乙酸发酵

When the volumetric ratio of medium to flask was 0.20 and the corn steep liqud concentration was 8g/L, there have high yield of pyruvate and lactate produced at growth phase, 4.1g/L and 1.0g/L, respectively, which are ...

在装液比为 0 2和玉米浆浓度为 8g L时，丙酮酸和乳酸在细胞生长期可分别积累达 4 1g L和 1 0g L ，比正常发酵时增加 2倍以上，丙三醇产率也低；然而，装液比为 0 0 8和玉米浆浓度为 4g L时，丙酮酸和乳酸产生较低，丙三醇产率较高，但乙酸积累比供氧不足时高，可达 2 6g L。

One L-glutamic acid high-producing strain TN6319 with genetic maker (FEA~r+KMA~r+SG~r ) was screened through shake tube primary screening ,shake flask second screening,genetic marker test and single clone isolation and continuous passage cultibation.this strain could produce L-glutamic 72.9g/L after fermentation 42h under the fermentation without being optimized.

经摇管初筛、摇瓶复筛、遗传标记验证、单菌落分离和连续传代，最终筛选出一株L-谷氨酸高产菌TN6319(氟乙酸~r、酮基丙二酸~r、谷氨酰胺~r、磺胺胍~r)，该菌株在未优化条件下发酵42h产L-谷氨酸79.2g/L。

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatography mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80℃ and freeze-drying for four years. It could remain its degrading ability after 90℃ of high temperature treating for 30 min, and resume its degrading ability after subcultured 2 times after 105℃ of high temperature treating for 30 min in autoclave.

NSC-7在14 d的培养过程中，使稻秆分解73.6％；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸； NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30 min后，仍能够保持分解能力， 105℃处理30 min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6% of rice straw within 14 d. 10 kinds of material were detected in volatile products by gas chromatog- raphy mass spectrometry, and they were mainly acetic acid, glycerol, butyric acid, and propanoic acid.

NSC-7在14d的培养过程中，使稻秆分解73.6%；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸；NSC-7在-80℃冷冻和冻干条件下保存4年后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30min后，仍能够保持分解能力，105℃处理30min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

NSC-7 could degrade 73.6％of rice straw within 14d. 10 material were detected in volatile products by gas chromatography mass spectrometry,and it was mainly acetic acid, glycerol, butyric acid, and propanoic acid. NSC-7 could retain stable degrading ability and high cellulase activity after preserved in -80 and freeze℃-drying for four years. It could remain its degrading ability after 90 of high ℃temperature treating for 30min, and resume its degrading ability after subcultured 2 times after 105 ℃of high temperature treating for 30min in autoclave.

NSC-7在14d的培养过程中，使稻秆分解73.6％；用GC-MS测定结果发酵液中检测到10种化合物成分，其中峰值较大的依次为乙酸、甘油、丁酸、丙酸；NSC-7在-80℃冷冻和冻干条件下保存4a后仍具有稳定的秸秆分解能力和纤维素内切酶活性；经90℃高温处理30min后，仍能够保持分解能力，105℃处理30min后转接2次就能恢复分解能力，显示出很高的保存稳定性和热稳定性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。