中柱

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

This project proposal will draw up the short-term, mid-term and long-term research plans in nine years based on the capacity of the fire lab of ABRI in Tainan and the each term covers three years. One or two research topics will be planed in each year. The planned topics will focus on studying the structural behaviors of SRC and steel composite structures in fire and investigate the important parameters affecting the mechanical behaviors in fire. The most economical experiments will be designed during nine years. This research will invite Prof. Michael D. Engelhardt from the University of Texas at Austin to participate in some research topic planning for international collaboration.

本计画主要系依据内政部建筑研究所防火实验中心梁柱楼版复合炉可执行之实验项目暨国内建筑实况，并据此来规划研拟为期9年之短、中、长程研究计画为原则，每期以3年为一期程，每年研拟1~2个研究课题，针对不同复合构件研究，以探讨分析结构火害力学行为所必要之参数，并规划进行最精简之实验，本研究拟邀请国外学者参与部分研究课题，提供与国际合作之规划内容及研究方向之建议，以使未来研究成果能与国际接轨。

For service, empty the Absinthe colum from ice and fill it with a bottle of MOET ET CHANDON champagne.

在招待时，把苦艾酒柱中的冰倒光，用一瓶酩悦香槟填充。

To assess the importance of quasar outflow we have performed a series of studies on broad absorption line quasars, and have obtained the following results.

为了确定外流的重要性，中国科学技术大学天体物理中心开展了宽吸收线类星体的一系列研究，取得了一些进展：(1)宽吸收线区的紫外吸收物质的柱密度比原来预计高出2个量级，低电离吸收线形成于饱和的高电离吸收线区；(2)射电宁静的宽吸收类星体吸收物质主要在赤道面，并且不同的源吸收物质分布情况很不相同，而在射电强的类星体中，赤道和极向外流都是可能的；(3)共振散射线偏振表明，外流物质携带角动量。

Objective: To investigate the role of the dorsal column in the inhibitory effect of somatic afferent inputs on the central pressor response.

目的：探讨脊髓背柱在躯体传入冲动对下丘脑室旁核兴奋所致的心血管反应的抑制作用中的地位。

The aftermost transverse structural member in a steel ship, including the floor, frame, and beam assembly at the sternpost.

船尾构架铁船中最靠近船尾的横向组成部分，包括在船尾柱附近的甲板、肋骨和横梁上

Device in a brass wind instrument for varying the length of the air column to alter the pitch of a tone .

黄铜的管乐器中改变空气柱的长度来变化音调的装置。

One mechanism of temperature effect is that temperature influence water characters, such as surface tension and viscidity, the other one is that temperature affect on the quantity of air in experiment column.

温度对土壤水分运动和保持的影响一方面是由于温度影响水的表面张力和粘滞性，另一方面是由于温度影响密封土柱中空气的含量。

Aiming at the frequent occurrence of drill-string fracture during gas drilling in the Puguang gas field, this paper, based on the statistical analysis of accidents on fields, makes in-depth investigation into the origins of drill-string accidents in aspects of peculiar drilling medium, different rock-breaking methods, and the vibration of drill string during the drilling process. Through macro, micro and material analysis of the fracture surface of drill string, the law of drill-string failure due to fracture is discovered and the fatigue fracture is found out to be the main type of drill-string fracture.

针对普光气田气体钻井过程中存在的钻具断裂频繁的问题，在对现场事故统计分析的基础上，从特殊的钻井介质、不同的破岩方式以及钻井过程中钻柱共振等方面定性分析了钻具断裂的原因；通过对钻具断口宏观、微观及材质分析，找到了钻具断裂失效的规律和主要形式－疲劳断裂。

In comparison, emulsions of hexanoic acid produced a slight elongation of the normal prismatic aqueous morphology in 100% supersaturated systems, with little discernible growth modification in the 200% supersaturated systems.

相比之下，己酸乳液在100 ％过饱体系中的正常棱柱水溶液形态产生了轻微的拉升，而在200 ％过饱体系中表现不明显。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。