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RESULTS AND CONCLUSION: The seeded cells had chondrogenesis in scaffolds under certain conditions.

结果与结论:种子细胞在三维支架中特定条件下有成软骨效应,骨髓间充质干细胞在含20%透明质酸的胶原/透明质酸支架扩增少于3代有成软骨效应,软骨细胞传代数更高仍然有成软骨效应,但脂肪源性成熟基质细胞成软骨效应能力较弱。

Further, with a semi-quantitative RT-PCR technique, the temporal expression of UuMAPKKK-like in U. unicinctus coelomic fluid cells was measured after stimulated by sulfide. The mRNA transcript of UuMAPKKK-like was low in the control and short time stressed (2 h, 6 h) groups, up-regulated gradually after 12h stimulation, and then reached its maximum level at 48h.

进一步采用RT-PCR技术对其在硫化物刺激前后的表达进行了检测,结果显示该基因在对照和应激后2h、6h的个体中表达较弱;刺激12h后表达量增高,并随应激时间增加,呈明显上调趋势。

An amount increase ofPAI-3 andK10 was observed after theKCswere cultured for48 h in high calcium medium,butat72 h the exprssion ofPAI-3 decreased. The positive stain ofPAI-3 wasmainly localized in perinuclearmembrane in lowcalcium medium and in cytoplasm in high calcium medium. PAI-3 was incorporated into cornified envelope ofterminally differentiated keratinocytes.

结果 高Ca2+浓度下培养24 h后,分化标记物K10弱阳性表达, PAI-3呈阳性表达;培养48 h后, K10强阳性表达,而PAI-3的阳性表达明显增加, 72 h后,表达开始降低,在低Ca2+浓度下阳性染色主要位于细胞核膜周围而高Ca2+浓度下主要位于细胞质,同时, PAI-3存在终末分化KCs角质壳中。

Knoevenagel condensation reaction of aromatic aldehydes with active methylene compounds like ethyl cyanoacetate proceeds smoothly in water in the presence of 8mol% of diammonium hydrogen phosphate and 2mol% TEBA.

醛与活泼亚甲基化合物的 Knoevenagel 反应是有机合成中广泛应用的形成碳碳键的重要方法,该反应一般是弱碱性催化剂存在下完成的,如各类胺、脲[1]等,Lerwis酸[2]、杂多酸[3]、氨基酸[4]等也可用于催化该反应。

Although ERβ was also seen in these cells,there was irregularity of its distribution.Weak to moderate cytoplastic staining of ERβ in epithelial cells of lobules and ducts were fonud.

ERβ在小叶上皮细胞和导管上皮细胞的细胞核内检测到,但是ERβ在上皮细胞中的分布并无规律,而且在细胞浆内也可以检测到弱到中等强度的染色。

Wound healing of rat skin may be arbitrarilydivided into the following stages: wound generation, suppurating in someinflammation area, growing of granulation tissue, scab formation,decrustation, and completely healing. The wound depth and the extent ofhealing also could be determined from the OCT images in the different healingtime. Healing time for the skin scald was found to be much shorter than those ofthe skin wound. Because the skin wound is serious than the skin scalds. 3 The result of using OCT to monitor the model of acetic acid gastric ulcer in rats:Results of this experiment showed that using OCT technology can identify theulcerative gastric mucosa tissues with normal tissues, make the imaging on theunder-layer of gastric mucosa tissues, and evaluate the healing period and healingquality. From the OCT images can be clearly found the acetic acid gastric ulcer.

3大鼠胃溃疡模型的OCT成像检测结果:实验结果显示利用OCT能够实现溃疡组织的成像,可显示正常胃壁组织的层次性结构,对于溃疡病变过程中可对黏膜下病变的深度与状况进行成像分析,从图像可以很明显的发现溃疡性炎症病变区域(这个区域由于散射光变弱,颜色相对比较暗),在愈合的后期黏膜层基本愈合的状态下仍可显示黏膜下层组织的增生、未能完全愈合等病理改变。

All the 16 clones remained from tube seedlings of Dendrocalamus latiflorus from 3-year cultivation on medium in a high concentration of BA ocoue in flowering phenomenon,and their flowering timing, earlier or later, are correlated with their viabilities, i.

麻竹试管苗经3年在高浓度BA的培养基中培养,保存下来的16个无性系都出现开花现象,开花迟早跟无性系的繁芽能力有关,繁芽能力强的无性系较早开花,繁芽能力弱的无性系较迟开花。

In this study,diallyl sulfides in garlic powder were identified via the introduction of Ag induced-ionization.

烯丙基硫化物由于弱极性不电离,本实验利用Ag+柱后诱导电离对大蒜粉剂中的含硫化合物进行质谱定性,并以二烯丙基三硫醚为参比,对各种二烯丙基含硫化合物进行定量测定。

According to different acidity of free Flavonoids in Resina Draconis, different acidic lavonoids were extracted with the pH gradient extraction method from ethyl ether.

根据血竭中游离黄酮存在着酸性差异,采用溶剂除杂,转溶于乙醚,再用pH梯度萃取得到不同酸性的黄酮,进而对弱酸性组分分离纯化得到2个单体化合物。

In aseismic design of reinforced concrete structures," strong shear weak bending " is an important design conception to guarantee the ductibility of the structure.

在钢筋混凝土结构抗震设计中,&强剪弱弯&是保证结构延性的一个重要设计概念。

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What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我,一个心中布满虚荣的女孩子真实的想法。

Oh, and I bought myself a new laptop," he said."

哦,我还给我自己买了一个新笔记本"他说。"