乙酰化的

Objective Consequently, by constructing the pcDNA3-HA-hdacl, a HDAC1 eukaryotic expression vector, we were to study the interaction between HDAC1 and ERa and ERB in vitro and the effects of HDAC1 on the ERa and ERB at the protein level. So we can partly reveal the multiplication, differentiation and cancerization of lacteal gland cells.

目的 本文意在通过构建可在真核细胞中表达的重组质粒pcDNA3-HA-hdacl，研究组蛋白去乙酰化酶1(HDAC1)和雌激素受体ERα和ERβ在体外的相互结合作用，以及HDAC1对ERα和ERβ蛋白水平的影响规律，从而在一定角度揭示乳腺细胞的增殖、分化和癌变的过程，为更好地寻找治疗癌症的药物靶标打下基础。

We demonstrate that both the IL-5 mRNA expression and IL-5 protein production were stimulated by TSA and NaBu treatments. Chromatin immunoprecipitation assays showed that treatments of TSA and NaBu caused hyperacetylation of histones H3 and H4 at the IL-5 gene promoter in Jurkat cells, which consequently promoted the exogenous luciferase activity driven by this promoter.

TSA和丁酸钠能促进IL-5基因的mRNA和蛋白的表达水平，并增强该基因启动子所介导的外源荧光素酶报告基因的活性，这一增强作用与两种抑制剂介导的IL-5基因启动子上组蛋白H3和H4的高乙酰化有关。

Significant processing steps can include: a phase changes involving either the desired molecule or the solvent, inert carrier or vehicle, e.g., dissolution, crystallization, evaporation, sublimation, distillation or absorption; b a phase separation such as filtration or centrifugation; c any chemical change involving the desired molecule, e.g., removal or addition of water of hydration, acetylization, formation of the salt; d an adjustment of the solution containing the molecule such as adjustment of pH or pO2; e a precision measurement of contained or added BPC components, in-process solutions, recycled materials is performed, i.e., weighing, volumetric measuring, optical rotation, spectrophotometric determinations, etc.; and f changes occur in surface area, particle size, or lot uniformity, e.g., milling, agglomeration, blending.

意义较大的步骤可能会包括：a相变化，包括指定分子或溶剂，惰性载体或溶媒，如溶出作用，结晶，蒸发，升华，蒸馏或吸附；b相分离，如过滤或离心；c涉及指定分子的任一化学变化，如结晶水的除去或添加，乙酰化作用，成盐；d包含分子的溶解的调节，如调节pH或pO2；e 含有或添加原料药组分，过程内溶液，回收物料的精密测量，如，称重，体积测量，旋光度，分光光度测定，等和f 表面，粒度，或批一致性的变化，如磨碎，凝块，混合。

Formyl-3-acetyl-4-methyl-5-carbobenzoxypyrrole (6) was synthesized via the selective oxidation of 2,4-dimethyl-3-acetyl-5-carbobenzoxypyrrole (5), which was produced from acetylacetic benzylester (1) via nitrosation, reduction and addition-cyclization with acetylacetone. The total yield of the product was about 34%.

以乙酰乙酸苄酯(1)为原料，经过亚硝化、还原、与乙酰丙酮加成环化生成2， 4-二甲基－3-乙酰基－5-苄氧羰基吡咯(5)，通过选择性氧化反应制得目标化合物2-甲酰基－3-乙酰基－4-甲基－5-苄氧羰基吡咯(6)，反应的总收率约为34%。

Firstly, 3-ethylthiobutanal was prepared from crotonaldehyde reacting with ethanehiol in triethylamine with high yield. Secondly, methyl acetoacetate was converted to 6-ethylthiohept-3-en-2-one by three steps via hydrolysis, condensation with 3-ethyltio-butanal and dehydration, then 6-ethylthiohept-3-en-2-one was cycloco ndensed with dimethyl malonate by Michael addition and Claisen condensation, followed by saponification and decarboxylation to give the intermediate of 5-(2-ethylthiopropyl)-cyclohexane-1,3-dione.

