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Methods Injecting quinolinic acid into rat hippocampus after injecting D-galactose to form the dementia rat model.

在s.c.D-半乳糖造成大鼠亚急性衰老的基础上,双侧海马内注射喹啉酸造成类AD模型。

This indicated that pgi gene is involved in gluconueogenesis. The virulence of pgimutant was tested on Chinses radish Raphanus sativus L. var. radical us Pers.

碳源利用检测结果表明,pgi突变体对多种碳源,例如柠檬酸、琥珀酸、延胡索酸乙酸、乳糖等的利用能力很差,这说明该基因在糖异生途径中起重要作用。

These uncontaminated explants showed that morphogenetic potential were 70%-75% and each responding explant had 1.2-1.4 shoots formation on MS medium supplemented with 2 mg/L NAA and 16 mg/L BA. Moreover, adding antibiotics (100 mg/L Rifampicin and 100 mg/L Gentamycin) to solidify MS contained 3% galactose were tested, results showed the lowest contamination rates were 20% and 1.0-1.2 shoots were regenerated per responding explant. The antibiotic treatments with 100 mg/L Rifampicin and 100 mg/L Gentamycin could reduce internal contaminants, while the addition of antibiotics into medium in vitro culture led to phototoxic phenomena of axillary explants, morphogenetic potential lost and the growth of regenerant was also inhibited.

以100 mg/L Rifampicin+100 mg/L Gentamycin或100 mg/L Rifampicin+100 mg/L Gentamycin+150 mg/L Streptomycin组合浓度处理者,具有显著降低污染率的效果分别为60%及50%;克服内生菌污染而建立初代无菌培养之腋芽培植体,则以100 mg/L Rifampicin或以100 mg/L Rifampicin+100 mg/L Gentamycin处理者,芽体再生率最高70%-75%,惟平均每一腋芽培植体芽体增殖数仅1.2-1.4个;另在醣种类及浓度与抗生素试验,显示100 mg/L Rifampicin+100 mg/L Gentamycin之抗生素浓度组合添加至含3g/L半乳糖的培养基,显著降低污染率至20%;但未污染之培植体,形态发生潜能低至40%以内,可反应生长之培植体芽体形成数也仅达1-1.2个之间,再生芽体之生长也显现抑制现象。

Objective To observe the replicative senescence-related beta-galactosidase activity in cultured human retinal pigment epithelium cells.

目的观察培养人视网膜色素上皮细胞中复制衰老相关的β半乳糖苷酶活性。

We further expressed the β-galactosidase reporter gene in R. ruber driven by promoter PlacZ. Results indicated that lacZ was an appropriate reporter gene for genetic or metabolic engineering research of Rhodococcus.

采用PlacZ启动子在红球菌宿主中启动b-半乳糖苷酶报告基因,结果表明, lacZ能够在红球菌中高效表达,是优选的红球菌报告基因。

Compared with the Pre-trial period, during the Trial period milk production, milk protein and lactose production significantly increased, ruminal pH decreased 6.64 vs.

与对照期比较,BSN2处理期奶牛产奶量显著增加,奶中乳蛋白和乳糖产量增加(P 0.05),瘤胃pH降低6.64 vs。

Objective: To investigate the effect of Epibueropyridinium A, extracted from Scrophularia ningpoensis, in prevention of D-galactose-induced cataract in rats.

目的:探讨玄参中环烯醚萜Epibueropyridinium A对D-半乳糖性白内障形成的干预作用。

According to Gas Chromatography infra-red spectra analysis, the four fractions all contain different quantities of arabinose , galactose, glucose and seminose, VLP-2 and VLP-3 also contain rhamnose and xylose, polysaccharide configuration may have B -D-pyran glucose.

以粗多糖为起始原料计算,四个级分的纯品多糖得率分别为:VLP—1为25.32%,VLP—2为50.82%,VLP—3为7.55%,VLP—4为10.41%。根据气相色谱分析表明,四个级分的多糖都含有不同量的阿拉伯糖、半乳糖、葡萄糖、甘露糖,VLP—2、VLP—3还含有鼠李糖和木糖。

To the same sample production, using the iodimtry method, freezing point decent method, HPLC and enzymic method, the difined hydrolytic percentage is 63.2%,62%,61%,61.8% separatedly.

对同一样品运用碘量法、冰点下降法、高效液相色谱法、酶催法进行乳糖水解率的测定,测得水解率分别为63.2%、62%、61%、61.8%。

Objective:To study the anti-senile effect of Sorbaria sorbifolia extract on Dgalgctose induced subacute senile in rats.

目的:探讨珍珠梅提取物对D半乳糖致衰老大鼠的保护作用。

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