乙酸乙酯

Methods The activitie of anti-lipidperoxidation of Chinemys Plastrum Testudinis of petroleum-ether, acetoacetate and 95% alcohol solution from two producing areas was studied by DPPH.

方法采用DPPH法分别测定两种不同产地龟板的石油醚、乙酸乙酯、95％乙醇等不同极性溶剂提取物的抗氧化活性。

Ointment extracted from Plastrum Testudinis was separated by silica gel column chromatography, gradiently eluted by petroleum ether-ethyl acetate, and 16 (Ts-1～Ts-16) components were obtained, which were biologically evaluated by MTT assay and flow cytometry on the proliferation of rat marrow-derived mesenchymal stem cells. Results showed that Ts-12 induced proliferation compared with the control group ( p ＜0.05), while Ts-4 showed inhibitive effect. Other groups showed no significance ( p ＞0.05). GC-MS was used to analyze the chemical components in different groups.

中药龟板提取物浸膏经硅胶柱层析，用石油醚-乙酸乙酯作洗脱剂，梯度洗脱，得到16个组分；采用MTT法和流式细胞技术研究了它们对鼠骨髓间充质干细胞的增殖作用，实验结果显示组分Ts-12具有促进rMSCs增殖的作用( p ＜0.05)，而组分Ts-4则表现出抑制rMSCs增殖的作用( p ＜0.05)，其它样品对rMSCs的增殖作用不显著( p ＞0.05)，不具统计学意义。

objective to observe the antitumor activities of different solution extracts of plumbago zeylanica l.in vitro.method mtt method was used to detect the inhibitory effects on four kinds of cancer cells.results chloroform extracts had significant inhibitory effects on the breast cancer cell(bre-04),the nerve cancer cell(n-04) and the lung cancer cell(lu-04),ic50 were 0.2699mg/ml and 0.2634mg/ml,0.4961mg/ml respectively.ic50 of chloroform extract on the hepg2 was 0.9379mg/ml.ic50 of petroleum ether extracts on bre-04,n-04,lu-04 and hepg2 was 0.5902mg/ml,0.5725mg/ml,0.7938mg/ml,0.6374mg/ml;ethyl acetate extracts had fairly inhibitory effects on the lu-04,ic50 was 0.7343mg/ml;n-butanol extracts and water-solubility extracts had notsignificant inhibitory effects on the four kinds of cancer cells.conclusion the antineoplastic effective parts of plumbago zeylanica l.were chloroform and petroleum ether extracts.

目的 探讨白花丹体外抗肿瘤作用的活性部位。方法 mtt染色法对白花丹五种溶剂提取物体外抗肿瘤活性进行了初步的研究。结果氯仿提取物对乳腺癌细胞（bre-04）、神经癌细胞（n-04）、肺癌细胞（lu-04）有较好的抑制生长作用，ic50分别为0.269 9 mg/ml、0.263 4 mg/ml、0.496 1 mg/ml，对肝癌细胞hepg2抑制作用差， ic50为0.937 9 mg/ml；白花丹石油醚提取部位对乳腺癌细胞（bre-04）、神经癌细胞（n-04）、肺癌细胞（lu-04）、肝癌细胞hepg2的ic50分别为0.590 2 mg/ml、0.572 5 mg/ml、0.793 8 mg/ml、0.637 4 mg/ml；乙酸乙酯提取物对肺癌细胞（lu-04）有一定的抑制作用，ic50为0.734 3 mg/ml；水溶性提取物以及正丁醇提取物无明显抑制肿瘤作用。结论白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好，值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

Said preparation method includes: dissolving curcumin and polyvinyl pyrrolidone according to mass ratio of 1:4-1:15 in organic solvent (for example absolute ethyl alcohol, propyl alcohol, isopropyl alcohol and ethyl acetate), making reduced pressure distillation at 55-95deg.C to remove most of ethyl alcohol to obtain paste material, quickly pouring the paste material into a stainless steel disk, vacuum drying at 60-90deg.C in drying oven and pulverizing.

本发明将姜黄素和聚乙烯吡咯烷酮按质量比1∶4～1∶15溶于有机溶剂(如无水乙醇、丙醇、异丙醇、乙酸乙酯等)，于减压蒸馏55-95℃下除去大部分乙醇至成糊状，迅速倒入不锈钢盘中，在60-90℃下真空烘箱烘干，用粉碎机粉碎，得到姜黄素固体分散体。

The effect of various polar fractions from Psoralea corylifolia L. ethanol extract on cell proliferation and differentiation were further examined and its ethyl acetate fraction were found to be active fraction.

对补骨脂不同极性萃取物进一步进行成骨样细胞增殖和分化活性测试，发现补骨脂乙酸乙酯萃取物为补骨脂中的主要活性部分。

Compared with OVX, the ethyl acetate fraction from Psoralea corylifolia L. significantly increased bone mineral density and bone calcium content of rats.

结果表明，给予补骨脂乙酸乙酯萃取物的大鼠与模型组大鼠相比，骨密度、骨钙含量显著增加。

The method to calculate the rate constant of the acetic ether saponification is in this way improved in this paper.

对乙酸乙酯皂化反应实验数据处理方法进行了改进，该方法无须测定初始电导，避免了初始电导的测定误差给计算结果带来的影响对两组实验数据进行了计算，结果表明，该方法简单、易操作，并有较高的精

Result: Ethyl acetate extract of sappanwood can evidently reduce the content of CD4~+ of T cell and the ratio of CD4~+/ CD8~+, moreover have no infection on the content of CD8~+ of T cell.

结果：苏木乙酸乙酯提取物组同空白对照组相比能明显降低CD4~+T细胞含量并降低CD4~+/CD8~+的比值，而对CD8~+T细胞的含量无影响。

Ethyl acetate extract of sappanwood can reduce the content of CD4~+ of T cell and the ratio of CD4~+/ CD8~+ of spleen,possess determinate function on immuno-supression.

苏木乙酸乙酯提取物能降低正常小鼠脾细胞CD4~+T细胞含量并降低CD4~+/CD8~+的比值，具有一定的免疫抑制作用。且无量效关系。

Ethyl acetate extract of sappanwood is dilued with olive oil.use the equivalent dosage of active compound sappanwood 35g, 45g, 55g/kg to intragastric administration;blank control group use 0.2ml/10g olive oil to intragastric administration.

苏木乙酸乙酯提取物用橄榄油稀释，以相当于原药苏木35g、45g、55g/kg的剂量灌胃；空白对照组灌服同体积的橄榄油。连续灌胃8天后处死。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。