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In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较,同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系,及其免疫原性的变化规律,通过DNA重组技术,设计了一对引物LHMP7/LHMP8,该对引物选取位于2885~2900及4618~4604的两段序列,跨幅为1734bp,并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点,分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增,并将PCR产物克隆到pMD18-T载体上,分别得到二个重组子:pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

Oh my lord, to be near you, to be free.

噢,我的主,为了你,为了自由。

To be near you, to be free.

为了和你在一起,为了自由

I suspect that the immense power of the computer is being harnessed to this 'paper economy', not to do the same transactions more economically but to balloon the quantity and variety of financial exchanges…I fear that, as Keynes saw even in his day, the advantages of the liquidity and negotiability of financial instruments come at the cost of facilitating nth-degree speculation which is short-sighted and inefficient.

我认为计算机的巨大力量被"虚拟经济"所挟持,不是为了更经济地执行交易而是为了扩张金融交易的数量和种类……我担心,正如凯恩斯当年就发现的,金融工具的流动性、流通性所带来的好处是以助长短视且无效的N倍投机活动为代价的。

This is not for the hobby of myself only, also be to judge the place of Ebay, want to know, the company that why fills in to this California emperor, they at the outset core negotiant is those collect lover actually.

这不单单是为了我自己的爱好,也是为了判断Ebay的处境,要知道,对于这家加州圣何塞的公司而言,他们当初的核心交易者其实就是那些收藏爱好者。

Is neI know you didn't do this just to win an election and I know you didn't do it for me.

我知道你们的所做所为并不只是为了赢得大选,我也知道你们做这一切并不是为了我。

Their costumes are content to express sympathy with the music content is to embody the punk theme, how can one to the "non-mainstream" people who do not know on the neither fish nor fowl

他们的服饰都是有内容的,是为了表达与其音乐内容的共鸣,是为了体现朋克主题,怎么一到了"非主流"身上就不伦不类的让人不知

Romeo, there dead, was husband to that Juliet; And she, there dead, that Romeo's faithful wife: I married them; And their stol'n marriage-day was Tybalt's dooms-day, whose untimely death Banish'd the new-made bridegroom from the city, for whom, and not for Tybalt, Juliet pined.

死了的罗密欧是死了的朱丽叶的丈夫,她是罗密欧的忠实的妻子,他们的婚礼是由我主持的,就在他们秘密结婚的那天,迪伯尔特的死于非命,这位才做新郎的人也从这城里被放逐去;朱丽叶是为了他,不是为了提伯尔特,才那样伤心憔悴。

The purpose of Niebuhr's violent and fatal attack on Liberalism is not to end it but is to reconstruct it, to make it get a new life.

尼布尔对老自由主义的致命批评,不是为了推翻自由主义,而是为了改造自由主义,使其获得新的生命。

The emphasis on agricultural development now is not only for its instrumental value in sustaining expansion elsewhere in the nonagricultural sectors, but for its own absorption of labor and its own increase of real income among the rural poverty group of farmers.

农业发展的重要性不仅是维持非农业部门稳定发展的基础,而且具有为了增强自身吸收农业劳动力的能力以及为了提高农村贫穷农民的可支配收入的作用。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。