主动脉周的
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Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.
采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型,分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本,行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况;行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润;行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达;分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。
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Methods Twenty-six NZW rabbits were randomly divided into three groups: a normal control group (fed on normal commercial rabbit diet, n =6), a hyperlipidemic group fed on high-fat ma total cholesterol (TC concentration at the beginning and the 12-week end point of the experiment determined,and all the experimental rabbits were sacrificed, and endothelial function test were performed on thoracic aortic rings prepared from the isolated aorta using the vascular endothelium-dependent relaxation parameter in response to acetylcholine The aortic atherosclerotic lesion expression of LOX-1 mRNA and protein were examined by RTPCR and Western blotting respectively.
方法新西兰大白兔随机分为正常对照组(普通饮食,6只),高脂饮食组(高脂饮食,10只)及普罗布考组(高脂饮食+普罗布考200mg/kg·d,10只)。实验开始前及第12周分别耳缘静脉采血测定血清总胆固醇。第12周处死动物,取胸主动脉,制备离体胸主动脉环对乙酰胆碱的反应以检查内皮功能,RT-PCR与免疫印迹检测凝集素样氧化低密度脂蛋白受体-1(LOX-1)基因与蛋白质表达水平。
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Methods and Results— Thoracic aortic segments from 8-week-old female B6 and C3H apolipoprotein E–deficient mice were transplanted into the infrarenal aorta of 10-week-old female F1 mice.
方法和背景:将8周大雌性B6和C3H载脂蛋白E缺陷老鼠的胸主动脉节段移植到10周大雌性F1老鼠的肾下主动脉。
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Methods Rabbits were randomized to three groups. Complex model group were fed with feed containing 0.5% cholesterol for 3 weeks. At the fourth, eighth and twelfth weeks, rabbits were given injection of lipopolysaccharide (1 μg) at auricular artery, cervical part and fold inguen, respectively. Normal group were fed with normal diet, while High cholesterol group were fed with feed confaining 0.5% cholesterol only. After 16 weeks, vascular tissues were analyzed using morphometric and histological methods, and the sera were checked for cholesterol, LDL-C, C-reaction protein and tumor necrosis factor-α. Results The cholesterol and LDL-C of High cholesterol group were increased, compared to those of normal group.
给含0.5%胆固醇的饲料,3周后,分别在第4、8、12周采用耳动脉内、颈部、腹股沟处肌肉注射大肠埃希菌脂多糖,并设立正常组和单纯高脂组。16周后观察兔的一般状态,取血清检查血脂六项、C-反应蛋白和TNF-α,取耳动脉、颈动脉、主动脉弓、胸主动脉、腹主动脉、髂动脉、肝脏,放置4%多聚甲醛中过夜,常规行HE染色,检查血管病变和相关脏器病变情况。
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Then animals were killed by gas embolism, aorta was separated from the beginning of aortic arch to abdominal aorta crotch. At the end of experiment, the aortas were prepared for measuration of the concentration of ceramide and cholesterol. The pathological changes and plaque area were measured.
第十周实验结束时,用气栓法处死动物,将主动脉全部取下,沿正中线剪开,摄像,同时作主动脉斑块面积分析及HE染色等主动脉病理学检测,并取血管组织用HPTLC测量血管胆固醇的含量。
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Results The main structure of the left ventricle in canine was composed of the mitral complex、aortic valve、falsetendons and so on,among them the mitral complex was included in the mitral ring and valve,chordae tendinease and papillary muscles;the circle of mitral ring was 49.16±10.00mm,the average heights of inside and outside valves were 14.28±3.14mm,9.27±1.99mm,respectively,the breadth were 25.74±5.21mm、21.50±6.33mm;the average number of chordae tendineass was 16.72±2.76;the average number of left ventricular false tendon was 4.69±3.38:the circle of aortic valve ring was 34.55±8.00mm,the average heights of left、right and back aortic valves were 6.85±1.76mm、7.19±1.75mm and 7.52±1.79mm.
结果:犬心左心室主要结构有二尖瓣复合体、主动脉瓣、假腱索等,其中二尖瓣复合体由瓣环、瓣膜、腱索、乳头肌构成;二尖瓣瓣环周径为49.16±10.00mm,内侧瓣、外侧瓣的高度分别为14.28±3.24mm、9.27±1.99mm,其宽度分别为25.74±5.21mm、21.50±6.33mm;腱索总数平均为16.72±2.76条;假腱索总数平均为4.69±3.38条;主动脉瓣瓣环周径为34.55±8.00mm,左瓣高度、右瓣高度和后瓣高度分别为6.85±1.76mm、7.19±1.75mm和7.52±1.79mm。
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Results The body weight, visceral fat and body weight ration, as well as the levels of blood glucose, insulin, triglyceride, total cholesterol, LDL, and total FFA, were significantly lower in high fat-fed group than in control group (P.01). The aortic tunica intima was intact, the number of foam cells was decreased, and inflammatory cells infiltrated into the med-intima smooth muscle of the n-3 PUFA group. However, with no such changes were observed in control and n-3 PUFAs groups.
结果 n-3脂肪酸组大鼠体重第1~11周均明显降低,空腹血糖、血清胰岛素、TG、TC、LDL-C、总FFA和内脏脂肪占体重百分比均明显低于HF组,HDL-C则明显高于HF组,与对照组无明显差别;n-3脂肪酸组大鼠主动脉主动脉内膜完整无内皮细胞脱落现象,内膜下泡沫细胞及炎细胞浸润减少,内膜的NF-κB呈弱阳性表达,IκBα呈阳性表达,ICAM-1和MCP-1均呈阴性或弱阳性表达。
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The aortic tunica intima was intact, the number of foam cells was decreased, and inflammatory cells infiltrated into the med-intima smooth muscle of the n-3 PUFA group. However, with no such changes were observed in control and n-3 PUFAs groups. Little or no expression of NF-κB, ICAM-1 and MCP-1, and a high expression level of IκBα were detected in aortic tunica intima of the n-3 PUFA and control groups, while high expression levels of NF-κB, ICAM-1 and MCP-1, and little expression of IκBα were detected in aortic tunica intima of the high fat-fed group.
结果 n-3脂肪酸组大鼠体重第1~11周均明显降低,空腹血糖、血清胰岛素、TG、TC、LDL-C、总FFA和内脏脂肪占体重百分比均明显低于HF组,HDL-C则明显高于HF组,与对照组无明显差别;n-3脂肪酸组大鼠主动脉主动脉内膜完整无内皮细胞脱落现象,内膜下泡沫细胞及炎细胞浸润减少,内膜的NF-κB呈弱阳性表达,IκBα呈阳性表达,ICAM-1和MCP-1均呈阴性或弱阳性表达。
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Results From the fourth week, in diabetic rats, the contents of AGEs in plasma and the abdominal aorta increased, the relative area of α-SM-actin in the abdominal aorta decreased, the density of nucleus of VSMC increased, and mitochondria and rough endoplasmic reticules in VSMC increased.
结果 从4周起,DM大鼠血浆和腹主动脉的AGEs显著增多;腹主动脉α-SM-肌动蛋白的相对面积减小,VSMC的细胞核密度增大,线粒体和粗面内质网增多。
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Congenital aortic stenosis occurs due to improper development of the aortic valve in the first 8 weeks of fetal growth.
主动脉狭窄的发生是由于在胎儿生长发育的前8周里主动脉瓣的不正常的发育。
- 推荐网络例句
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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.
随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。
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But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.
不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。
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Closes the eye, the deep breathing, all no longer are the dreams as if......
关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。