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Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例,分成三份,分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本,用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA,以RT-PCR方法扩增β-Actin基因,经1.5%琼脂糖凝胶电泳,溴化乙锭染色,紫外透射仪下观察分析。

The results show that 60Co-γ ray radiation has the most remarkable effect on the degradation of organophosphorus pesticides such as fenitrothion, parathion, methyl-parathion, phorate and chlorpyrifos and the maximum degradation rates are over 85%. The degradation rates of methamidophos, acephate, malathion ,dimethoate are slightly inferior to them and the optimum degradation rates is just only 34.70%. At the same time, the contents of soluble total sugars, glucose, fructose, sucrose and total sugars in apple juice decrease unconspicuously, but the total acid content in apple juice and its color value and clarity increase. Based on a comprehensive consideration of the 60Co-γ radiation effect on the actual degradation of organophosphorus pesticides in apple juice and its main chemical and physical indexes, organophosphorous pesticides residue will be decreased to a lower level with radiation dose of about 5 kGy but the main chemical and physical indexes of export apple juice can not be affected.

结果表明: 60Co-γ射线辐照处理对苹果汁中杀螟硫磷、对硫磷、甲基对硫磷、甲拌磷、毒死蜱降解效果最为显著,最高降解率均在85%以上;对苹果汁中甲胺磷、乙酰甲胺磷、马拉硫磷和乐果的降解效果不显著,最高降解率仅为35%;在0~9 kGy辐照剂量范围内降解苹果汁中有机磷农药的同时,会使苹果汁中的可溶性固形物、葡萄糖、果糖、蔗糖及总糖含量轻微降低,苹果汁的总酸度有所增加,苹果汁的色值及透光率提高;综合分析60Co-γ射线辐照对苹果汁中有机磷农药的实际降解效果及对苹果汁主要理化指标的影响,选用5 kGy的辐照剂量已完全使苹果汁中的有机磷农药残留降低到一个很低的水平,又不会对苹果汁出口主要理化指标造成影响。

Methods n-Cetyl-l-thio-β-D-galactoside was synthesized by a series of reaction from galactose and incorporated into Small Unilamellar Vesicles which was prepared by sonication at the ratio for L-α-PC∶Chol∶L-α-PE as 7∶2∶1, with or without 10 mol%Cetyl-gal.

半乳糖经乙酰化、溴化、缩合、亲核取代反应制备半乳糖新糖脂质并与其他脂质制备脂质体,在二氯化锡还原作用下用99mTc标记脂质体,进行动物体内靶肝实验,用γ计数器检测各脏器放射性,计算摄取率。

In anaerobic conditions,yest and other organisms carry out alcoholic fermentation that converts pyruvate to acetaldehyde and then to ethanol, regenerating NAD+ that allows glycolysis to continue.

在缺氧条件下,酵母和其他组织进行醇的发酵,使丙酮酸转化为乙全然后为乙醇,再生NAD+,使糖酵解继续进行。

In the chloralose-urethanized or curarized rabbits,infusion of saline or blood into the right auricle in amount of 8—10 ml/kg at the rate of 0.5—1.0 ml/sec induced bradycardia and hypotension.

一在氯醛糖和氨基甲酸乙酯麻醉的兔或未麻醉的箭毒化兔,右心房内输液(输入量为8—10毫升/公斤,输入速度为0.5—1.0毫升/秒),可规律地引起心率减慢和动脉血压下降。

Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.

用20%聚乙二醇(polyethylene glycol,PEG6000)模拟干旱,采取CTAB提取缓冲液,按一定比例加入PVP和β-巯基乙醇,用Tris平衡酚-氯仿-异戊醇除去蛋白质及杂质,在粗提时用异丙醇沉淀DNA,并用DNA洗液(10mmol/L醋酸铵,75%乙醇)洗涤DNA,在纯化时用乙醇沉淀DNA,且没有加入RNase去除RNA的方法,分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA,并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。

The main kinds of the "SongXue Brand" are high-transformation type amylum saccharified enzyme,high-temperature resistant α-amylase,BAA- middle temperature resistant amylase ,pepsin,cellulase,feed complex enzyme and alcohol instant active dry yeast.