首先，以巴豆醛与乙硫醇为原料，在三乙胺的催化作用下高收率制得3-乙硫基丁醛；然后从乙酰乙酸甲酯出发，通过碱水解、与3-乙硫基丁醛缩合、脱水三步反应合成6-乙硫基-3-烯-2-庚酮，接着6-乙硫基-3-烯-2-庚酮与丙二酸二甲酯通过Michael加成和Claisen缩合成环，再通过皂化和脱羧反应生成中间体5-[2-丙基]-1，3-环己二酮；再与丙酸酐反应丙酰化，最后与0-3-氯-2-丙烯基羟胺肟化，得到目标化合物烯草酮。

The optimum reaction condition of NaClO oxidative pretreating and viny acetate acetylating technique has been determined according to sizing property, that further improves the function of acetylated starch size and expands its application in warp sizing .

对醋酸淀粉酯的变性工艺进行了系统的研究和探讨，以浆纱性能为依据，确定了次氯酸钠氧化预处理工艺和醋酸乙烯酯乙酰化工艺的最佳反应条件，进一步提高了醋酸淀粉酯浆料的性能，扩大了醋酸淀粉酯在纺织经纱上浆中的应用

The quantity of acetylated starch was 35%, the quantity of cross-linked starch was 3%,the quantity of cellulose was 1.5%, the quantity of honey was 60% in the power, the water content of the sample before drying was 35%, the dryer temperature was 135～140℃ and the dried t...

对采用滚筒干燥生产固体蜂蜜的配方及其工艺参数进行了实验研究，初步确定了配方和工艺条件为干粉中乙酰化淀粉的用量35%、交联淀粉的用量3%，纤维素的用量1.5%，蜂蜜的含量60%，干燥前样品的水分含量35%，干燥温度135～140℃，干燥时间90s。

The results suggested that chitinhad a good solubility in the new solvent when its at -30℃and below, but the solubilityof chitin no longer increasesd notable as the temperature reduced; The joined of urea, incertain degree, strengthened the solvency of alkali solvent to chitin. When theconcentration of sodium hydroxide is 8%～12%, Chitin had a good solubility, and theapparent viscosity of chitin solution could up to 4000 mpa·s～5000mpa·s at roomtemperature; In addition,the solubility of chitin in new solvent was high when therefrigerated time was three days and above, but the refrigerated time could not too long,otherwise, the deacetylize degree of chitin got more bigger. The properties of chitin solution were tested by revolving viscosimeter, FT-IR,X-RD and so on.

结果表明，在-30℃及以下甲壳素能很好的溶解于新溶剂中，但甲壳素的溶解度不再随温度的下降而有所增大，而是基本保持不变；尿素的加入，在一定程度上强化了强碱溶剂对甲壳素的溶解，当新溶剂中NaOH浓度为8％～12％时，甲壳素在试验温度下可以获得较好的溶解度，所得溶液在室温下的表观黏度达4000 mpa·s～5000mp·s；冷冻时间为3 d及以上时，有利于甲壳素的溶解，超过3 d，甲壳素的溶解度变化不大，另外，冷冻条件下，甲壳素在强碱溶剂中仍会发生脱乙酰化反应，冷冻时间不宜太长。

These data suggest that histone acetylation during nuclearremodeling (from the start of activation to the formation of pronuclei) is independent fromthe event that oocyte cytoplasm inhibits the transcription of specific genes in thedifferentiated cells, but induces reprogramming of gene expression.

这表明组蛋白的乙酰化修饰可能没有参与卵细胞质对分化细胞特有的基因转录的抑制，但引起了基因表达模式的变化。

Meanwhile,HDAC1 acetylation level is significantly increased.The overexpression of HDAC1 can promote erythroid cell proliferation and inhibits induced differentiation,the knockdown of HDAC1 or 2 inhibits erythroid cell proliferation and promotes induced differentiation.We also found that HDAC1 affects GATA1 mediated transcription activity.Thus our data revealed that HDAC1 may negatively regulate GATA1 mediated transcription and suggest that the dynamic regulation of GATA1 associated NuRD/MeCP1/HDAC1/HDAC2 complex may determine the onset of erythroid differentiation programs.Moreover,this study will eventually help to design new therapeutic approaches in treatment of leukemia.

研究发现在被诱导分化的小鼠红细胞白血病细胞中与GATA1相结合的HDAC1被乙酰化并失去活性，在血细胞中过表达HDAC1能够促进血细胞的增殖并抑制分化，反之在血细胞中敲除HDAC1或HDAC2能抑制血红细胞的增殖并促进分化，而且HDAC1能够负调控GATA1的转录活性，GATA1与NuRD/MeCP1复合体之间的动态调控，决定血红细胞分化的发生和发展，并为探索白血病的致病机理奠定了理论基础。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。