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Keywords diethyl carbonate;phenol;phenetole;potassium carbonate;active carbon;ethylation;solid base catalyst

碳酸二乙酯;苯酚;苯乙醚;碳酸钾;活性炭;乙基化;固体碱催化剂

Phototaxis insects, ethyl acetate, collection methods

中文关键字:趋光性昆虫,乙酸乙酯,收集方法

Methods The activitie of anti-lipidperoxidation of Chinemys Plastrum Testudinis of petroleum-ether, acetoacetate and 95% alcohol solution from two producing areas was studied by DPPH.

方法采用DPPH法分别测定两种不同产地龟板的石油醚、乙酸乙酯、95%乙醇等不同极性溶剂提取物的抗氧化活性。

Ointment extracted from Plastrum Testudinis was separated by silica gel column chromatography, gradiently eluted by petroleum ether-ethyl acetate, and 16 (Ts-1~Ts-16) components were obtained, which were biologically evaluated by MTT assay and flow cytometry on the proliferation of rat marrow-derived mesenchymal stem cells. Results showed that Ts-12 induced proliferation compared with the control group ( p <0.05), while Ts-4 showed inhibitive effect. Other groups showed no significance ( p >0.05). GC-MS was used to analyze the chemical components in different groups.

中药龟板提取物浸膏经硅胶柱层析,用石油醚-乙酸乙酯作洗脱剂,梯度洗脱,得到16个组分;采用MTT法和流式细胞技术研究了它们对鼠骨髓间充质干细胞的增殖作用,实验结果显示组分Ts-12具有促进rMSCs增殖的作用( p <0.05),而组分Ts-4则表现出抑制rMSCs增殖的作用( p <0.05),其它样品对rMSCs的增殖作用不显著( p >0.05),不具统计学意义。

objective to observe the antitumor activities of different solution extracts of plumbago zeylanica l.in vitro.method mtt method was used to detect the inhibitory effects on four kinds of cancer cells.results chloroform extracts had significant inhibitory effects on the breast cancer cell(bre-04),the nerve cancer cell(n-04) and the lung cancer cell(lu-04),ic50 were 0.2699mg/ml and 0.2634mg/ml,0.4961mg/ml respectively.ic50 of chloroform extract on the hepg2 was 0.9379mg/ml.ic50 of petroleum ether extracts on bre-04,n-04,lu-04 and hepg2 was 0.5902mg/ml,0.5725mg/ml,0.7938mg/ml,0.6374mg/ml;ethyl acetate extracts had fairly inhibitory effects on the lu-04,ic50 was 0.7343mg/ml;n-butanol extracts and water-solubility extracts had notsignificant inhibitory effects on the four kinds of cancer cells.conclusion the antineoplastic effective parts of plumbago zeylanica l.were chloroform and petroleum ether extracts.

目的 探讨白花丹体外抗肿瘤作用的活性部位。方法 mtt染色法对白花丹五种溶剂提取物体外抗肿瘤活性进行了初步的研究。结果氯仿提取物对乳腺癌细胞(bre-04)、神经癌细胞(n-04)、肺癌细胞(lu-04)有较好的抑制生长作用,ic50分别为0.269 9 mg/ml、0.263 4 mg/ml、0.496 1 mg/ml,对肝癌细胞hepg2抑制作用差, ic50为0.937 9 mg/ml;白花丹石油醚提取部位对乳腺癌细胞(bre-04)、神经癌细胞(n-04)、肺癌细胞(lu-04)、肝癌细胞hepg2的ic50分别为0.590 2 mg/ml、0.572 5 mg/ml、0.793 8 mg/ml、0.637 4 mg/ml;乙酸乙酯提取物对肺癌细胞(lu-04)有一定的抑制作用,ic50为0.734 3 mg/ml;水溶性提取物以及正丁醇提取物无明显抑制肿瘤作用。结论白花丹氯仿提取部位、石油醚提取部位体外抗肿瘤作用较好,值得对其提取部位进一步分离纯化并研究其抗肿瘤作用机制。

Objective To observe the applied effects of combining urethane with procaine in experimental anaesthesia of rabbits.

目的 观察氨基甲酸乙酯与普鲁卡因联合应用在实验兔中的麻醉效果。

The result was shown as follows:compared with the good quality beer,the diacetyl,aldehyde,islobutyl alcohol,isoamyl alcohol and total acid were higher,however,propyl alcohol and ethyl butyrate were lower

研究中还发现污染细菌后的发酵过程中的pH下降迅速,降糖加快,最终得到的啤酒中双乙酰、乙醛、异戊醇、异丁醇和总酸偏高,而正丙醇、丁酸乙酯含量偏低。

Said preparation method includes: dissolving curcumin and polyvinyl pyrrolidone according to mass ratio of 1:4-1:15 in organic solvent (for example absolute ethyl alcohol, propyl alcohol, isopropyl alcohol and ethyl acetate), making reduced pressure distillation at 55-95deg.C to remove most of ethyl alcohol to obtain paste material, quickly pouring the paste material into a stainless steel disk, vacuum drying at 60-90deg.C in drying oven and pulverizing.

本发明将姜黄素和聚乙烯吡咯烷酮按质量比1∶4~1∶15溶于有机溶剂(如无水乙醇、丙醇、异丙醇、乙酸乙酯等),于减压蒸馏55-95℃下除去大部分乙醇至成糊状,迅速倒入不锈钢盘中,在60-90℃下真空烘箱烘干,用粉碎机粉碎,得到姜黄素固体分散体。

The effect of various polar fractions from Psoralea corylifolia L. ethanol extract on cell proliferation and differentiation were further examined and its ethyl acetate fraction were found to be active fraction.

对补骨脂不同极性萃取物进一步进行成骨样细胞增殖和分化活性测试,发现补骨脂乙酸乙酯萃取物为补骨脂中的主要活性部分。

Compared with OVX, the ethyl acetate fraction from Psoralea corylifolia L. significantly increased bone mineral density and bone calcium content of rats.

结果表明,给予补骨脂乙酸乙酯萃取物的大鼠与模型组大鼠相比,骨密度、骨钙含量显著增加。

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