乙酸钠

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

Water,dimethicone,propylene glycol,glycerin,cyclomethicone,ppg-15 stearyl ether,sorbitan oleate,stearic acid,cetyl alcohol,glyceryl stearate,ethylhexyl methoxycinnamate,peg-100 stearate,panthenol,phenoxyethanol,phospholipids,triethanolamine,methylparaben,oenothera biennis oil,imidazolidinyl urea,carbomer,c12-13 alkyl lactate,glycyrrhetinic acid,citrus aurantium dulcisoil,propylparaben,tocopheryl acetate,benzophenone-3,triclosan,disodium edta,ethylparaben,pentadecalactone,iris germanica root extract,retinyl palmitate,bisabolol,butylparaben,sodium hyaluronate,aesculus hippocastanumseed extract,ruscus aculeatus root extract,chlorhexidine digluconate,biosaccharide gum-1,citrus aurantium amaraoil,tocopherol,lecithin,ascorbyl palmitate,glyceryl oleate,BHT,citric acid,limonene,linalool

水， 地美司康，丙二醇，甘油，环甲硅脂， PPG-15 硬脂基醚，油酸己六酯，硬脂酸，十六醇，甘油硬脂酸， 4-甲氧基肉桂酸-2-乙基己基酯，聚乙二醇硬脂酸酯，维生素B5，苯氧乙醇，磷脂质，三乙醇胺，羟苯甲酯，月见草油，尿素醛，卡波姆， c12-13烷醇乳酸酯，甘草亭酸，甜橙油，羟苯丙酯，醋酸盐维他命E，二苯酮-3，玉洁新，乙酸乙二胺二钠，羟苯乙酯，环十五内酯，鸢尾花根萃取，维他命A酯，没药醇，羟苯丁酯，透明质酸钠，欧洲七叶树籽萃取，假叶树根萃取，洗必泰葡萄糖酸盐，多醣物质，苦橙油，维生素E，卵燐脂，抗坏血酸棕榈酸酯，甘油油酸酯，二丁基羟基甲苯，柠檬酸，柠檬精油，芳樟醇水：几乎所有护肤品成分第一位都是水。地美司康：即silicon，矽灵，一般添加于霜剂中，使用后皮肤手感光滑。该成分不可吸收。

Water,propylene glycol,butylene glycol,glyceryl polymethacrylate,peg-7 glyceryl cocoate,biosaccharide gum-1,phenoxyethanol,hydroxyethylcellulose,ppg-26-buteth-26,chondrus crispus,peg-40 hydrogenated castor oil,sodium hydroxide,methylparaben,benzophenone-4,disodium edta,citrus aurantium dulcisoil,sorbic acid,butylparaben,ruscus aculeatus root extract,glycyrrhiza glabraroot extract,aesculus hippocastanumseed extract,pentadecalactone,iris germanica root extract,rentinyl palmitate,tocopheryl acetate,sodium hyaluronate,citrus aurantium amaraoil,sorbitol,propylparaben,ethylparaben,limonene,linalool,yellow 5,red 4,red 33

水，丙二醇，丁二醇，甘油聚甲基丙烯酸酯，PEG-7 椰子油酸单甘酯，多醣物质，苯氧乙醇，羟乙基纤维素，PPG-26-丁醇聚醚-26，卡拉胶，PEG-40氢化蓖麻油，氢氧化钠，羟苯甲酯，二苯酮-4，乙酸乙二胺二钠，甜橙油，山梨酸，羟苯丁酯，假叶树根萃取，甘草根萃取，欧洲七叶树籽萃取，环十五内酯，鸢尾花根萃取，维他命A酯，醋酸盐维他命E ，透明质酸钠，苦橙油，山梨醇，羟苯丙酯，羟苯乙酯，柠檬精油，芳樟醇，黄5，红4，红33水：几乎所有护肤品成分第一位都是水。丁二醇：保湿剂及溶剂，质地温和