主要品种有&松雪&牌:高转化率糖化酶、耐高温α-淀粉酶、BAA-中温淀粉酶、酸性蛋白酶、纤维素酶、饲料复合酶和酒用活性干酵母、&天科&牌α-乙酰乳酸脱羧酶。

The results showed that Kaidimycin was chemically neutral, macrocylic polylactones, stable and liable to dissolve into water and pyridine, slight to dissolve into methanol, ethanol and N-butanol, difficult to dissolve into ethyl acetate, acetone, benzene and chloroform. Chemical reaction of functional group of Kaidimycin showed that it had glycoside, amino acid and aldehyde, no reducing sugar, polypeptide and phenol.

凯地菌素属中等极性的、中性抗生素,极性纸层析结果与多烯类抗生素的纸层析结果相似:易溶于水、吡啶,微溶于甲醇、乙醇、正丁醇,不溶于乙酸乙酯、丙酮、苯、三氯化碳;对高温、光照和紫外处理有很好的稳定性;官能团反应试验结果发现,凯地菌素可能含有糖苷键、氨基酸伯氨基和醛基,不含有还原糖、多肽和酚基,高锰酸钾、浓硫酸反应与多烯大环内酯类抗生素的特性相似;并且依据纯品,采用生物法和分光光度法对摇床发酵液进行了效价的测定。

Which can be used as the body of vitamin B1 coenzyme of many enzymes in the normal human body has an important role in glucose metabolism, it can inhibit the activity of cholinesterase, and choline esterase is an important neurotransmitter, and thus nerve conduction impact; vitamin B2 in the organization by participating in a variety of yellow enzymes constitute a coenzyme, its role in bio-oxidation process in the delivery of hydrogen role in regulating the body's metabolism process; vitamin B6 can also be a variety of enzymes as coenzyme participate in all kinds of amino acid metabolism, promote the absorption of amino acids and protein synthesis, cell growth is necessary for the material, which also promote fat metabolism, involved in neurotransmitter synthesis, hemoglobin synthesis and the impact on the normal reproductive functions such as human body an important metabolic process, Volkswagen Medicine Net: What liver protection effect of vitamin B family?

可作为维生素乙许多酶的辅酶,在正常人体组织中有一个用糖代谢的重要作用,能抑制胆碱酯酶活性,和胆碱脂酶是一个重要的神经递质,神经传导,从而影响;由黄色参与各种酶在组织中维生素B2构成辅酶,其作用在生物氧化氢的作用,调节人体的代谢过程中产程;维生素B6也可以是作为辅酶参与氨基酸代谢的各种酶的品种,促进氨基酸的吸收和蛋白质合成,细胞生长是必要的材料,这也促进脂肪代谢的神经递质合成,血红蛋白合成和参与,如影响人体生殖功能的正常新陈代谢过程中一个重要的,(大众医药网:什么样的维生素B家庭肝脏的保护作用?

MethodsSixty Wistar rats were divided into 2 groups randomly: normol group(n=12), and model group(n=48).The model rats were fed with high fat-sugar diet for 6 weeks, then fed with adenine(100 mg·kg-1·d-1) and ethambutol(250 mg·kg-1·d-1) for 2 weeks to induce the IR and hyperuricemia model.

方法选用 60只成年Wistar雄性大鼠,随机分成正常对照组(12只)和模型组(48只),模型组给予高脂高糖饲料餵养6周,6周后再用腺嘌呤〔100 mg/kg·d)〕加乙胺丁醇〔250 mg/〕灌胃2周,造成胰岛素抵抗并高尿酸血症的大鼠模型。

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