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中期分裂

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The result showed that the plant of Maicao No. 9 was tall, with good fecundity, fully filled grain and vigorous growth. The chromosome number of the root cells was 2n=56, through the observation of pollen mother cells meiosis, there were a certain amount of univalents, ring bivalents and bar bivalents in PMCM. Different numbers of lagging chromosomes, chromosome bridges and micronucleus were found in anaphaseⅠ, anaphaseⅡ and tetraspore.

结果表明:麦草9号植株高大,结实性较好,籽粒饱满,生长旺盛;根尖细胞染色体数目为2n=56,对其进行花粉母细胞减数分裂观察,中期Ⅰ具有一定数量的单价体、环状二价体、棒状二价体;后期Ⅰ、后期Ⅱ与四分体时期都出现不同数量的落后染色体、染色体桥、微核等现象。

The frequency of cells with univalent at MI and with lagging chromosomes and chromosomal bridge at A I in PMC of F1 hybrids of 4 alloplasmic 1BL:/1RS and non-1BL/1RS wheat male sterile lines with Aegilops kotschyi,Ae. variabilis,Ae.ventricosa and Ae.bicornis cytoplasms were systematically investigated,and the relationship between FCU at M I,FCLB at A I and F1 selfed seed set was analyzed.

系统调查了4类异质(粘果、易变、偏凸、二角山羊草细胞质)1BL/lRS、非1BL/1RS小麦雄性不育系与其恢复系杂种F减数分裂中期Ⅰ出现单价体细胞频率,以及后期Ⅰ出现落后染色体和染色体桥细胞频率,并对中、后期染色体变异率与杂种F自交结实率进行了相关分析。

The expression profiles analysis of 25 genes belonging to 8 types in different development periods of Y35 somatic embry showed, genes involved in transcription, signal transduction, cell fate, substance transport, cell and development regulation were active at early and middle stages of somatic embryogenesis, while at the stage of cotyledonary embryo maturation, the high level-expressed genes were mainly those related to storage protein, maturation and defense.

通过半定量和Real-Time PCR技术,对WRKY和MAPKK等8类25个差异表达基因在Y35体细胞胚不同发育时期的表达分析显示,转录、信号转导、细胞生长分裂、细胞命运、物质转运和多数发育调节相关基因在体细胞胚胎发生的早期或中期比较活跃,而进入子叶胚成熟后期则主要是储藏蛋白、成熟和防御相关基因大量表达。

Fluorescence in situ hybridization and double FISH experiments were carried out to ascertain the chromosomal distribution patterns of the 45S and 5S ribosomal DNAs in the three species of Cucurbitaceae.

首次利用荧光原位杂交和双色荧光原位杂交技术对45S和5S rDNA在南瓜、丝瓜、冬瓜的有丝分裂中期染色体上进行了物理定位分析。

By phenotype observation, combined with root tipchromosome counting, PMC MI pairing analysis and chromosome C-banding, a ditelosomic addition line 94K227 and a disomic addition line 94K280 was identified among BC2F2 and BC2F3 population respectively.C-banding results showed that 94K227 contains 1 pair of long arms in chromosome B, and 94K280 contains 1 pair of chromosome D from R.

用普通小麦-纤毛鹅观草双倍体与普通小麦中国春连续回交两次,然后连续自交,通过形态观察、根尖细胞染色体计数、花粉母细胞减数分裂中期Ⅰ染色体配对分析及染色体C-分带,在BC2F3和BC2F3群体中,分别筛选到一个端二体异附加系94K227和一个二体异附加系94K280,其中C-分带显示94K227添加的是纤毛鹅观草染色体B的一对长臂, 94K280添加的是纤毛鹅观草的一对染色体D。

It shows that As2O3 has marked cytogenetic toxic effect on the root tip cells of Vicia Faba,and effect of accumulating the metaphases in mitosis.

结论是As2O3对蚕豆根尖细胞具有明显的细胞遗传学毒性效应,并具有积累有丝分裂中期细胞的效应。

Fromthese facts,It could be deduced that 8 to 14 pairs of chromosomes possessed a certaindegree of hetorology,which was directly groved to be hybrid origin of P.tomentosaaccording to the good chromosome homology and fertility of P.bolleana and thehybrids of P.tomentosa×P.bolleana,it could be further deduced that the original parentsof P.tomentosa involved probably P.bolleana or P.alba.

从终变期、中期I的染色体构型看,有数目不等的联会程度较差的单价体和棒状二价体出现,在后期I,甚至末期I亦可见到含落后染色体分裂相的细胞,由此推断可能有8~14对染色体表现出一定程度的异源性,鉴于新疆杨以及毛白杨与新疆杨杂交产生的杂种毛新杨的染色体同源性较强、育性较好等现象,进一步推断毛白杨起源亲本可能包括新疆杨或银白杨。

Methods Cell cultures of8HNSCC cell lines were stained with haematoxylin and eosin,to analyze mitotic cells with anaphase bridge morphology.

用荧光结合 3 核酸探针以荧光原位杂交方法检测细胞分裂中期染色体末端端粒表达,计算平均每细胞中端粒缺失染色体末端。

In this study, the variability of number of telomere DNA in maize genome was discussed, we have located and analyzed the sites of the 45 S and 5S rDNA on the mitotic metaphase chromosomes of barley (Hordeum vulgare L.).

的有丝分裂中期染色体进行了确定分析,较强的45S rDNA信号共有两对,分别分布在大麦的第1染色体的短臂和第2染色体的长臂。

Confocal laser scanning microscopy used to localize the hsp70 protein in oocytes and embryos revealed that the distribution of hsp70 in the ooplasm of immature and mature oocytes is unaffected by exposure to decreased or elevated temperatures and that this protein was closely associated with the meiotic spindle indicating its possible role in stabilizing this structure, hi eight-cell embryos derived under control conditions, hsp70 was evenly distributed in the cytoplasm but appeared as aggregates in some embryos exposed to elevated temperature.

此蛋白均匀地分布在两种细胞的胞浆中。此形态特点与减数分裂期纺缍体有关,由此表明hsp70在中期Ⅱ期,具有稳定这种结构的作用。在对照组8细胞胚中,hsp70均匀地分布在胞浆中,而在处理组一些胚胎内,hsp70则发生凝集。

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这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。