Water,cetearyl alcohol,butylene glycol,ceteth-20,glycolic acid,cyclomethicone,citric acid,sodium hydroxide,xanthan gum,lactic acid,phenoxyethanol,sodium citrate,magnesium aluminium silicate,citrus aurantium dulcisoil,disodium edta,pentadecalactone,methylparaben,iris germanica root extract,butylparaben,ethylparaben,propylparaben,silica,chlorhexidine digluconate.citrus aurantium amaraoil,limonene,linalool

水， 棕榈醇，丁二醇，鲸蜡醇聚醚 20，甘醇酸，环甲硅脂，柠檬酸，氢氧化钠，山羊胶，乳酸，苯氧乙醇，柠檬酸钠，硅酸镁铝，甜橙油，乙酸乙二胺二钠，环十五内酯，羟苯甲酯，鸢尾花根萃取，羟苯丁酯，羟苯乙酯，羟苯丙酯，二氧化硅，洗必泰葡萄糖酸盐，苦橙油，柠檬精油，芳樟醇

Water,c12-20 acid peg-8 ester,octyldodecanol,cetearyl isononanoate,butylene glycol,biosaccaride gum-1,peg-40 hydrogenated caster oil,ethylhexyl menthoxycinnamate,phenoxyethanol,potassium cetyl phosphate,macadamia ternifolia seed oil,dimethicone,carbomer,propylene glycol,glyceryl stearate,cocoglycerides,butyrospermum parkii,c12-13 alkyl lactate,methylparaben,benzophenone-3,pentadecalactone,sorbic acid,citrus aurantium dulcis oil,sodium hydroxyde,tocopheryl acetate,disodium edta,iris germanica root extract,retinyl palmitate,equisetum arvense extract,butylparaben,peg-8,propylparaben,ethylparaben,sodium hyaluronate,chlorhexidine digluconate,citrus aurantium amaraoil,tocopherol,ascorbyl palmitate,citric acid,ascorbic acid,limonene,linalool

水，c12-20 acid peg-8 ester，辛基十二烷醇，鲸蜡硬脂醇异壬酸酯，丁烯二醇，多醣物质，PEG-40氢化蓖麻油，4-甲氧基肉桂酸-2-乙基己基酯，苯氧乙醇，十六烷基磷酸钾，澳洲胡桃子油，地美司康，卡波姆，丙烯乙二醇，甘油硬脂酸，椰油脂酸甘油酯类，牛油果，c12-13烷醇乳酸酯，对羟基苯甲酸甲酯，二苯甲酮-3 ，环十五内酯，山梨酸，甜橙油，氢氧化钠，维生素E醋酸酯，乙二胺四乙酸二钠，鸢尾花萃取，维他命A酯，问荆萃取，对羟基苯甲酸丁酯，聚乙二醇- 8，羟苯丙酯，羟苯乙酯，透明质酸钠，洗必泰葡萄糖酸盐，苦橙油，维生素E ，维生素C棕榈酸酯，柠檬酸，抗坏血酸，柠檬精油，沉香醇水：几乎所有护肤品成分第一位都是水。辛基十二烷醇：界面活性剂、乳化剂、稠化剂，有一定刺激性

Water,propylene glycol,butylene glycol,glyceryl polymethacrylate,ascorbic acid,peg-7 glyceryl cocoate,biosaccharide gum-1,peg-40 hydrogenated castor oil,ppg-26-buteth-26,phenoxyethanol,hydroxyethylcellulose,xanthan gum,methylparaben,pentadecalactone,citrus aurantium dulcisoil,disodium edta,sorbic acid,tocopheryl acetate,butylparaben,retinyl palmitate,iris germanica root extract,sodium hyaluronate,ethylparaben,propylparaben,citrus aurantium amara oil,limonene,linalool

水，丙二醇，丁二醇，甘油聚甲基丙烯酸酯，维生素C衍生物，PEG-7 椰子油酸单甘酯，多醣物质，PEG-40氢化蓖麻油，PPG-26-丁醇聚醚-26，苯氧乙醇，羟乙基纤维素，山羊胶，羟苯甲酯，环十五内酯，甜橙油，乙酸乙二胺二钠，山梨酸，醋酸盐维他命E，羟苯丁酯，维他命A酯，鸢尾花根萃取，透明质酸钠，羟苯乙酯，羟苯丙酯，苦橙油，柠檬精油，芳樟醇

