主要的人

Since the collapse of the currency in October and amid a banking crisis that has saddled many Icelanders with unpayable debts (and cost many foreigners their savings), thousands of people, mainly left-wingers, had gathered each week to vent their anger.

自从十月份爆发货币崩溃并伴随着使许多冰岛人背上无法偿付的债务（并赔上了许多外国人的积蓄）的银行危机后，数以千计的人，主要是左翼份子，每周都会聚集起来发泄他们的不满。

The accounting information system uses computer as main tool, by which users can collect, store and analyze variety of accounting data and provides man-machine system of accounting information for the user.

会计信息系统是以计算机为主要工具，对各种会计数据进行收集、存储及分析，为用户提供会计信息的人——机系统。

City standard for many people, money is the main source of water.

城标对很多人来说，是主要的水钱来源。

Experts believe women are naturally programmed to be the weaker sex when it comes to fighting off HIV.

我想这主要是针对随手乱丢垃圾的人，那样不仅污染环境，还造成浪费。

There are mainly three kinds of race s in the world; the white race , the black race and the yellow race .

世界上有三个主要的种族：白种人，黑种人和黄种人。

There are mainly three kinds of races in the world; the white race, the black race the yellow race.

世界上有三个主要的种族：白种人，黑种人和黄种人。

BackgroundSchistosomiasis japonicum remains to be a serious zoonosis and mostly prevalent in developing country.

研究背景日本血吸虫病是一种危害严重的人兽共患寄生虫病，主要流行于发展中国家。

Objective To study the efficacy of prophylactic therapy of HCMV disease for patients underwent cadaveric renal transplantation.

人巨细胞病毒感染是肾移植受者术后最主要的合并症和死亡原因之一，其感染发生率约为70%，其中10%～30%可发展成HCMV病［1，2］。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The Mitanni were an eastern Indo-European people (belonging to the linguistic "satem" group) who settled in northern Mesopotamia circa 1600 BC South-East of Turkey and by circa 1450 BC established a medium-size empire east, north and west, and temporarily made tributary vassals out of kings in the west, even as far as Kafti and making them a major threat for the Pharaoh.

米坦尼人是一群东部印欧语系的人（属于语言学上的&S类语言&组别），大约在公元前1600年从土耳其东南部移居到美索不达米亚，大约在公元前1450年建立一个中等大小的帝国，让东面、北面和西面，甚至远至卡夫提的附庸诸候国暂时脱离了西面的国王，使他们成为了埃及法老的主要威胁。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。