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METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上,剪开大鼠颅骨,显露位于颅腔前方的嗅球,取出2只嗅球,在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织,保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层,剪成1 mm3小块分离获取单细胞悬浮液,调整细胞密度至1×107 L-1,接种在用poly-l-lysine包被的培养瓶或培养板中,于37 ℃、体积分数为5%的CO2培养箱中培养,第3天用无血清DMEM/F12培养基换液培养。

You can find out it is the major temporary work, such as formwork, rebar jig, equipment etc. during the FPLM construction, in which the cost of the inner mould and outer form of box girder, the steel form of pier and pierhead of substructure, the rebar jig as well as the launching equipments are quite high. The quantity of those equipments was always depended on experience in the pass time. Therefore the more of the form quantity is used the higher of direct costs would be spent. On the other hand it would be reduced the capacity of production if the equipments are insufficient.

在全跨预铸工法中所使用的生产设备仔细观察可发现,预铸工法中假设工程(如模版、钢筋加工架以及施工机具等)所占比例相当高,其中有箱型梁的内模与外模床、下构的柱钢模、帽梁钢模、钢筋加工架、吊装机具等所占的成本相当高,以往设备数量多寡均以经验来取决,因此假如模版数量过多直接成本随之提升,反观模版资源数量不足时又会造成生产力降低情况发生。

In the system, the management of sales confirmation, invoice, packing list and list of stock with draw merge into an organic whole.

在本系统中,药品出口业务中的合同、发票、箱单和出库单等单据的处理实现了一体化流水作业,而打印、查询和统计等功能也都很完善。

Vacuum sweeper with robust petrol engine for outdoor use. Height-adjustable roller brush, handy mobile waste container. Dust created during sweeping is suctioned in and retained by a large filter. Mechanical filter shaker.

汽油机驱动手推式自走吸尘清扫车;特别适合室外用;配备强劲的本田汽油发动机;高度可调整式主刷;浮动式边刷;移动式尘箱;清扫过程中所产生的尘埃被及时吸入并保留在过滤器中;机械式过滤器除尘系统。

The optimum hydrolytic condition for crude drug was: zymolysis at 39℃for 72 hours, hydrolysis at 90℃with 5% sulfuric acid for 6 hours under natural pressure; The reserved time of diosgenin was about 10 minutes under its chromatograms conditions, and the R-value between the main peak and the nearby peak was bigger than 2, which means reaching the standard of chromatograms separation.

生药材的最佳水解条件是:在39℃恒温箱中发酵72 h,加硫酸至5%,90℃水浴中常压水解6 h。

The Dongping Bridge is a new arcuated built-up system bridges. Half through steel box tied arch and prestressed concrete continuous beam were connected directly, and then the bridge becomes the Cooperation System Bridge with vice-arch.

东平大桥是一种新型的拱梁组合桥,由中承式钢箱系杆拱与预应力混凝土连续梁直接连接形成协作体系,并保留施工过程中的拉杆形成副拱。

Using the static chamber and Chromatograph method, H2S and COS emission from the marsh meadow Calamagrostis angustifolia and typical meadow Calamagrostis angustifolia were measured during growth season (5-9 month). the results showed that the seasonal and diurnal variations of H2S and COS emission were both obvious, the mean emission of H2S and COS from the marsh meadow Calamagrostis angustifolia were 0.34μg?

利用静态箱/气相色谱法,观测了小叶章沼泽化草甸和小叶章典型草甸两种湿地类型中H2S和COS在生长季(5-9月)的释放动态,结果表明:在两种小叶章湿地中,H2S和COS的排放通量均具有明显的季节和日变化规律。

The paper introduces the characteristics and performance of HGIS devices and their application in Bao'an Converter Station of GuiGuang II DC Project,and analyzes the abnormal condition that a 500 kV HGIS disconnector did not work correctly due to the difference between its framework and blueprint.Based on the analysis of the original blueprint,three design alternation schemes are presented and compared to compensate the mismatch,and the realization of a scheme in this station is discussed.In addition,some suggestions about design,commissioning and operation of an HGIS are proposed.

笔者首先简要介绍了HGIS设备的特点、性能以及国产500 kV HGIS开关设备在贵广II回直流工程宝安换流站的应用情况,接着分析了因隔离开关机构箱与设计图存在一些差别导致现场调试过程中出现隔离开关不能正常操作的情况,在分析原始设计图的设计原则的基础上,给出了3种设计变更方案及其优劣性对比,介绍了该站所选方案的具体实现情况,最后给出了在今后的HGIS设计、调试和运行过程中须注意的几点建议。

Research Methodology will help you to complete your dissertation, to prepare future doctorial study and to plan practical business strategy.

本课程将采用「接龙」「小鬼当家」「非常男女恐怖箱」「我就是科学大师」「我就是领袖」等启发式教学活动,激发你在生活中学习,在互动中开发你的潜能。

In the six-screen projection, Hako (2007), Sawa subtly transforms images of a Shinto monastery, a nuclear power station and various landscapes and interiors by appropriating readymade objects or software and inserting them into otherwise ordinary scenes.

在他的六屏幕投影作品&箱&(2007)中,通过数码手段挪用现成对象,巧妙的将神道寺、核电站、景观等图像进行改变,再将其嵌入到普通的场景中,因此产生了现实与幻想之间微妙地转换,人们记忆被模糊化的效果。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。