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To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主,要求研究生学习和掌握一些现代微生物学研究所需的基本技术,内容包括:相差显微镜、荧光显微镜的使用和显微摄影技术,PCR技术的应用,细菌质粒的构建、转化及检测技术,病毒的侵染性及其分子检测技术,丝状真菌原生质体融合技术,大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术,细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

The contents of α-galactooligosaccharides and sucrose in different cultivars of chickpea seeds were determined with the aim of selecting those with high amounts of α-GOS and low contents of sucrose for use as ingredients in functional foods.

分析不同品种鹰嘴豆中α-低聚半乳糖与蔗糖的含量差异,以期获得α-GOS含量较高而蔗糖含量相对低的鹰嘴豆品种,为中国鹰嘴豆资源的有效利用提供理论依据。

Objective To study the effects of Compound 909 on the function of learning and memory as well as hemorrheology in D-galactose induced dementia rat model.

目的 观察复方 90 9对D 半乳糖痴呆模型大鼠学习记忆及血液流变学的影响。

Objective To study the effects of Compound 909 on the function of learning arid memory as well as hemorrheology in D-galactose induced dementia rat model.

目的 观察复方909对D-半乳糖痴呆模型大鼠学习记忆及血液流变学的影响。

In this dissertation, a series of cluster galactosides and cluster mannoside s were synthesized, which target asialoglycoprotein receptor and plant lectin Concanavalin A respectively.

本论文以肝细胞表面受体和植物凝集素Con A为靶点,研究了多价半乳糖簇和甘露糖簇的合成及生物活性。

The optimum pH is 8-10 for hypha growth and 7-9 for conidiospore germination; The hypha could not grow on the culture medium which nitrogen source comes from urea and asparagines, but it could grow fast and produce more conidiospore on the culture medium which carbon source comes from maltose, glucose and lactose, and nitro0gen source come from glycine, histidiane and peptone.

该菌在PH 4~12范围内均能生长、产生分生孢子并萌发,菌丝生长的最适pH 8~10。,孢子萌发的最适PH 7~9;该菌不能在以尿素和天门冬酰氨为氮源的培养基上生长,但以麦芽糖、葡萄糖、乳糖、淀粉、甘氨酸、组氨酸和蛋白陈为碳源和氮源时,菌丝生长快、产孢量大;光照处理对该菌菌丝生长、产孢和孢子萌发无显著影响。

Objective To study the influences of dichloromethane extraction from crude and processed Cornus Officinalis on bone marrow cells of aging mice induced by D-galactose.

目的 研究山茱萸生、制品二氯甲烷萃取部位对D-半乳糖致衰小鼠骨髓细胞的影响。

Objective: To study the effects of glucose, mannose and galactose on CAP10 promoter activity of Cryptococcus neoformans capsule-associated gene.

目的:研究葡萄糖、甘露糖、半乳糖对新生隐球菌荚膜相关基因CAP10启动活性的影响。

This scrutiny of the words peculiar to each play throws light on another difficulty in the Pastorals, viz, the recurrence of such expressions as "a faithful saying","sound words", etc."Moon-calf" occurs five times in "The Tempest", and nowhere else;"pulpit" six times in one scene of "Julius Csar" and never elsewhere;"hovel" five times in "King Lear";"mountaineer" four times in "Cymbeline", etc. Compare,"God forbid", me genoito of Gal., Rom., once in I Cor.

这一审议的话特有的每个发挥全光照对另一个困难是,在pastorals ,即再次出现这种表现为"忠实说","复音词"等"月亮-小牛"发生了5次"暴风雨",并没有其他";讲坛"的6倍在另一个场景中的"朱利csar "从来没有在其他地方"; hovel "的5倍,在"李尔王";"登山"的4倍,在" cymbeline "等比较,"但愿上帝阻止",我genoito半乳糖,光碟,一旦在I肺心病。

Then the changes of cell shape and ultrastructure were observed by microscope and transmission electron microscopy, and the percent of cells in the G1 phase and SA- ft -galactosidase activity were measured with flow cytometry and cytochemistry staining, respectively.

从光镜、透射电镜观察细胞形态及超微结构的变化;流式细胞术分析细胞周期,计算G1期细胞的比例;SA-β-半乳糖苷酶的细胞化学染色,确定成功建立体外细胞衰老的模型。

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