中种被

The invention relates to a method for the extraction of keratin from dead animal skins, characterised in the treatment of dead animal skins with a substance of general formula, or at least one corresponding alkali metal, earth alkali metal, ammonium, or phosphonium salt, the variables having the following definitions: R, R = together or independently, H, C6-C14 aryl, C1-C12 alkyl, unsubstituted or substituted with one or several SH or OH groups, R, R = together or independently, H, C6-C14 aryl, C1-C12 alkyl, unsubstituted or substituted with one or several SH or OH groups, whereby at least one of R or R is not H, or R and R are not H and two vicinal groups of R to R together may be C3-C10 alkylene, R = H, C1-C12 alkyl, H-C=O or C1-C4-Alkyl-C=O, X, X, X and X = OH, SH or NHR, whereby where Rto R contains at least one sulphur atom, at least one of X to X = SH and where R to R contains no sulphur atom, at least two of X to X= SH.

本发明涉及一种从死亡动物原皮中除去角质的方法，其特征在于用通式I的物质或至少一种对应的碱金属盐、碱土金属盐、铵盐或∴盐处理死亡动物原皮，式I中各变量定义如下：R 1 和R 4 相同或不同且选自氢、C 6 -C 14 芳基和C 1 -C 12 烷基，其未被取代或被一个或多个SH或OH基团取代；R 2 和R 3 相同或不同且选自氢、C 6 -C 14 芳基和C 1 -C 12 烷基，其未被取代或被一个或多个SH或OH基团取代，其中至少一个基团R 2 或R 3 不为氢，或R 1 和R 4 不为氢，且两个相邻基团R 1 -R 4 可以一起为C 3 -C 10 亚烷基；R 5 选自氢、C 1 -C 12 烷基、H-C＝O或C 1 -C 4 烷基-C＝O；X 1 、X 2 、X 3 和X 4 选自OH、SH和NHR 5 ，其中若R 1 -R 4 含有至少一个硫原子，则至少一个基团X 1 -X 4 为SH，以及若R 1 -R 4 不含硫原子，则至少两个基团X 1 -X 4 为SH。

We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.

结果表明：花背蟾蜍蝌蚪(1)具有晶状体再生的能力，其晶状体的再生来源于虹膜背缘的上皮细胞；(2)虹膜背缘去色素是转分化的前提，主要有两种方式：一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞，巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒；另一种方式是虹膜色素上皮细胞自身释放色素颗粒；(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化，核糖体增多，粗面内质网增多，晶状体纤维分化过程中细胞核逐渐凝聚；(4)SDS-PAGE电泳发现，花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似，蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白，并且后两种蛋白几乎同时表达。

The states with their structures similar to the ground state0+1 form a ground rotation band and they all can get access to the regular triangle shape.

在有的态中仅有一种模式被激发，另一些则两种模式均被激发，而在基态中不包含任何一种激发的模式。

The single-celled phytoplankton included 116 inshore wide distribution species, 31 inshore warm-water species and 26 inshore temperate species, and the vascular plants were of 6 ecological groups, i.e., halophtes (24 species), hydrophtes (22 species), amphiphytes (35 species ), mesopllytes (103 species), zerophytes (6 species), and psammons (4 species). Halophtes, hydrophtes, and amphiphytes were the edificators and dominants, reflecting the azonal feature of the wetland vegetations. The vascular plants were divided into 5 life-forms, including 20 phaenerophytes, 4 chamaephytes, 54 hemicryptophytes, 48 geophytes and 68 therophytes, among which, hemicryptophytes and geophytes accounted for 52.58%, reflecting the relatively important function of cold and wet climate and local water-accumulated bottomland environment on the vascular flora formation.

区系中的单细胞浮游藻类包括近岸广布种116种、近岸暖水种31种、近岸温带种26种；维管束植物被划分为6大生态类群，包括盐生植物24种、水生植物22种、湿生植物35种、中生植物103种、旱生植物6种、沙生植物4种，保护区内湿地植被的建群种、优势种均为水生植物、湿生植物或盐生植物，反映了湿地植被的隐域性；维管束植物按照生活型划分，有20种高位芽植物、4种地上芽植物、54种地面芽植物、48种地下芽植物和68种1年生植物，其中地面芽植物、地下芽植物占区系植物总种数的比例较高，占52.58%，反映冷湿气候和局部低洼积水环境对区系的形成起较重要作用。

The AMF spore average density was 24.88 per 20 g soil , the species richness was 1.94 per soil sample, the species diversity index was 2.56, and the species evenness was 0.65. Glomus, but they all were of prominent genus in the investigation area and Acaulospora, Scutellospora were of common genus while Entrophospora was rarely found. In the 13 species AM fungi, G.mosseae was of prominent species, and G.geosporum, G.intraradices, G.etunicatum were of common speices, while the others were of rarely species. There were 87.5% sand fixation plant were infected with arbuscular mycorrhizal fungi, the highest spore density, the strongest infection strength of the whole roots and the best species richness belonged to Chenopodiaceae while Polygonacea was the lowest.

