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Pharmaceutical characteristics of dimethylamino ethyl(4-chlorophenylmethfoxy )acetate hydrochloridein rabbit was studied and several parameters at different timeand way of medicine administration were determined.

研究了4-氯代苯甲氧基乙酸二甲氨基乙酯盐酸盐在家兔体内的药代动力学,测定了不同时间不同给药方式家兔体内的血药浓度及动力学参数。

Professional sales companies operating bromide products: sodium bromide, sodium bromide, potassium bromide, potassium bromate, Tetrabromobisphenol A, Deca-BDE, ten PBDEs, DBDPE, hexabromodiphenyl Central dodecane, dibromo neopentyl glycol, neopentyl bromide three alcohols, three bromine phenol, octabromodiphenyl ether, brominated polystyrene, bromide, calcium bromide, dibromo succinic acid, dibromo-D ene glycol, bromide surfactants series: Dodecyl trimethyl ammonium bromide, tetradecyl trimethyl ammonium bromide, cetyltrimethyl ammonium bromide, octadecyl trimethyl ammonium bromide dodecyl trimethyl ammonium chloride, tetradecyl trimethyl ammonium chloride, cetyl trimethyl ammonium chloride, octadecyl trimethyl ammonium chloride, tetrabutyl ammonium bromide wholesale sales: dimethylolpropionic acid, DL-malic acid, isophthalic acid PIA, glycolic acid, adipic acid.

公司专业经营销售溴化物系列产品:溴化钠、溴酸钠、溴化钾、溴酸钾、四溴双酚A、十溴二苯醚、十溴联苯醚、十溴二苯乙烷、六溴环十二烷、二溴新戊二醇、三溴新戊醇、三溴苯酚、八溴醚、溴化聚苯乙烯、溴化铵、溴化钙、二溴丁二酸、二溴丁烯二醇、溴化表面活性剂系列:十二烷基三甲基溴化铵、十四烷基三甲基溴化铵、十六烷基三甲基溴化铵、十八烷基三甲基溴化铵、十二烷基三甲基氯化铵、十四烷基三甲基氯化铵、十六烷基三甲基氯化铵、十八烷基三甲基氯化铵、四丁基溴化铵批发销售:二羟甲基丙酸、DL-苹果酸、间苯二甲酸 PIA、羟基乙酸、己二酸。

To synthesize the octreotide, Phe-Leu-Glu-Glu-Leu and C_(15)H_(31)-CO-Lys-Thr -Thr-Lys-Ser peptides, the strategies are as followings: Fmoc- group as protective group for α-NH_2; TBTU, DIEA and HOBt as coupling reagent; and ethanedithiol -phenol - thioanisole - H_2O - TFA ( 2.5:5:5:5:82.5 , V/V ) as cleaving solution.

采用Fmoc固相合成法,以苯并三哗四甲基脲四氟硼酸盐、二异丙基乙胺和1-羟基苯骈三氮唑为缩合剂;苯酚—乙二硫醇—苯甲硫醚—水—三氟乙酸(5:5:2.5:5:82.5,V/V)为切割试剂,合成了奥曲肽、Phe-Leu-Glu-Glu-Leu及C_(15)H_(31)-CO-Lys-Thr-Thr-Lys-Ser三种小分子多肽,并对奥曲肽的合成规模进行了放大。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞,建立小鼠T淋巴细胞活化、增殖的模型,以不同浓度的CPT作用于该模型,流式细胞术检测T细胞早期活化标志CD69分子的表达;以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数;以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂,以不同浓度的TMB-8及与环孢菌素A联合作用于该系统,用流式细胞术检测T细胞早期活化标志CD69分子的表达;以活体染料羧基荧光素乙酰乙酸染色流式细胞术,分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数;以碘化丙啶染色分析细胞周期的分布情况。

Methods: The model of evaluating T cell proliferation stimulated with polyclonal activators, ConA was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. The effect of a specific inhibitor for p38 MAPK, SB253580, on T cell proliferation on different doses and in different time was estimated by flow cytometry. At the same time, the percentage of proliferating T cells and proliferation index were measured by CellQuest and SPSS10.0 for Windows softwares.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色,建立了在多克隆刺激剂刀豆蛋白A刺激下评价小鼠T细胞增殖的模型,通过流式细胞术分析p38丝裂原活化蛋白激酶的特异性抑制剂SB203580在不同剂量、不同时间对T细胞增殖的作用,并应用CellQuest和SPSS10.0 for Windows软件分析增殖细胞各代所占比例和增殖指数。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色,建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型,通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用;采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用;利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

College of Veterinary Medicine, China Agricultural University, Beijing 100094, China Abstract: A method has been developed to determine residual stilbenes such as diethylstilbestrol, dienestrol and hexestrol in animal tissues using solid phase extraction and gas chromatography-mass spectrometry.

动物组织样品在碱性条件下用乙酸乙酯提取,经碳酸钠溶液液-液分配净化,再用硅胶柱固相萃取净化,洗脱液均分成两份后经氮气吹干,分别用双三甲基硅基三氟乙酰氨和七氟丁酸酐衍生,采用选择离子监测模式进行测定,外标法定量。

Methods The effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 7-nitroindazole (7-NI) on nitric oxide synthase activity, levels of dopamine、3,4-dihydroxyphenylacetic acid、homovanillic acid, and TH-immunostained postive nerve fibres in the striatum of mice were determined by using colorimetic analysis, HPLC with electrochemical detection, and immunohistochemical analysis.

用比色分析、高效液相色谱电化学及免疫组化法检测1-甲基-4-苯基-四氢吡啶和7-硝基吲唑(7-NI)对C57BL小鼠纹状体一氧化氮合酶活性,多巴胺、二羟基苯乙酸、高香草酸水平和酪氨酸羟化酶免疫阳性神经纤维的影响。

Alanine, glycine and glutamine, and present weak resonances of creatinine also; diabetes mellitus patients show hyperglycorrhachia and strong resonances for ketone bodies and glycine; patients with benign prostatic hypertrophy or urethral stone have high levels of creatinine; the genital tumor females can be distinguished with low pyruvate levels on the bases of /sup 1/H-NMR spectra.

以下乃是由上述样品族群之氢光谱所得之结论;孕妇的脑脊髓液具有低浓度的脊髓糖;胺基酸(如丙氨酸;麸氨酸和甘氨酸)和肌酸酐;有酮体生成,糖尿病患的脊髓液在氢核光谱显示高浓度的脊髓糖,另外有丙酮酸;乙醯乙酸盐和甘氨酸的强烈共振讯号,而具摄护腺肥大症或是尿道结石的病患,具有高浓度的肌酸酐在其CSF的氢光谱中。

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