体类型。

Such diplontic life cycles are found in some protoctists , such as the diatoms and certain Phaeophyta brown algae , including the wracks .

这样的二倍体生活史存在于一些原生生物中，例如硅藻以及一些褐藻。

The extra pronuclear of triploid zygotes after IVF is from sperm and the zygotes have no implantation value.

IVF后3PN卵子中多余的原核多为精子来源，没有移植价值；ICSI后3PN中多余的原核非精子来源，ICSI后3PN中二倍体率与IVF相比显著增高，在患者ICSI后无2PN卵子时可考虑行植入前遗传学诊断。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

The somatic hybrids usually showed specific bands and combined all the bands of both parents. In combinations, some new bands were detected, and occasionally specific bands of parents were lost, which indicated that chromosome recombination and exchange happened after fusion. Additionally, in some combinations, specific bands from leaf parent were detected, which revealed the plants were of putative hybrid origin, because leaf protoplast cannot redifferentiate by both single culture and co-culture.

体细胞杂种一般表现为具有双亲的特征带，且综合双亲的所有带，但在部分杂种中检测到了双亲都没有的新带，也发现有丢失亲本的特征带或共有带的现象，说明融合后染色体发生了重组和交换；有的引物只检测到叶肉亲本的特征带，根据柑橘叶肉细胞无论单独培养还是共培养均不能再生的事实，可推测其为体细胞杂种或二倍体叶肉亲本型胞质杂种。

In order to analyze the conservation of maize centromeric satellite DNA and centromeric retrotransposon in the subspecies and relatives of Zea mays, dual fluorescence in situ hybridization was used to detect the existence and distribution of the above two repetitive sequences in Zea mays ssp. mexicana, Z. diploperennis, Z. perennis, Tripsacum dactyloides, Coix lacryma-jobi, and Sorghum bicolor.

为分析玉米着丝粒卫星DNA和着丝粒反转录转座子在玉米种的亚种及近缘种中的保守性，采用双色荧光原位杂交检测了这两种重复序列在墨西哥玉米、二倍体多年生类玉米、多年生类玉米、摩擦禾、薏苡、高粱中的存在和分布。

The DNA relative content of large unreduced pollen grains induced by colchicine was determined quantitatively with laser scanning confocal microscopy using the normal-sized pollen grains as control. The results suggest that the large unreduced pollen grains are diploid.

以正常花粉作对照，用激光扫描共聚焦显微镜对经秋水仙碱处理诱导出的大花粉进行了DNA含量的定量测定，初步确认该试验诱导的大花粉是一种二倍体花粉。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。