为...开放

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

In details, the development path of our automobile industry under the background of the industry globalization includes: grow active and fully competitive local market environment; it is not only the enter of several multinational companies fixing attention on global strategy but also the independent development of local excellent business enterprise, elaborate the competitive ability of native automobile industry wholly in the end; melt into the automobile industry system in global division openly and gradually, which will carry out the long-term target of raising the international competitive ability and the net export; carry out the development strategy focusing on the medium-low-class household-use car industry in the middle period; reorganize the industry in part facing to the global market; transit to the high of the industry chain increasing in value dividedly.

具体来说，产业全球化背景下我国汽车产业发展的路径选择包括：培育积极而充分竞争的国内市场环境；着眼于全球战略的多个跨国公司进入，与国内优秀企业自主发展并举，最终整体上提高我国本土汽车产业的竞争力；开放中逐步融入汽车产业全球分工体系，将提高国际竞争力、实现净出口作为中长期目标；以中低级别家用车为重点的中期发展战略；面向全球市场的零部件工业重组；分阶段向产业增值链高端过渡。

1976 " The Great Cultural Revolution " convulsion period, be disturbed badly and national economy progress is destroyed, manufacturing efficiency and economic benefits drop, but because save indefatigable effort of people completely, there still is certain development eclectically in hard music. 1978 11 of the party 3 in since plenary meeting, bring order out of chaos, hold high Deng Xiaoping academic banner, hold to the main line of the party, insist to be a center with economic construction, hold to 4 basic principles, emancipatory thought, renew an idea, deepen reform, expand open, accelerate economic system and the change that economy grows way, initiated since founding a state, the richest lease of life is mixed the new period of vigor.

在旧社会遗留下来的军疮百孔、支离破碎的基础上，初步建立了门类比较齐全的国民经济发展体系，各项建设事业都取得了令了瞩目的成就。1966—1976年的&文化大革命&动乱时期，国民经济发展受到严重干扰和破坏，生产效率和经济效益下降，但由于全省人民的不懈努力，在艰难曲折中仍有一定发展。1978年党的十一届三中全会以来，拨乱反正，高举邓小平理论旗帜，坚持党的基本路线，坚持以经济建设为中心，坚持四项基本原则，解放思想，更新观念，深化改革，扩大开放，加快经济体制和经济增长方式的转变，开创了建国以来最富有生机和活力的新时期。

Yamaxun is at opening a name to developer last year " elastometer calculates machine cloud " service, IBM also is rolled out " La Yun " plan.

亚马逊于去年向开发者开放了名为&弹性计算机云&的服务，IBM 也推出&蓝云&计划。

Fluctuating number of windows have not lived in the number of hotels will feel wronged feelings of separation is used to browse the collection or used them so How long have toiled every day unforgettable calamity wet flies long sad eyes can see is the legacy of love for vagrants and a few have double beds several rings resolute belief before the exchange warmth to a person's chest for the last mistakes committed by another reflection Everyone dreams are out on tenterhooks refused to do so enjoyed the lamb love Guilt is not track on the Moon enclasp change the silhouette disappeared in the darkness of false circulation was fine in the sun all obstacles want to be bound to God to forgive non-stop love need more courageous no answers as the candlelight dinner dinners -- love is not a warm embrace for petals spread on the bed sheets it grow too crowded, open to the emotional needs of other people came close to the soil return to the vicious cycle of short-term expectations disappointed expectations always dissatisfied with the Federation of romantic long lost youth for a better husband to be your best not to be disappointed exciting ordinary

浮动视窗数目没有住在酒店数量将抱屈感情是用来分离浏览收集或使用它们每天辛辛苦苦做多久难忘浩劫湿苍蝇长伤心眼睛所看到的却是爱流浪的遗产数有几个戒指坚定信仰双人床前外汇温暖一个人的胸口上犯了错误的又一生动体现，是人人梦想如坐针毡拒绝这样做羔羊爱有罪不享受在月球轨道握紧改变身影消失在黑暗虚假流通晴朗太阳一切障碍不受约束地上帝原谅不停荒更需要勇气爱情是没有答案的烛光晚餐晚餐--不是爱情拥抱为花瓣的床单它蔓延成长太拥挤开放的情绪需要别人差点土壤回到恶性循环短期期望期望总是失望不满联合会青年对美好爱情早已失去丈夫当您最好不要失望兴奋普通

Analysis of the different region by using bioinformatics tools, National center for biotechnology information Basic Local Alignment Search Tool, we found that there is an open reading frame being predicted as an endolysin gene.

以National center for biotechnology information Basic Local Alignment Search Tool生物资讯工具分析此段序列，发现其中有一开放读架预测为溶菌酶基因。

It is an open space with the pavement equiped, ready to receive the adequate facilities to become a single person house.

