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Methods: The protein and other impurity in the sample were precipitated by zinc acetate and potassium ferrocyanide after dipping of the sample. N,N-dichlorocyclohexylamine derived from sodium cyclamate by sodium hypochlorite in the filtrate was extracted by hexane. The HPLC separation was carried out with a phenomenex Gemini-C18 column using acetonitrile-water(7+3) or methanol-water (8+2) as mobile phase and detected by UV monitor at 314 nm. External standard method was used for quantification.

样品中甜蜜素浸提后,用乙酸锌和亚铁氰化钾试剂沉淀蛋白质等干扰杂质,过滤,用次氯酸钠将样品溶液中的甜蜜素转化为N,N-二氯环己胺经正己烷萃取后,在phenomenex Gemini-C18柱上,以乙腈-水(7+3)或甲醇-水(8+2)为流动相进行分离,HPLC-UV检测器在314 nm测定,外标法定量。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。