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二倍体

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GISH technique was applied to TAF46 a partial amphiploid (2n=8x=56) derived from a Thinopyrum intermedium×Triticum aestivum hybrid and to 6 of the possible disomic addition line derived from it to determine the genome origin of the alien genome and the addition lines.

应用GISH对小偃麦部分双二倍体TAF46(2n=8x=56)及其衍生的6个二体异附加系的中间偃麦草染色体组种类进行了分析、鉴定。

The chromosome number of the mutated tomato (2n=4x=48) was twice that of the control.

变异材料的细胞染色体(2n=4x=48)为二倍体材料的二倍。

Molecular genotyping is validated as a method to confirm a diagnosis of CHM by demonstrating androgenetic diploidy and to resolve p57-positive cases into diandric triploid PHMs, biparental diploid nonmolar specimens, and the rare CHM with aberrant p57 expression.

分子基因型检测显示父系二倍体能确诊CHM,并能将p57阳性病例分类确诊为雄性异型三倍体的PHMs、双亲二倍体的非胎块性病变和罕见p57异常表达的CHM。

The enzyme Ali 1 can differentiate and determine 54 different types of species, which most of the species are heterozygote and only 8 species are homozygote of a diploid.

由酵素Ali 1可确认54种地方种,都是二倍体的同质体或异质体,其中仅8种(大陆种有 6地方种、台湾有 2地方种)是同质体。

Studies on photosynthetic characteristics of Chinese cabbage satellite monosome, diploid or trisome;2. Characteristics of Photosynthesis in Seed Plants of Satellite Chromosome Monosome, Diploid and Trisome of Chinese Cabbage

以大白菜随体单体、三体和二倍体为试材,在自然条件下,对生殖生长期的功能叶的叶绿素含量、希尔反应活性、净光合速率、光合速率日变化、Rubisco活性等生理生化指标进行了测定,以研究同源染色体的附加和减少对光合特性的影响,为开展染色体工程育种提供参考。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数按摩明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用,加药48h后增殖的抑制率可达43%以上;(2)ATP处理的U937细胞周期发生改变,G1期细胞数明显增多,ATP作用24h时G1期前出现亚二倍体峰——凋亡峰,凋亡细胞数为3.4%,作用48h时凋亡细胞数增多为22.7%;(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜,逐渐向核膜外移动,进入胞浆内再移向质膜内外面,紧贴质膜外面再逐步脱离细胞体,进入细胞基质中,成为游离的凋亡小体。

The haploid plant produces gametes mitotically and is thus termed the gametophytes while the diploid plant produces spores meiotically and is called the sporophyte.

单倍体植物有丝分裂产生配子体,二倍体减数分裂产生孢子体。

Furthermore, HBsAg gene expression was obtained in zygotic sporophytes by using male gametophytes-targeted bombardment.

继而利用雄配子体途径也实现了HBsAg在海带二倍体孢子体中的稳定表达,提示利用海带表达口服乙肝疫苗具有一定可行性。

There was no abnormal behavior observed in meiotic chromosomes in carrot. It has simultaneous cytokinesis. There are nine bivalents (9II) at metaphase I, indicating it is a typical diploid species.

通过观察胡萝卜花粉母细胞的减数分裂过程,发现其行为正常,是同时型胞质分裂,中期Ⅰ有9个二价体(9Ⅱ),为典型的二倍体物种。

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