二乙锌

Methods: The protein and other impurity in the sample were precipitated by zinc acetate and potassium ferrocyanide after dipping of the sample. N,N-dichlorocyclohexylamine derived from sodium cyclamate by sodium hypochlorite in the filtrate was extracted by hexane. The HPLC separation was carried out with a phenomenex Gemini-C18 column using acetonitrile-water(7+3) or methanol-water (8+2) as mobile phase and detected by UV monitor at 314 nm. External standard method was used for quantification.

样品中甜蜜素浸提后，用乙酸锌和亚铁氰化钾试剂沉淀蛋白质等干扰杂质，过滤，用次氯酸钠将样品溶液中的甜蜜素转化为N，N-二氯环己胺经正己烷萃取后，在phenomenex Gemini-C18柱上，以乙腈－水(7+3)或甲醇－水(8+2)为流动相进行分离，HPLC-UV检测器在314 nm测定，外标法定量。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。