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Amino-6-methoxy Benzothiazole 99%、2-Amino Benzothiazole 99%、Veratraldehyde 99%、Veratric Acid 99%、1,2- Dimethoxy Benzene 99%、6-Nitroveratric Acid 97%、2,5-Dichlorobenzoic Acid 99%、4-Chloro-3-Nitrobenzoic Acid 99%、 3-Nitrobenzole Acid 98%、p-Methoxybenzoie Acid 99%、Anisole 99.5%、p-Methoxyacetophenonc 99%、(2'-Chloroethyl) Benzene 99%、(2'-Bromoethyl) Benzene 99%、N,N-Diethyi-m-Toiuamide 99%、Aminoacetonitrile Hydrochloride 98%、 Alkyl Diphenyl Phosphate Estrel 98%、5-Chloro-2-benzothiazolone 99%、6-bromo-2-naphthol 98%、6-Nitro-Veretric Acid Estrel 97%、4-chloro phenoxyaeetic 99

2-氨基-6-甲氧基苯骈噻唑、2-氨基苯骈噻唑、藜芦醛、藜芦酸、藜芦醚、6-硝基藜芦酸、2,5-二氯苯甲酸、3-硝基-4-氯苯甲酸、3-硝基苯甲酸、对甲氧基苯甲酸、苯甲醚、对甲氧基苯乙酮、β-氯代苯乙烷、β-溴代苯乙烷、N,N-二乙基间甲苯甲酰胺、氨基乙腈盐酸盐、磷酸二苯月桂酯、5-氯-2-苯骈噻唑酮、6-溴-2-萘酚、6-硝基藜芦酸甲酯、4-氯苯氧乙酸

Methods A mixture of 10 mol/L potassium dihydrogen phosphate solution and acetonitrile(60 ∶ 40) was used as the mobile phase.

方法以乙腈-10mmol/L磷酸二氢钾(40∶60)为流动相,炔雌醇为内标,血浆样品经乙酸乙酯萃取。

The process was methanolysis α-imino ether acid hydrogen chloride salt from methyl-cyanide.

研究了以乙腈为原料经α-亚氨基乙醚盐酸盐醇解制备原醋酸二乙酯的工艺方法,对醇解的投料比、反应时间及中和pH值等工艺条件进行了研究,得出较优惠的工艺条件。

The linear ranges of the calibration curves for lycorine,lycoramine and galanthamine were 5.0-400 mg/L,respectively.

采用三乙胺水溶液(pH 9.0)-乙腈-甲醇作为流动相对石蒜碱、力可拉敏和加兰他敏进行HPLC测定,方法的线性范围为5.0~400 mg/L,检出限分别为5.0、5.1、10.2 ng,RSD分别为2.2%、5.7%和4.4%。

The linear ranges of the calibration curves for lycorine, lycoramine and galanthamine were 5.0-400 mg/L, respectively. Their detection limits were 5.0, 5.1 and 10.2 ng, respectively and the RSD were 2.2%, 5.7% and 4.4%, respectively.

采用三乙胺水溶液(pH9.0)-乙腈-甲醇作为流动相对石蒜碱、力可拉敏和加兰他敏进行HPLC测定,方法的线性范围为5.0~400mg/L,检出限分别为5.0、5.1、10.2ng, RSD分别为2.2%、5.7%和4.4%。

The chromatographic conditions were as followed: C8 column, 0.045 mol/L ammonium phosphate monobasic solution∶acetonitrile (2∶1)(adjusted to pH7.35 with triethylamine) as mobile phase, temperature: 40℃, the flow rate: 1.0 ml/min, detection wavelength: 210nm.

采用C8柱,以0.045mol/L磷酸二氢胺溶液∶乙腈(2∶1)(用三乙胺调pH至7.35)为流动相,流速1ml/min,检测波长210nm,柱温40℃。

The chromatographic conditions were as followed: C8 column, 0.045 mol/L ammonium phosphate monobasic solution∶acetonitrile (2∶1)(adjusted to pH7.35 with triethylamine) as mobile phase, temperature: 40℃, the flow rate: 1.0 ml/min, detection wavelength: 210nm.

采用C8柱,以0.045mol/L磷酸二氢胺溶液∶乙腈(2∶1)(用三乙胺调pH至7.35)为流动相,流速1ml/min,检测波长210nm,柱温40℃。结果阿奇霉素的线性范围为50.3~1006μg/ml,平均回收率100.1%,r=1.0000,RSD=0.8%(n=9)。

Hypersil of ODS-C18 was used with acetonitrile-0.01mol/L monobasic potassium phosphate-triethylamine phosphoric acid8:92:0.4:0.3) as mobile phase.

色谱柱:依利特 Hypersil ODS柱4.6 mm×250mm,5μm),流动相:乙腈-0.01mol/L磷酸二氢钾溶液-三乙胺-磷酸(8 : 92 : 0.4 : 0.3)。

The chromatographic conditions were as followed: C8 column, 0.045 mol/L ammonium phosphate monobasic solution∶acetonitrile (2∶1)(adjusted to pH7.35 with triethylamine) as mobile phase, temperature: 40℃, the flow rate: 1.0 ml/min, detection wavelength: 210nm.

采用C8柱,以0.045mol/L磷酸二氢胺溶液∶乙腈(2∶1)(用三乙胺调pH至7.35)为流动相,流速1ml/min,检测波长210nm,柱温40℃。结果阿奇霉素的线范围为50.3~1006μg/ml,平均回收率100.1%,r=1.0000,RSD=0.8%(n=9)。

Methods The HPLC System consisted of Agilent Plus C18 column, acetonitrile-buffer(0.01mol · L-1 monobasic potassium phosphate, contained 0.1% triethyl amine, pH 6.8 )(70∶30) as mobile phase with detection wavelength 282nm.

采用 Agilent Plus C18柱;以0.01mol · L-1磷酸二氢钾溶液(含0.1%三乙胺,用磷酸调节 pH 值至6.8)-乙腈(30∶70)为流动相;检测波长:282nm 。

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