这是一个有人行设备的开放的空间，即将安装适当的洗浴和厨卫设施使其成为一个单身住所。

The case study found: the boy's play therapy went through 3 phrases, including "knowing the therapeutic conditions well","establishing of therapeutic relationship","developing play therapy evolutionally"; the clues from which to judge relationship included "verbal and nonverbal interaction between therapist and the boy","the boy's dealing with his parents ","his 'first' behavior", the aim of establishing was to form "mother-child unity"; the relationship between therapist and the boy's parents passed through "admitting the facts","opening their feelings" and "detailed guidance"; the development of the boy's play consisted in the larger degree of digging and moving sand(showing that the therapy went deep into the boy's mind and his sense of strength was improving), the increasing of imaginative plays(showing that the boy began to pay attention to others and knew how to play), from playing by himself to cooperating with therapist(showing that he felt the happiness in cooperating and interacting with others and was gaining self-confidence which was favorable to be transferred to his lives and be used to communicating with others), sand and water were very important therapeutic factors for the boy(sand could mobilize his sense of feeling and touching , awakening his energy and regulating it. water could provide relaxed feeling, sense of being contained and satisfied and enlighten his imagination).

研究发现，个案的游戏治疗经历了三个阶段，即&熟悉治疗环境阶段&、&治疗关系建立阶段&和&游戏治疗深入发展阶段&；考察治疗关系建立的指标包括&个案与治疗者之间的言语和非言语互动&、&个案与父母的关系处理&和&个案的'第一次'行为&，建立的目标是&母子一体性&的形成；治疗者与个案父母关系的建立经过了&接纳事实&、&开放情感&和&具体指导&三个阶段；个案在治疗中游戏的发展表现为：动沙的力度增大，说明治疗深入了个案内心，其自我能量感在增强，游戏中的想象性成分不断增多，说明其对周围事物和他人关注了，开始&会&游戏，从单独游戏逐渐向简单合作游戏发展，说明其体验到了合作和互动的快乐，并从中渐渐获得自信，有助于个案将这种体验和自信迁移到日常生活中，尝试与他人互动；沙和水是个案游戏治疗中的重要治疗因素，沙可以调动自闭症幼儿的感触觉，唤醒其潜在能量，调整能量分配，水给自闭症儿童一种轻松感、包容感和满足感，并引发其想象。

The case study found: the boy"s play therapy went through 3 phrases, including "knowing the therapeutic conditions well","establishing of therapeutic relationship","developing play therapy evolutionally"; the clues from which to judge relationship included "verbal and nonverbal interaction between therapist and the boy","the boy"s dealing with his parents ","his "first" behavior", the aim of establishing was to form "mother-child unity"; the relationship between therapist and the boy"s parents passed through "admitting the facts","opening their feelings" and "detailed guidance"; the development of the boy"s play consisted in the larger degree of digging and moving sand(showing that the therapy went deep into the boys mind and his sense of strength was improving), the increasing of imaginative plays(showing that the boy began to pay attention to others and knew how to play), from playing by himself to cooperating with therapist(showing that he felt the happiness in cooperating and interacting with others and was gaining self-confidence which was favorable to be transferred to his lives and be used to communicating with others), sand and water were very important therapeutic factors for the boy(sand could mobilize his sense of feeling and touching , awakening his energy and regulating it. water could provide relaxed feeling, sense of being contained and satisfied andenlighten his imagination).

研究发现，个案的游戏治疗经历了三个阶段，即&熟悉治疗环境阶段&、&治疗关系建立阶段&和&游戏治疗深入发展阶段&；考察治疗关系建立的指标包括&个案与治疗者之间的言语和非言语互动&、&个案与父母的关系处理&和&个案的'第一次'行为&，建立的目标是&母子一体性&的形成；治疗者与个案父母关系的建立经过了&接纳事实&、&开放情感&和&具体指导&三个阶段；个案在治疗中游戏的发展表现为：动沙的力度增大，说明治疗深入了个案内心，其自我能量感在增强，游戏中的想象性成分不断增多，说明其对周围事物和他人关注了，开始&会&游戏，从单独游戏逐渐向简单合作游戏发展，说明其体验到了合作和互动的快乐，并从中渐渐获得自信，有助于个案将这种体验和自信迁移到日常生活中，尝试与他人互动；沙和水是个案游戏治疗中的重要治疗因素，沙可以调动自闭症幼儿的感触觉，唤醒其潜在能量，调整能量分配，水给自闭症儿童一种轻松感、包容感和满足感，并引发其想象。

The site is located in the heart of Shaba Hotel zone in the east corner of the middle Haitang Bay in Hainan: it faces the South Sea and artificial island reef, possessing the excellent view of Wuzhizhou Island; to the east, it is near the fingerlike wetland, and the seafront landscape channel throughout the island provides good availability ; there is planned to be open space on the north and south sides, which offers unique confidentiality and exclusive enjoyment, and creates better sea view.

该项目基地位于海南海棠湾中部东隅沙坝酒店地带核心位置：东临南海及人造岛礁，拥有对蜈支洲岛优越的视线；西靠指状湿地，贯穿全岛的滨海景观道提供良好的可达性；南北两侧规划大尺度开放空间，为本项目提供了独特的私密与独享性，同时创造了更好的面海视野。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。