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Comparison the in vivo activity of 90%EtOH fraction and compound 21 with estradiol, indicated the 90%EtOH fraction behaved the stronger activity on total mineral content, total density, trabecular mineral content, trabecular density and the anti-twist strength of the proximal tibia in OVX rat than estradiol, but compound 21 displayed the weaker activity on total mineral content, total mineral density, trabecular mineral density, cortical mineral content, cortical mineral density, cortical thickness and the anti-twist strength of the proximal tibia than estradiol.

通过90%乙醇部位及化合物21都与阳性对照药雌二醇的纵向比较后,在大鼠OVX模型中,90%乙醇部位高剂量组在提高全骨的骨矿含量和骨密度、松质骨的骨矿含量和骨密度及骨的抗扭力方面,作用强于阳性对照组雌激素类化合物,化合物21在小鼠OVX模型中,抑制了全骨的骨矿含量、骨密度和全骨断面面积的降低,松质骨骨密度的降低,皮质骨的外膜周长的增长,皮质骨骨矿含量量、皮质骨厚度和皮质骨密度的下降及抗扭力的减弱。

Result:As compared with the model group, the effect of small dose group and middle dose group of osteopractic total flavone on TBV% of shankbone increased significantly; the effect of small dose group and middle dose group of osteopractic total flavone on TRS% of shankbone reduced significantly and TFS%, AFS%, MAR, BFR of shankbone reduced obviously; the effect of middle dose group of osteopractic total flavone on OSW and mAR reduced obviously, the effect of small dose group of osteopractic total flavone on them had the tendency of reducing, but there was no statistical significance.

结果 :对胫骨骨小梁体积百分比的影响,与模型组比较,骨碎补总黄酮小剂量组和中剂量组的TBV %明显增高;对胫骨骨小梁吸收表面百分比的影响,与模型组比较,骨碎补总黄酮小剂量组、中剂量组的TRS %显著降低;对胫骨骨小梁形成表面百分比,活性生成表面百分比,骨小梁矿化率和骨小梁骨生成率的影响,骨碎补总黄酮小剂量组、中剂量组的TFS %,AFS %,MAR ,BFR较模型组皆明显降低;对胫骨类骨质平均宽度和骨皮质矿化率的影响,骨碎补总黄酮中剂量组的OSW和mAR与模型组比较,明显降低;骨碎补总黄酮小剂量组与之相比,有下降的趋势,但无统计学意义。

Then the cultivated chondrocytes were embedded in fibrin glue fused on spongy bone, covered with priosteal flap; the complex was used to repair the femoral trochlea osteochondral defect which size is 3mm × 4mm × 4mm made in rabbit knee joint.

在A组的每只兔子的一侧膝关节股骨滑车部人为造成3mm×4mm的骨软骨缺损,骨刀切除软骨下骨到髓腔渗血为止(厚约4mm),压迫后FG止血;取髂骨骨块,并尽可能保留松质骨,取下的骨块用PBS反复清洗,以除去血细胞,将松质骨填充在骨缺损处,松质骨面朝向关节腔,高度与周边软骨下骨齐平,把骨膜片生发层朝向关节腔,用无创伤缝合线缝合在周边的软骨或滑膜组织上;向EP管中加入1/2悬液体积的FG主体胶溶液并混匀,再与主体胶等体积的催化剂溶液一同注射入骨膜与骨块密闭的腔隙中;同理处理另一侧膝关节。B组处理与A组相比只是不加入软骨细胞;C组造成骨软骨缺损,FG覆盖创面后单纯用骨膜修复缺损。

In order to isolate the active compounds in ethyl acetate fraction of Psoralea corylifolia L., activity-guided isolation was performed along with chromatographic techniques. Four active compounds were isolated from ethyl acetate fraction and identified by their NMR spectral data and physical-chemical properties. They are corylin and bavachin, which showed stimulating effect on osteoblastic proliferation and differentiation, isopsoralen and psoralen exhibited activity of promoting cell differentiation in some degree.

为了追踪分离补骨脂乙酸乙酯萃取物中的活性成分,本实验采用多种色谱分离技术,以活性测试为导向,结合波谱技术及理化数据,从补骨脂乙酸乙酯萃取物中分离并鉴定了4个活性化合物的化学结构,它们是具有促进细胞增殖和分化作用的补骨脂异黄酮和补骨脂二氢黄酮,对细胞分化有一定促进作用的异补骨脂素和补骨脂素。

The advantages of dual grafts for bridging defects are as follows:(1) mechanical fixation is better than fixation by a single onlay bone graft;(2) the two grafts add strength and stability;(3) the grafts form a trough into which cancellous bone may be

双侧植骨桥接骨缺损的优点包括:①机械固定作用比单侧贴附植骨更好;②双侧植骨可增加强度和稳定性;③双侧的植骨可形成一骨槽,可在其中压紧松质骨;④与单侧植骨不同,双侧植骨在愈合过程中可防止挛缩的纤维组织危害移植的松质骨。

The adantages of dual grafts for bridging defects are as follows:(1) mechanical fixation is better than fixation by a single onlay bone graft;(2) the two grafts add strength and stability;(3) the grafts form a trough into which cancellous bone may be packed; and (4) during healing the dual grafts, unlike a single graft, preent contracting fibrous tissue from compromising transplanted cancellous bone.

双侧植骨桥接骨缺损的优点包括:①机械固定作用比单侧贴附植骨更好;②双侧植骨可增加强度和稳定性;③双侧的植骨可形成一骨槽,可在其中压紧松质骨;④与单侧植骨不同,双侧植骨在愈合过程中可防止挛缩的纤维组织危害移植的松质骨。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果:骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后,可见下领升支外侧的骨皮质上有新骨形成,新骨呈山峰状凸起,术后第28天的新生骨较低平,多孔隙,随着固定时间的延长,新骨逐渐成熟;下领升支前后向切线位X线投照显示新骨呈山峰样隆起;经测量,术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm;脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长,以及胶原纤维和骨小梁排列方向上的变化,术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示,与对照组相比较,实验侧骨膜增生明显,细胞间排列紧密,单位面积内骨膜细胞数增多,细胞核饱满;骨膜PCNA 免疫组化染色显示,与对照侧相比较,实验侧骨膜在牵张后出现了明显的增生迹象,PCNA阳性细胞分布紧密,单位面积内阳性细胞数较对照组多,靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

The technique of bone segment frifting is a simple and effective method for bone defect.Traumatic face of soft tissue can decrease and close,and the bone infection can be controlled gradually during the bone segment frift.Finally,the infection can be healed with the bone union.

结论骨段滑移技术是治疗下肢大段骨缺损简单而有效的方法,软组织感染创面可在骨段滑移中逐渐缩小并闭合,骨感染可在骨段滑移中逐渐被控制并随骨连接而愈合。

Objective: To explore the effect of ovariectomy caused Kidney Deficiency on the metabolism of bone in mice with collagen induced arthritis.

目的:探讨卵巢切除所致肾虚影响免疫性关节炎中骨代谢的机制。

To explore the effect of ovariectomy caused Kidney Deficiency on the metabolism of bone in mice with collagen induced arthritis.

目的:探讨卵巢切除所致肾虚影响免疫性关节炎中骨代谢的机制。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?