Purified water, euphorbia cerifera wax, glyceryl stearate, ammonium acrylates copolymer, butylene glycol, copernica cerifera wax, stearic acid, sucrose acetate isobutyrate, acrylates copolymer, bentonite, tromethamine, hexylene glycol, silk amino acids, cholesterol, glycine, synthetic wax, ethylhexyl glycerin, alanine, PEG-9 dimethicone, nylon-6, lecithin, silica, simethicone, caprylyl glycol, xanthan gum, propylene glycol, isostearic acid, sodium laureth-12 slfate, ceteareth-20, polyvinyl alcohol, sodium chloride, sodium acetate, disodium EDTA, benzyl alcohol, chlorphenesin, phenoxyethanol, methylparaben, ethylparaben, propylparaben, butylparaben, isobutylparaben, may contain /- mica, iron oxides (CI 77491, CI 77492, CI 77499), titanium dioxide (CI 77891), ferric ferrocyanide (CI 77150), ultramarines (CI 77007), blue 1 lake (CI 42090), bronze powder (CI 77400), aluminum powder (CI 77000), bismuth oxychloride (CI 77163), carmine (CI 75740), chromium hydroxide green (CI 77289), chromium oxide greens (CI 77288), yellow 5 lake CI 19140

水，小烛树蜡，硬脂酸甘油酯，丙烯酸铵共聚物，1，3-丁二醇，巴西棕榈蜡，硬脂酸，异丁酸醋酸蔗糖酯，丙烯酸酯共聚物，蒙脱土，二吡喃乙酸乙酯胺，已烯醇，丝氨酸，胆固醇，甘油，合成蜡，异丁基甘油，丙胺酸，PEG-9二甲基硅油，尼龙-6，卵磷酯，硅粉，硅氧烷，癸酰基乙二醇，汉生胶，丙二醇，异硬脂酸，月桂基醚-12硫酸钠，十六十八醇醚-20，聚乙烯醇，氯化钠，醋酸钠，EDTA二钠，苯氧乙醇，氯代苯酚，卞醇，尼泊金甲酯，尼泊金乙酯，尼泊金丙酯，尼泊金丁酯，尼泊金异丁酯，[可能还含有云母，铁黑(CI 77491， CI 77492， CI 77499)，钛白粉(CI 77891)，氰化亚铁(CI 77150)，靛蓝(CI 77007)，蓝1号(CI 42090)，青铜粉(CI 77400)，铝粉(CI 77000)，氯氧化铋(CI 77163)，洋红色素(CI 75740)，氢氧化铬绿(CI 77289)，氧化铬绿(CI 77288)，黄5号(CI 19140)]文章来自网络，不代表本网站立场，版权归原作者所有，转载请注明出处！

Professional sales companies operating bromide products: sodium bromide, sodium bromide, potassium bromide, potassium bromate, Tetrabromobisphenol A, Deca-BDE, ten PBDEs, DBDPE, hexabromodiphenyl Central dodecane, dibromo neopentyl glycol, neopentyl bromide three alcohols, three bromine phenol, octabromodiphenyl ether, brominated polystyrene, bromide, calcium bromide, dibromo succinic acid, dibromo-D ene glycol, bromide surfactants series: Dodecyl trimethyl ammonium bromide, tetradecyl trimethyl ammonium bromide, cetyltrimethyl ammonium bromide, octadecyl trimethyl ammonium bromide dodecyl trimethyl ammonium chloride, tetradecyl trimethyl ammonium chloride, cetyl trimethyl ammonium chloride, octadecyl trimethyl ammonium chloride, tetrabutyl ammonium bromide wholesale sales: dimethylolpropionic acid, DL-malic acid, isophthalic acid PIA, glycolic acid, adipic acid.

