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Strong staining of ganglion cells in the Auerbach's and the Meissner's plexus were observed on the normal specimens. There was very little or no immunoreactive fibre in the lamina propria and muscularis mucosae of the aganglionic bowel. However there was strong immunoreactivity in the perineurium of the hypertrophic nerve trunks in the submucosal and intramural layers of aganglionic segments.

结果: 正常肠段的粘膜固有层和粘膜肌层中有大量的P75NGFR阳性纤维,粘膜下和肌间神经丛中神经节细胞表现为P75NGFR强阳性,痉挛段肠管粘膜固有层和粘膜肌层中P75NGFR阳性纤维明显减少或缺如,粘膜下和肌间粗大神经纤维的神经束膜表现为P75NGFR强阳性,象一个环包绕在周围。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因,根据鸡VIP基因(GenBank登录号X80906),设计了一对简并引物,从北京鸭腺胃、十二指肠和空肠提取总RNA,通过反转录-聚合酶链反应扩增,将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上,导入大肠杆菌JM109,阳性克隆经双酶切鉴定后测序,将测序结果与鸡和鹅(GenBank登录号为DQ023161)的VIP基因进行同源性比较。

Because of the significance of zebrafish Dapper2 in mesoderm induction, the mouse dapper2 was cloned following its homolog. The study of its spatial and temporal expression pattern showed that it expressed specifically in otic vesicle, somite,gut and roof plate of mouse embryos.

鉴于Dapper2对于胚胎中胚层发育的重要性,本文首先克隆出小鼠Dapper2基因,并研究其时空表达谱,发现它在体节形成期后在体节、耳泡、神经顶板、肠等组织中特异性表达。

A kind of virus particles were observed in cytoplasm of sampled ill sea cucumber tissues which were sampled from sick sea cucumbers with ulcerous skin and swollen mouth from their farming ponds at the beginning of 2005. The particles were covered with a bilayer envelops, shaped in globular with its size 100-200 nm in diameter. The same virus was isolated from cultured sea cucumbers with disease from different time and sites, and then infected the healthy sea cucumber. sea cucumber appeared the phenomenon such as ulcerous skin and swollen mouth and the same virus was isolated from infected sea cucumbers.

本章从患病刺参中分离出了2株对刺参具有感染能力的细菌,经鉴定1株为弧菌科弧菌属Vibrio sp和1株为弧菌科施万氏菌属Shewanella sp;在患病刺参的触手、肠和呼吸树等组织的细胞质中,检出了球形、具双层囊膜、直径大约为100-200nm的病毒粒子利用分离的病毒进行人工感染实验,刺参可以产生与自然发病刺参相同的症状,显示了分离的病毒对刺参具有一定的感染性。

Methods Strains of E. coli, Pseudomonas aeruginosa and Enterococcus faecalis were added in the model biles containing diffenent concentration of unconjugated bilirubin. Observed the change of cholesterol nucleation time.

大肠杆菌、铜绿假单胞菌和粪肠球菌分别加入含有不同浓度游离胆红素的模拟胆汁中,观察胆汁中胆固醇晶体形成时间的变化。

A glance at that dark, unstitched wound and a deep fissure in my brain opens up: all the images and memories that had been laboriously or absentmindedly assorted, labeled, documented, filed, sealed and stamped break forth pellmell like ants pouring out of a crack in the sidewalk; the world ceases to revolve, time stops, the very nexus of my dreams is broken and dissolved and my guts spill out in a grand schizophrenic rush, an evacuation that leaves me face to face with the Absolute.

所有以前费力地或心不在焉地分门别类、贴标签、引证、归档、密封并且打上印戳的印象和记忆乱纷纷一涌而出,就像一群蚂蚁从人行道上的一个蚁穴中涌出。这时地球停转了,时间停滞了,我的梦之间的相互联系也断了、消逝了,在精神分裂症大发作中我的肚肠流出来,这一次大扫除后我就与上帝面对面站在一起了。

In this paper, the cloning, expression and phylogenetic analysis of AmphiSDHD and AmphiS27 from the gut cDNA library of amphioxus Branchiostoma becheri tisingtauense are carried out.AmphiSDHD, encoding the cytochrome b small subunit in mitochondrial succinate-ubiquinone oxidoreductase, was isolated from the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense.

本文从青岛文昌鱼(Branchiostoma belcheri tsingtaunese)肠cDNA文库中,分离得到克隆L095和094,经鉴定它们分别编码文昌鱼线粒体琥珀酸-泛醌氧化还原酶中细胞色素b的小亚基和核糖体蛋白S27(AmphiS27)。

Methods 26 patients with intermediate or advanced pancreatic head carcinoma underwent cholecystojejunostomy combined with intraoperative radiotherapy with an electron beam on carcinoma from May 1996 to May 1998. The catheter of multifunctional implantable drug delivery system was inserted via gastroduodenal artery for postoperative perfusion chemotherapy.

对26例中晚期胰头癌患者施行了胆肠吻合术,手术中行直线加速器肿瘤区直接照射,同时手术中经胃十二指肠动脉置入埋入式化疗泵,手术后行肿瘤区灌注化疗。

The anterior border of all flaps exceeded crista anterior fibulae, and that of 2 flaps reached to the crista anterior tibiae. The width of flap on anterial compartment of the cnemis ranged from 2.5cm to 7.0cm, averagely 4.0 cm.

行前界扩大的改良腓肠肌外侧头肌皮瓣21例,修复的创面位于小腿上1/3段9例,中1/3段前内侧7例,中1/3段前外侧5例。21例皮瓣前缘均超过腓骨前缘,其中2例达胫骨前嵴,位于小腿前骨筋膜室表面的皮瓣部分宽度为2.5~7.0 cm,平均4.0 cm。

RT-PCR results showed that GDF8/11 gene was expressed in new fertilized cell, early gastrulation and knife-shaped embryo, which was different from that in mammals.

RT-PCR结果表明GDF8/11基因在新受精的细胞、早原肠胚和刀形胚胎中表达,这与哺乳动物中的情况不同。

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