四属AM真菌中，Glomus为优势属， Scutellospora 、Acaulospora为常见属，Entrophospora则为稀有属；十三种AM真菌中，G.mosseae、G.geosporum分别为优势种、最常见种，G.intraradices，G.etunicatum为常见种，其余均为稀有种；五科固沙植物中，87.5%的植物能被菌根侵染，其中藜科植物孢子密度最高，整个根系侵染强度最强，种的丰度最高，蓼科植物孢子密度、整个根系侵染强度、种的丰度则最低；禾本科植物不仅具有最好的丛枝结构，且具有相对高的亲和性，能与12种AM真菌共生。

Ophyra aenescens (Wiedemann,1830),which distributed in Palaearctic,Nearctic,Australia and Neotropic Regions,but not known to China before,was recently found in Shanghai,China.It was also intercepted from waste paper in a cargo from America and Australia.The present paper deals with the diagnostics,biology,distribution and others of the species.

2001年3月在浦东国际机场附近一孔雀尸体上发现1种中国前所未见的黑蝇，被疑为近年从境外传入，该种黑蝇被鉴定为古铜黑蝇〔 Ophyra aenescens (Wiedemann，1830)〕，原产于美洲，可携带脊髓灰质炎病毒、沙门菌、志贺菌等，是一种重要的病媒性蝇类；2002～2004年在上海其他地区的调查中，也采到了该种；上海口岸于2003年12月22日从美国和2005年4月22日从澳大利亚的入境废纸中先后截获该蝇。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

Everything is portrayed in the light of instinctive emotions, and the free association which takes root in memory is serving this pure language of traces as it develops.

痕迹感与联想是截然不同的两种事物，而作品中的痕迹感，又于当时的时空中凸显了儿童想象力的无限延伸，看似无厘头的堆砌，其实质是一种原生状态的自由驰骋，没有被过多地教化，没有被&文明&思想所侵蚀，一切都以一种本能的情绪去涵盖，这种孕于痕迹感中的联想情结，在其产生的过程中就服务于那种单纯以至真的痕迹语言。

If it be shown in a reasonable number of well-authenticated cases that these conditions — exogamy as tribal law, and hostility as the prevailing relation of separate tribes towards each other — exist or have existed, accompanied, as might have been expected, by a system of capturing wives, we shall be justified in concluding — failing the appearance of any phenomena inconsistent with such an explanation — that the same conditions have existed in every case where the system of capture prevailed, or where the form of capture has been observed as a ceremony of marriage. Nothing more than this is necessary to satisfy the conditions of a sound hypothesis.We are in a position to do this and more.

并且如果它被证明在一个合理数目的被充分鉴别的案例中这些条件（异族结婚为部落法律，以及对抗是分离的部落相互对待的主要关系）存在或曾经存在，伴随着似可预期的一种猎妻制度，我们将被证明在做出结论时是正确的（舍弃与这样一种假设不协调的现象的出现）这些相同条件存在于流行劫掠制度、或者劫掠形式在婚礼中被观察到的地方的每一个案例。

"Word here but pour that gaze after but hand wield " be that "A Dream in Red Mansions" middle is various narrate to grow once in art judging from content promising fish sets up the bait cause and effect effect have the disheartened line of grass snake on structure effect vanquishing line long distance the integral and indivisible sky is accomplished as for coming up to rectify an article if floating clouds and flowing water the chisel and the vulture adorn the even a tiny bit axe of nothing Shimizu goes out of Seremban just like natural get rid of vulture ornaments But every piece of natural coagulation of thing has become one share trickling sluggishly flow the stream living is natural and aesthetic as well one by one minor livelihood branch has woven into a very dense net the domino.

言在此而意在彼是《红(yan2 zai4 ci3 er2 yi4 zai4 bi3 shi4 _hong2)楼梦》中多种叙事艺术中一种，被脂砚斋喻为&言此而注彼，目送而手挥&，从内容上看有为鱼设饵的因果作用，在结构上有草蛇灰线，伏线。。。言在此而意在彼是《红(yan2 zai4 ci3 er2 yi4 zai4 bi3 shi4 _hong2)楼梦》中多种叙事艺术中一种，被脂砚斋喻为&言此而注彼，目送而手挥&，从内容上看有为鱼设饵的因果作用，在结构上有草蛇灰线，伏线仟里的效果，从整篇文章上来看浑然天成，如行云流水，无丝毫的斧凿与雕饰，宛如清水出芙蓉，天然去雕饰，每一件事情又自然而然的凝结成了一股涓涓流淌的生活流，自然而又唯美，而各个小的生活支流又织成了一张密密麻麻的网，牵一发而动全身。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。