公司专业经营销售溴化物系列产品：溴化钠、溴酸钠、溴化钾、溴酸钾、四溴双酚A、十溴二苯醚、十溴联苯醚、十溴二苯乙烷、六溴环十二烷、二溴新戊二醇、三溴新戊醇、三溴苯酚、八溴醚、溴化聚苯乙烯、溴化铵、溴化钙、二溴丁二酸、二溴丁烯二醇、溴化表面活性剂系列：十二烷基三甲基溴化铵、十四烷基三甲基溴化铵、十六烷基三甲基溴化铵、十八烷基三甲基溴化铵、十二烷基三甲基氯化铵、十四烷基三甲基氯化铵、十六烷基三甲基氯化铵、十八烷基三甲基氯化铵、四丁基溴化铵批发销售：二羟甲基丙酸、DL-苹果酸、间苯二甲酸 PIA、羟基乙酸、己二酸。

Water; Glycerin; Sodium Laureth Sulfate; Polyethylene; Acrylates/C10-30 Alkyl Acrylate Crosspolymer; Lauryl Glucoside; Phenoxyethanol; Microcrystalline Wax; Fragrance; Methylparaben; Menthyl Lactate; Sodium Hydroxide; Tetrasodium EDTA; Cellulose; Lactose; Sodium Ascorbyl Phosphate; Acrylates/Ammonium Methacrylate Copolymer; Hydroxypropyl Methylcellulose; Mica; Talc; Titanium Dioxide; Tocopheryl Acetate; Triethyl Citrate; Cucumis Sativus Fruit Extract; Citrus Grandis Fruit Extract; Panax Ginseng Root Extract; Zingiber Officinale Root Extract; Iron Oxides; Red 30; Yellow 5; Yellow 5 Lake

水；甘油；钠Laureth硫酸盐；聚乙烯； Acrylates/C10-30烷基丙烯酸酯Crosspolymer ；十二烷基糖苷； Phenoxyethanol ；微晶蜡；香水； Methylparaben ；薄荷乳酸；氢氧化钠； Tetrasodium乙二胺四乙酸；纤维素；乳糖；钠抗坏血酸磷酸酯；丙烯酸酯/铵甲基丙烯酸共聚物；羟丙基甲基纤维素；云母；滑石粉；二氧化钛； Tocopheryl醋酸；三柠檬酸；黄瓜水果提取物；柚皮果提取物；人参根提取物；生姜根提取物；铁氧化物；红30 ；黄河5 ；黄河5湖

Water; Glycerin; Sodium Laureth Sulfate; Polyethylene; Acrylates/C10-30 Alkyl Acrylate Crosspolymer; Lauryl Glucoside; Phenoxyethanol; Microcrystalline Wax; Fragrance; Methylparaben; Menthyl Lactate; Sodium Hydroxide; Tetrasodium EDTA; Cellulose; Lactose; Sodium Ascorbyl Phosphate; Acrylates/Ammonium Methacrylate Copolymer; Hydroxypropyl Methylcellulose; Mica; Talc; Titanium Dioxide; Tocopheryl Acetate; Triethyl Citrate; Cucumis Sativus Fruit Extract; Citrus Grandis Fruit Extract; Panax Ginseng Root Extract; Zingiber Officinale Root Extract; Iron Oxides; Red 30; Yellow 5; Yellow 5 Lake.

成分Ingredients 水；甘油；钠Laureth硫酸盐；聚乙烯； Acrylates/C10-30烷基丙烯酸酯Crosspolymer ；十二烷基糖苷； Phenoxyethanol ；微晶蜡；香水； Methylparaben ；薄荷乳酸；氢氧化钠； Tetrasodium乙二胺四乙酸；纤维素；乳糖；钠抗坏血酸磷酸酯；丙烯酸酯/铵甲基丙烯酸共聚物；羟丙基甲基纤维素；云母；滑石粉；二氧化钛； Tocopheryl醋酸；三柠檬酸；黄瓜水果提取物；柚皮果提取物；人参根提取物；生姜根提取物；铁氧化物；红30 ；黄河5 ；黄河5湖

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。