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The acquired gene was 603 bp in length, including 96 bp of 5' untranslated region, 222 bp of 3' untranslated region, and an open reading frame of 285 bp.

基因的5'非翻译区96 bp, 3'非翻译区222 bp,开放阅读框 285 bp,编码94个氨基酸,预计蛋白的分子量为9.9 kD,等电点为8.02。

The deduced amino-acid sequence of the TcGH had 210aa,including a putative signal peptide (22aa) located at its N-terminal. The homology of the TcGH amino-acid with relative species grass carp,black carp,bighead carp,carp,zebra fish,etc.

结果序列分析表明:唐鱼GH cDNA的5 非编码区为64 bp,3 非编码区为448 bp,开放阅读框633 bp,共编码210个氨基酸,包括22个氨基酸的信号肽和188个氨基酸的成熟肽。

In this paper, the cDNA sequence from Ampullaria crossean stomach named Dy1300 was obtained by RT-PCR firstly and cloned into vector pMD 18-T. Its ORF named SXYN contains 882bp nucleotide encoding a 294 amino-acid protein. The percentage of similarity of SXYN with the reported cellulase EGX gene sequence from Ampullaria crossean was 89.8%.

本研究首先通过RT-PCR的方法从福寿螺胃中扩增出新的纤维素酶cDNA序列―Dy1300,并与克隆载体pMD 18-T相连接,通过对其全序列的分析,确定其开放阅读框ORF长度为882bp,命名为SXYN(GenBank, AY941794),推定的氨基酸序列含294个氨基酸,与已报道的福寿螺纤维素酶C端基因的同源性达89.8%。

With the trend of utility, common customs and manifold, country religion is more and more autochthonous.

认为改革开放以来江浙乡村的&宗教热&具有与此前不同的三大特征:宗教信众的增加主要集中在一些经济极发达地区和一些经济极贫困地区,乡村宗教发展呈现出两极化的趋势;佛教、道教等本土化宗教继续衰落或复兴无力,而天主教和基督教这些非本土的宗教却在农村迅速拓展,呈现为&西教东热&的趋势;宗教信仰功利化、信仰群体多元化乡村教会世俗化,呈现为适应江浙乡村社会现实的本土化趋势。

CIG, continuing to persist in the tenet of "open, cooperative, mutual beneficial and double win" and the digital entertainment concepts of "green games and healthy life", will contribute to the healthy and harmonious development of the digital entertainment industry in China which takes the net games as the core.

CIG将继续坚持&开放合作,互利共赢&的宗旨,秉承&绿色游戏、健康生活&的数字娱乐理念,为推进中国以网络游戏为核心的数字娱乐产业健康协调发展做出贡献。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用,并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1),另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死,取出损伤节段脊髓用来组织病理学检查,同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过,到损伤后28天,我们观察发现,损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18,group 1∶9.89±1.29;p.05),光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

The cage effect of Gn potassium aqueous solution calculated by theα-cleavage photoreaction product distribution of substituted dibenzyl ketone was 14%, 18%, 22% and 42% for generations 1-4, respectively, which indicates that G4 takes a more close spherical shape with strong confine ability.

研究结果表明,羧基为末端基团的芳醚树枝形聚合物Gn (n = 1-4)钾盐水溶液笼效应随代数增加而增大,从G1到G4,笼效应分别为14%、18%、22%和42%,树枝形聚合物3代到4代从开放结构变为近乎球形的封闭结构的显著变化使G4表现出明显高于其他三代的笼效应。

Leaves alternate, distichous or spirally arranged, sessile or petiolate; leaf sheath prominent, open or closed; leaf blade simple, entire. Inflorescence usually of cincinni in panicles or solitary, sometimes shortened into heads, sometimes sessile with flowers fascicled, sometimes axillary and penetrating enveloping leaf sheath, rarely flowers solitary and terminal or axillary.

叶互生,二列或者螺旋状排列,无柄或者有柄;叶鞘显著,开放或者闭合;单叶,全缘;花序通常为蝎尾状圆锥花序或者单花,有时缩短常为头状花序,有时无梗,花簇生,有时腋生,贯穿叶鞘,很少花单生,顶生或腋生。

First, we discuss a monopolistic downstream firm's decision of outsourcing in the final good market. Then we extend the model to a duopolistic downstream market structure with two competing firms to examine whether the outsourcing decisions change in such a competitive environment.

模型推导以渐进式进行,首先讨论当最终财市场结构为独占下,最终财厂商的委外决策;其次开放市场竞争程度,使最终财市场结构由独占转为寡占,厂商家数由一扩增为二,且两厂商在最终财市场进行同质产品的数量竞争,则最终财厂商的委外决策是否改变。

So we adopted the isotope marking method in order to know the distribution of BBP in male and female mice. The result indicated 〓H-BBP presented the first-order kinetics and matched the open two-compartment model. Distributional half-life was about 20 minutes and eliminable half-life was about 6-7 hours. As α(the speed constant of distributional phase) was about ten times β(the speed constant of eliminable phase), this compound had a fast distribution and a low elimination.

为了揭示BBP致毒有明显性别差异的原因,采用同位素标记方法示踪BBP在雄、雌动物体内的归趋,结果表明:BBP在动物体内呈一级动力学线性过程,符合二室开放模型,分布半衰期为20min左右,消除半衰期为6-7hr左右,分布相的速度常数α是消除相的速度常数β的10倍左右,说明该化合物在体内的分布较快、消除慢的特征。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

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