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Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷(5-Aza-2'-dc,以下简称Aza)及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后,用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况,用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况,并用逆转录聚合酶链反应检测ING1bmRNA表达。

The improved method of konjac glucomannan carboxy methylation was proposed in this paper according to the effects of deacetylation. Carboxymethyl KGM were synthesized by blending KGM with etherification agent first and then basifying and catalyzing in ethanol. Basifying first and then etherifying prepared traditional carboxymethyl KGM.

针对脱乙酰基的这种影响进行羧甲基化方法的改进,在乙醇介质中先混合醚化剂,再进行碱化催化反应制备羧甲基魔芋葡甘聚糖,并通过与传统的先碱化再醚化的KGM羧甲基改性方法比较,达到乙酰基脱出顺序上的不同。

Therefore, the author excogitates some synthesis courses of new compounds including selenium and aspirin based on medicament chemistry and coalition principle. The new compound is named 5-5'-diselenium monoacetyl salicylic acid and ascertained by IR ,UV and MALDI-TOF-MS, and its structure is: OOHThe compound is inhibiting to sarcoma and ascites tumour of white mice by pharmacology experiments, so it is worth researching deeply.

为此,本文运用化学拼合原理,提出了几种既含硒又含乙酰水杨酸的化合物新结构的合成路线,经过多次理论分析和探索试验,得到一种预测的新的化合物,命名为5-5′-二硒单乙酰水杨酸,经红外、紫外、飞行时间质谱测定,证明该化合物的结构为:经药理预试验表明,该化合物对小鼠S_(180)肉瘤和艾氏腹水型肿瘤均有抑制作用,具有深入研究的意义。

The similar nicotinoyltrifluoroacetones are synthesized from acetylpyridine and ethyl trifluoroacetate through Claisen reaction.The influnences of alcoholic natrium,reaction temperature and solvent on reaction yield is studied.The subliming method is improved.

本文以乙酰吡啶和三氟乙酸乙酯为主要原料,利用克莱森酯缩合反应,合成了三种相似结构的吡啶甲酰三氟丙酮化合物,考察了碱性试剂醇钠、反应温度和溶剂等因素对反应产率的影响,改进了纯化产物的方法。

Could keep above 95% in the fixed beds. In order to achieve high stereo-selectivity with yeast, ethyl acetoacetate was used as the model substrate,and a novel approach was proposed to selectively inhibit R-enzymes in yeast before reaction.

乙酰乙酸乙酯为模型底物,以丙烯酸、乙醚、二甲基亚砜、丙烯酰胺、正己烷等有机溶剂选择性地抑制酵母中R型酶,提高反应立体选择性。

The exchange and adsorption properties of LA, FA and glu on six anion exchange resins were investigated, and the influence of the temperature was also researched, among them weakly basic ion-exchange resin 335 can provide the highest uptake capacity of LA The Freundlich equation can explain the adsorption equilibrium curves of LA on the anion resins better than the Langmuir equation.

在静态吸附性能研究部分,研究了乙酰丙酸、葡萄糖和甲酸在六种阴离子树脂上的静态吸附行为,筛选出吸附性能最好的335树脂,Freundlich方程比 Langmuir方程能更好的描述树脂对乙酰丙酸和甲酸的吸附。

Low molecular weight chitosan is prepared from breakdown of glycosidic bond of chitosan.

甲壳素是N-乙酰葡萄糖胺的聚合物,甲壳素脱乙酰后为壳聚糖,壳聚糖糖苷键断裂降解为低分子量壳聚糖。

The results showed 3 types of aluminum compound prepared in this paper all had good poison protection effectiveness on such impurities as stannous chloride,lead oxide,sodium hydrogen sulfate,and so on.Moreover,the mechanism of the poison of catalyst was also discussed.

结果表明,合成的3种防中毒剂即防-1、防-2(主要成分为乙酰丙酮合铝)及防-3(主要成分为二乙酰丙酮基异丙氧基铝)对二月桂酸二丁基锡、氯化亚锡、二正丁胺、硫脲、氧化铅及硫酸氢钠等杂质均有较好的防中毒作用;不同防中毒剂对不同杂质的防中毒作用有一定的选择性。

The effect of chloroacetyl chloride and bromoacetyl bromide on yields of intermediates and nitrazepam was compared.

比较了氯乙酰氯和溴乙酰溴对中间体及硝基安定收率的影响。

Results:paracetamol injection solution of 25% was produced through the study on technology.

结果:本实验通过对乙酰氨基酚的工艺研究生产出了25%的对乙酰氨基酚注射剂。

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