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Rat ventral mesencephalic precursors can be induced to differentiate into plenty of tyrosine hydroxylase-positive DNs, mainly originating in A10 regions.

大鼠腹侧中脑多巴胺能前体细胞可在体外大量诱导分化成TH阳性的主要源自A10区的多巴胺能神经元。

This article explores the culture methods for the differentiation of mesencephalic neural stem cells from embryonic mice into dopaminergic neurons by interleukin-1β in vitro.

以白细胞介素1β为诱导剂,观察鼠胚胎中脑神经干细胞向多巴胺能神经元的分化。

MPP is what really works.Many in vivo studies have proved MPTP can cause PD.We are interested in investigating the influence of MPP+ on mesencephalic Dopamine neurons in vitro and to make sure whether it can be used as an PD model in vitro.

本实验研究拟通过MPP~+对体外培养的中脑多巴胺能神经元的影响的观察探讨MPP~+致PD发病的可能机制以及用制备MPP~+体外的PD模型的可能及其价值。

Rat mesencephalic ventral brain tissue was isolated for culture of neural stem cells.

①分别于大鼠孕10,12,14,16,18天摸球法随机选取6只,麻醉后处死孕鼠,无菌条件下剖腹取胚胎,分离中脑腹侧脑组织进行神经干细胞培养。

Extracellulary recording method was used to examine the effect of thyrotropin-releasimg hormone on 71 unit discharges of pain excited neurons in mesencephalic reticular formation of 58 rats.

用细胞外记录单位放电和脑室微量注射的方法,在58只大鼠的中脑网状结构内,观察了71个痛兴奋神经元对促甲状腺素释放激素的放电反应。

Mesencephalic neural stem cell biological characteristics, isolation, proliferation and differentiation are subject to further study. In addition, biological safety problems using mesencephalic neural stem cells in the human body gradually attracted the attention of the people.

同时中脑神经干细胞应用于人体治疗的生物安全性问题也逐渐引起人们的重视,如在体外多次传代的神经干细胞是否会发生转化,是否会带来一定的副作用,甚至致瘤性等均在进一步探索中。

Results: the number of th positive cells and the viability of mesencephalic cells significantly decreased while the number of inos cells increased in the development of apoptosis induced by mpp+.

结果:在mpp+诱导的中脑原代细胞凋亡中,mpp+组th阳性细胞数及细胞存活率均显著低于各egb761组及阳性对照组(p.01)。

AIM: Biological function and in vivo survival of differentiated dopaminergic neurons, and comparison to the transplantation of embryonic mesencephalic DNs for treating Parkinson's disease rats are seldom studied.

目的:目前对于体外诱导分化的多巴胺能神经元的生物学功能、体内存活状况,以及与胚胎中脑多巴胺能神经元移植治疗帕金森病大鼠的疗效比较方面尚缺乏深入的研究。

In order to explore the function of glia in the progress the experiment was performed both in presence of glia and in the absence of glia The mouse mesencephalic cells were exposed with MPP+ with different concentration. Iloprost was used to investigate whether it can prevent dopamine neuron from the neurotoxic of MPP~..

为了观察胶质细胞在其中的作用,将细胞分为未抑制胶质细胞和胶质细胞抑制组;将不同浓度的MPP~+加入体外培养的中脑多巴胺能神经元培养液中,并加入Iloprost来研究其对MPP~+所导致的神经毒作用是否有保护作用。

The primary cultured fetal mesencephalic neural stem cells are passaged every 7days.

将人胚中脑来源的神经干细胞体外长期培养,每隔5-7天传代一次。

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