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The bivalents were not homogeneously labeled with this technique and the extension signals were very apparent and relatively stable,and these can be applied to identify the individual bivalent.

其结果显示黄鳝二价体上DNA延伸效果十分明显,银颗粒基本上是非随机性的相间排列,而且主要特征是基本稳定的,据此可以进行染色体识别。

At metaphase Ⅰ, 7 bivalents were frequently detected to separate into three groups, with a form of 3+2+2, which indicated that at least four translocation events once occurred.

在中期Ⅰ,7个二价体出现分组分布现象,主要以3+2+2形式存在,表明其至少经历了4次染色体易位。

As the Federation of cell chromosome by a 2n of Article n of univalents into bivalents, although the DNA content remained unchanged, but the number appears to reduce by half.

由于联会,细胞中的染色体由2n条单价体成为n条二价体,虽然DNA含量未变,但数目看起来减少了一半。

Moreover, the distinguised advantages of applying the bivalents instead of somatic chromosomes to carry out the studies of fish gene mapping were discussed in details, and a few of new tentative ideas about how to develop the gene mapping in fish deeply were primarily postulated and theoretically analyzed in this paper.

此外,还详细讨论了以鱼类二价体替代体细胞染色体开展基因定位研究的突出优点与必要性;地高辛标记原位杂交技术和PRINS技术的特色与应用潜力;染色质风韵与等同区等新概念;以及如何深入开展鱼类基因定位研究的新设想。

The events occurring during these stages differ in meiosis and mitosis, notably in that bivalents (pairs of homologous chromosomes) are formed in meiosis, whereas homologous chromosomes remain separate in mitosis.

在减数分裂和有丝分裂中,前期发生的事件是不同的,尤其是二价染色体区别最为明显,在减数分裂中形成,而在有丝分裂中同源染色体是分离的。

The dark bands alternating with light bands,relatively stable and R-band-like structure was showed on the pachytene bivalents of Rics-field eelby using the random-primed in situ digoxigenin-labelled DNA synthesis technique,and the results were analysed and discussed in detail.

在黄鳝二价染色体上,运用地高辛配基标记的随机引物原位DNA合成技术诱导出了明暗相间、基本稳定且类似于R带的带状结构。本文对该结果进行了较详细的分析和讨论。

In order to develop a new path for gene mapping of fish, the digoxigenin and tritium labeled in situ hybridization techniques combined with multiple banding analysis on the pachytene bivalents of M. albus were studied using the wheat ribosomal RNA genes, pig growth hormone gene and MaSoxl (M. albus SRY boxl) DNA segment as the probes. Moreover, the techniques of random primed in situ DNA synthesis were also developed on the bivalents of M.

以两种异源基因和Ma Soxl为探针,采用地高辛或氟标记的原位杂交技术,结合点渍法和DNA印渍法分析,率先在黄鳝减数分裂二价体上开展基因定位研究,并与多重带带型分析相结合,以期为构建黄鳝染色体框架图奠定初步基础,为迅速改变鱼类基因定位研究的落后局面而开辟新的途径。

Using 〓ryanodine binding assay, the effects of 〓 and divalent cation's chelators on ryanodine receptors of skeletal and cardiac muscle cells were investigated.

采用〓ryanodine结合的实验技术,本工作研究了锌离子和一些二价金属离子螯合剂对骨骼肌和心肌ryanodine受体的作用及其机制。

In the medium without copper and iron, onlywith ferric citrate , the expression of FDR3 could complement the growth defect of ctrl mutant .CTR1 is a member of high梐ffinity iron transport system .

酵母突变体中铁高亲和系统的成员Ctrl缺失突变,培养基中不供给二价铁和铜,仅以柠檬酸三铁作为唯一的铁源,FDR3基因的表达能够恢复Ctrl突变体在该选择培养基中的表型生长。

In this study, the early stage development of Japanese medaka was used to assess the toxic effects of potassium permanganate and its byproduct Mn(superscript 2+) in the drinking water process.

饮用水生产中投加高锰酸钾可能引起高锰酸钾及其副产物二价锰的毒性效应。

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