中骨

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/LM-CSF和0，1，10，100μg/LTNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/LM-CSF和30μg/LsRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/LTNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

The results from the cluster analysis and the principle component analysis based on the mean measure of divergence of eight groups of Mongoloids show that the Guangrao skeletons belong to the East Asian Mongoloid group and we also find that mylohyoid bridge, double h...

同时本文结果提示，在明确蒙古人种地区类型的主成分分析研究中，贡献率较大的三个小变异特征为下颌舌骨肌线桥、舌下神经管二分、眶上孔，这将为以后人们用小变异方法从事这方面的研究中如何选择观察项目方面提供了基础

This review focused on the biological significance of matrix metalloproteinases in its regulation when mesenchymal stem cells differentiate into myoblastic, osteoblastic, chondroblastic, neural, and apidoblastic lineages.

文章详细阐述了基质金属蛋白酶在间充质干细胞分化为成肌细胞、成骨细胞、破骨细胞、神经细胞、脂肪细胞过程中所起的重要生物学作用，并对基质金属蛋白酶在间充质干细胞分化中的调控作用进行分析。

The effectiveness was evaluated by the analysis of the result of ophthalmological examination and the computed tomographic scanning.

非CAD/CAM治疗组包括自体骨移植、Medpor移植和术中成型钛网移植，术后通过眼科检查和CT分析评价CAD/CAM在眶壁重建中的应用价值。

Results The optimum conditions for the extraction of Peucedanum praeruptorum Dunn was as follow: 80 % alcohol was used as solvent, and 8 times the amount of alcohol was added, the mixture was kept refluxing for 1.0 h for the first time; then 6 times the amount of alcohol was added and the mixture was kept refluxing for 0.5 h for the second time; Four times the amount of alcohol for 0.5 h for the last time.

结果 白花前胡的最佳提取工艺为用质量分数80 %的乙醇提取3次，提取时间分别为1.0、0.5、0.5 h，3次所用溶媒量分别为药材质量的8、6、4倍；按照筛选出的最佳工艺条件进行的3次重复性实验，测得浸膏中8-甲氧基补骨脂素含量质量分数为0.094 %，折合1 g生药中含量为0.171 2 mg，与预测值基本一致。

However, the incidence of carrying mtDNA4977 deletions in heart and liver were no significant difference between presbycusis patients and the control group.

老年聋患者颞骨切片和蜗核组织中mtDNA4977缺失的发生率明显高于老年听力正常组，其差异具有显著性意义（χ2检验，P＜0.05）；而与听觉无关的心肌和肝脏组织中mtDNA4977缺失发生率在两组之间差异无显著性。

It is know that lead can distribute in the parenchyma at first,such as blood,and then can be stored in bone proximally 95% at last by redistribution.

现己经证明，铅在人体的分布，先在血液等软组织中进行，然后经再分布过程，最终铅负荷的95%储存在骨中。

Methods A retrospective analysis of refixation of fractured skull bone fragments was made. From January 2001 to November 2001, we operated on 52 patients with fractured bone fragments.

2 0 0 1年 1～ 11月行开颅手术的头部外伤患者中有 5 2例骨瓣碎裂呈多块，其中 2 4例应用颅骨钛铆钉回复固定颅骨骨折片，分析其术中操作、术后临床观察及CT检查等情况。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。