中箱

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

METHODS The residual concentrations of sodium formaldehyde sulfoxylate in the solutions were determined by iodimetry, the datas were fitted by linear regressions and then ...

在高精度电热恒温箱中在不断通入空气或医用纯氧的情况下，采用碘量法测定甲醛合次硫酸氢钠在水溶液中不同时刻的浓度，作出降解曲线，将测得的数据进行降解动力学方程的拟合并获得动力学参数，并计算反应速率常数。

Take Wasatch Core Growth WGROX and Brazos Microcap BJMIX, for example. Both land in the small-cap value portion of the style box. Yet Wasatch's median market cap at the end of August 2002 was over $1 billion, while Brazos' was just $384 million.

以WGROX基金和BJMIX基金来举例，这两只基金都处在风格箱的小盘价值区，然而WGROX基金在2002年8月末的中位市值超过10亿，而与此同时，BJMIX基金的中位市值只有3.84亿，其间差别甚大。

Consolidation test is a important link of obtaining mechanical properties index of soil and coefficient of compressibility gained through the test is a key data to evaluating the compressibility of soil. Consisting of solid phase and liquid phase of the salty soil is different from the non-salty one. Eutectic salt in soil may exist as liquid phase, solid phase or both of them and these phases can interconvert because it is affected by temperature, Na_2SO_4 content, water content. Especially when temperature drops, Na_2SO_4 will crystallize and expand, namely salt expansion of soil. These particularities make soil sample put in environmental cabinet when consolidation test is going on. So soil sample can be compressed by temperature controlling system under a set temperature.From experiment, the formulae counting coefficient of compressibility of salty soil have been given.

土的固结试验，是取得土的力学性质指标的一个重要环节，因为土的固结试验所取得的压缩系数，对评价土的压缩性是一个关键数据，但由于盐渍土的固相、液相组成和非盐渍土不同，土中易溶盐受温度、含盐量、含水量的影响可以以液相、固相、液相和固相混合形式存在，且它们之间的存在形式可以相互转换，尤其是当温度下降时，硫酸钠便结晶产生体积膨胀，即盐胀，这些特殊性就使得在进行硫酸盐渍土的固结实验时，须将土样放置在环境箱中，通过温度控制系统使土样在设定的温度下受压产生变形。

The mixture was added to islets medium,and cultured at 37℃in a humidified atmosphere of 5%CO_2 and 95%air for 8 hours.

将siRNA-转染试剂混合液加入含有胰岛的培养基中，在37℃的CO_2培养箱中培养8h后将培养基更换为含有血清和抗菌素的完全培养基，继续培养24、48h。

Finally, through the instance of engineering, we can see that the steel dosage in steel honeycomb plank is smaller and the self-weight of the whole structure is lighter comparing with pyramided grid structure, and the steel dosage in portal strut with honeycomb laminated beam-column system is still smaller and the self-weight is still smaller comparing with steel I-beam,they will have a wide applied foreground.

最后，通过工程实例分析表明，在大跨度屋盖结构中，金属蜂窝板-杆空间结构体系比常规的四角锥网架结构具有用钢量低，结构整体自重轻等优点；在轻钢结构厂房中，采用金属蜂窝纯板片箱梁体系比一般H型钢结构用钢量更低，自重更轻，具有广泛的应用前景。

The range ratio and phasic difference of concrete box-girder temperature to daily temperature is quantitated.

在温度作用下，3跨变高度预应力混凝土连续箱梁的最不利位置位于中跨跨中截面的顶板下表面处，该位置的温度作用效应最大。

Properties : The working principle of air conditioner is that air refrigerates in the evaporating box through circulation.

性能特点：空调的使用原理是让环境中的空气在蒸发箱中循环得以制冷。

Transporting the partially stoppered containers to the lyophilizer and loading into the chamber under aseptic conditions.

在无菌条件下转移半加塞的容器到冻干机中，放置在前箱中。

And in closed-loop environments, reusable plastic totes and pallets in automated storage systems help reduce the amount of transport packaging that wind up in landfills.

而在一个闭环的环境，在自动存储系统中可重复使用的塑料零件箱和托盘也有助于减少运输过程中的需要填埋风化的包装量。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。