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Be diagnose diarrhoea disease and 125 little patients of blemish of carbohydrase of afterwards hair breast, random cent is mixed for remedial group contrast group.

将确诊为腹泻病并继发乳糖酶缺陷的125例患儿,随机分为治疗组和对照组。

Objective To investigate the effects of clausenamide derivative on carbon tetrachloride (CCl4) and Dgalactosamine induced acute liver injury in mice.

目的 观察合成的4苯基卤代黄皮酰胺衍生物对四氯化碳(CCl4)和D半乳糖致小鼠急性肝损伤的影响。

The method for direct preparation of sodium caseinate from Qula was iwestigated, soaking and washing conditions were studied and compared, Analysis on factors influencing test such as material concentration, washing times, soak temperature and soak time was carried out.

采用曲拉直接浸洗法制取酪朊酸钠,进行浸洗工艺条件研究比较,对原料浓度、水洗次数、浸泡温度、浸泡时间等影响因素进行试验分析,确定最佳工艺条件为:原料浓度11%,浸泡温度45℃,浸泡时间3.5h,水洗3次,最终产品乳糖含量为0.35%。

What consequences would a mutation in the catabolite activator protein gene of E. coli have for the expression of a wild-type lac operon?

大肠杆菌的代谢激活蛋白基因突变会对野生型的乳糖操纵元的表达产生怎样的影响结果?

The catabolite activator protein binds to the ___ to regulate expression of the lac operon.

代谢激活蛋白与___结合可调节乳糖操纵元的表达

We previously identified the lactose-inducible β-galactosidase gene in Bacillus megaterium ATCC14581 and found that it is subject to catabolite repression by glucose.

我们先前的研究证明巨型杆菌ATCC14581的β-galactosidase基因受到乳糖诱导并且会受到葡萄糖的抑制。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察,采用相差显微镜观察其生长情况,透射电镜观察细胞结构,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞,以MTT比色法描绘生长曲线、检测生长状态,组化法检测老化相关β-半乳糖苷酶,流式细胞仪分析细胞周期和增殖指数。3。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The most important representatives of disaccharides are sucrose, lactose, maltose and cellobiose.

二糖最重要的代表是蔗糖,乳糖,麦芽糖和纤维二糖。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。