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Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.

采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型,分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本,行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况;行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润;行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达;分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。

The blood samples were collected at pre-operation, aortic clamp on, aortic clamp off, CPB wean off, MUF cessation, post-operative 2 hours and post-operative 20 hours for measurement of the concentration of glucose, protein and fatty acid.

选取40例15 kg以下先天性心脏病患儿,分别在手术开始、主动脉阻断时、主动脉开放、CPB结束、改良超滤后、术后2 h和20 h测定血糖,游离脂肪酸,血清总蛋白。

METHODSForty children, less than 15 kg, with congenital heart defects were chosen in the study. The blood samples were collected at pre-operation, aortic clamp on, aortic clamp off, CPB wean off, MUF cessation, post-operative 2 hours and post-operative 20 hours for measurement of the concentration of glucose, protein and fatty acid.

选取40例15 kg以下先天性心脏病患儿,分别在手术开始、主动脉阻断时、主动脉开放、CPB结束、改良超滤后、术后2 h和20 h测定血糖,游离脂肪酸,血清总蛋白。

Irregular reddish tan vegetations overlie valve cusps that are being destroyed.

不规则的红棕色疣状赘生物覆盖在主动脉的表面,而引起主动脉瓣膜被损伤。

Methods Twenty-six NZW rabbits were randomly divided into three groups: a normal control group (fed on normal commercial rabbit diet, n =6), a hyperlipidemic group fed on high-fat ma total cholesterol (TC concentration at the beginning and the 12-week end point of the experiment determined,and all the experimental rabbits were sacrificed, and endothelial function test were performed on thoracic aortic rings prepared from the isolated aorta using the vascular endothelium-dependent relaxation parameter in response to acetylcholine The aortic atherosclerotic lesion expression of LOX-1 mRNA and protein were examined by RTPCR and Western blotting respectively.

方法新西兰大白兔随机分为正常对照组(普通饮食,6只),高脂饮食组(高脂饮食,10只)及普罗布考组(高脂饮食+普罗布考200mg/kg·d,10只)。实验开始前及第12周分别耳缘静脉采血测定血清总胆固醇。第12周处死动物,取胸主动脉,制备离体胸主动脉环对乙酰胆碱的反应以检查内皮功能,RT-PCR与免疫印迹检测凝集素样氧化低密度脂蛋白受体-1(LOX-1)基因与蛋白质表达水平。

After being registered accurately with Photoshop 7.0 software, the male urological and reproductive organs such as kidney, adrenal, ureter, bladder, male urethra, prostate, spermaduct, seminal vesicle and the adjacent organs such as abdominal aorta, inferior vena cava, thoracic duct, the lymph nodes beside abdominal aorta, the nephritic artery and vein, the intrailiac artery and vein, pelvis, the prostatic plexus and internal obturator muscle can be segmented and surface rendering three-dimensional reconstructed with 3D-DOCTOR software.

断面图像数据集经Photoshop 7.0软件精确对位、配准后,应用3D-DOCTOR 4.0软件,分别对男性泌尿生殖系统的肾脏、肾上腺、输尿管、膀胱、男性尿道、前列腺、输精管、精囊腺和泌尿生殖系统的毗邻器官腹主动脉、下腔静脉、胸导管、主动脉旁淋巴结、肾动静脉、髂内外动脉、骨盆、前列腺周围静脉丛、闭孔内肌等进行数据分割和三维面绘制重建。

With 3D-doctor software, kidney, adrenal, ureter, abdominal aorta, inferior vena cava, the nephritic artery and vein, the lymph nodes beside abdominal aorta, thoracic duct, the intrailiac artery and vein, pelvis, spermaduct, prostate, the prostatic plexus, bladder, male urethra and internal obturator muscle were segmented and three-dimensional reconstructed in P4 computer. 3. With photoshop7.0 software, the major organs of the male urological and reproductive system such as ureter, bladder, urethra, prostate were segmented and the grey- color mode images through segmentation were imported to AMIRA software.

应用连续图像分割软件3D-DOCTOR,分别对肾脏、肾上腺、输尿管、腹主动脉、下腔静脉、肾动静脉、腹腔淋巴结、胸导管、主动脉旁淋巴结、髂内外动静脉、骨盆、股骨、输精管、附睾、前列腺、前列腺周围静脉丛、膀胱、男性尿道、直肠、闭孔内肌、肛提肌、尾骨肌等结构进行结构数据的分割与提取,并在PentiumⅣ计算机上完成已分割结构的三维重建。

Operation methods: A needle (diameter 0.7mm) was inserted and ligated in the abdominal aorta, and then the stylet was drawn out.

造模方法:在大鼠肾动脉上方约1cm的腹主动脉处,将直径0.7mm的注射针头与腹主动脉一起结扎,然后抽出针头。

Methods: Sprague Dawley rats have been used as recipients and Wistar rats as donors. Donor aorta was anastomosed to the recipient's suprarenal abdominal aorta; donor pulmonary artery was anastomosed to the left renal vein of the recipient using continuous suture technique.

以Wistar大鼠为供体,SD大鼠为受体,将供心的主动脉和肺动脉分别与受体肾血管上方的主动脉和左肾静脉用连续缝合技术行端侧吻合。

There were 3231 patients with isolated mitral valve replacement , 917 patients with isolated aortic valve replacement , and 48 patients with tricuspid valve replacement .

其中二尖瓣置换术3231例,主动脉瓣置换917例,三尖瓣置换48例,二尖瓣和主动脉瓣置换1296例,其他14例。

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