中肠

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

Results:The level of total anaerobic population and total aerobic population of excrement specimens of three kinds of IBS sufferers were different significantly, particularly the level of bifidobacterium and cillobacterium(P.01);the level of bacteroid population was higher significantly in diarrhea-predominant and mixed type of IBS sufferers (P.01);the level of enterococcus population was higher significantly in diarrhea-predominant IBS sufferers(P.05);among the aerobes, the number of Escherichia coli and Staphylococcus aureus colonies were increased by different level(P.01or0.05) in the IBS suffers, in particular, the number of Escherichia coli colonies in diarrhea-predominant IBS sufferers increased most significantly.

结果：三种类型的IBS患者粪便中总厌氧菌群和需氧菌群数目与健康人差异明显，特别是双歧杆菌属和乳酸杆菌属明显减少（P.01；拟杆菌菌落数在腹泻型和混合型IBS患者中明显增加（P.01）；肠球菌菌落数在腹泻型IBS患者中明显增加（P.05）；需氧菌中患者组大肠杆菌和金黄色葡萄球菌菌落数均不同程度增加（P.01或0.05），大肠杆菌菌落数在腹泻型IBS患者中增加最明显。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

The expression level of cAPN mRNA in lung,glandular stomach and jejunum were higher than that in other tissues,and the expression level of it in bursa of Fabricius and spleen were lower than that in other.

结果显示，随着日龄增加，肝、脾、法氏囊、回肠、肾等组织中的cAPN表达量相对稳定，肺、腺胃、空肠等组织中的cAPN表达量呈现持续下降趋势，而十二指肠中的cAPN表达量持续上升，但盲肠、空肠中的cAPN表达量规律性不强且波动较大。

This may be due to hydrolytic chelating, precipitation and the sediment or hydrobioes attachment, absorption etc. The honewort had stronger ability of accumulating La because of its large leaf surface that could attach La. The accumulating ability of snail and fish to La was relatively weaker but some organs of them had higher contents of La especially in the intestines, shell of snail, and in gill, internal organs, scales, head, bone of fish, rare in muscle. The total accumulating quantities of La in each component of simulated aquatic ecosystem were a decreasing order of: sediment ＞honewort＞snail＞fish, and the sequence of bio-concentration facter was as follows: honewort＞snail＞fish.

2金鱼藻对La有较强的富集能力，主要通过枝叶表面吸附、吸收而富集；螺蛳对La也有一定的富集，并主要分布于肠、壳中，螺头中积累相对较少；草鱼对La的富集能力相对较弱，进入鱼体的La主要集中在鳃、内脏、鳞及头、骨中，肌肉中积累量较少；按质量计，稀土元素La在模拟水生态系统各部分中的积累量依次为：底泥＞金鱼藻＞螺蛳＞草鱼；各水生生物对稀土La的生物富集因子依次为：金鱼藻＞螺蛳＞草鱼。

GFP expression started during gastrulation in the axial hypoblast layer. During segmentation, GFP was detected in the notochord but not in the foor plate.

GFP在原肠作用期就开始表达，主要发生在中轴下胚层中；在体节形成期，GFP在脊索细胞中表达，但未发现在神经底板细胞中表达。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

With the development of embryo, Myf5 expression decreased gradually in somites in the anterior region, but remained strong in the newly formed somites; After 30 somites formed, MyoD expression decreased in the somites except the caudal somites. At the hatching stage, MyoD and Myf5 were expressed in head muscle cells and fin muscle cells. In the growing fish, Myf5 was expressed in the skeletal muscle and intestine, and in adult flounder, Myf5 was only expressed in muscle. In the growing fish and adult fish, MyoD was only expressed in muscle.

在胚胎发育早期，Myf5在近轴中胚层中表达，体节发生过程中，Myf5在体节中表达，MyoD基因最早在分节板的体节前细胞中表达，随后在近轴细胞、体节中表达；随着胚胎的发育，Myf5在成熟体节中表达量降低，在新生体节中表达较强；MyoD自30个体节时期后只在新生的尾部体节中表达，在成熟的体节中表达量降低；在孵化期，MyoD和Myf5在头部及鳍的肌肉、尾部的体节中表达；生长期的牙鲆中，Myf5在骨骼肌和肠中表达，成体牙鲆中，Myf5只在肌肉中表达；生长期的牙鲆及成体牙鲆中，MyoD只在肌肉组织中表达。

The ultrastructure was observed by electrical microscope and the specimens were collected from bowel at pull-through operation on 4 HD patients.

对照组结肠取自结肠癌患者及肠套叠患儿行肠切除吻合术手术切除的标本，这些人均无先天性肠功能异常的病史，取标本中正常的组织作为正常对照。

However, not all herbivores have digestive systems that maximise fibre digestibility; only ruminants, camelids and very large hindgut fermenters achieve this.

对比而言，许多其它的食草动物，如前肠发酵的有袋类和小型的后肠发酵动物如兔子、田鼠和负鼠等，它们具备可以使植物纤维消化效率最大的消化系统，可以在食物中的纤维素含量非常高的情况下仍能处理大量的食物。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。