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Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物;培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感,据此可在传代过程中将二者分离纯化,获得纯细胞系;混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞;再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞,经过2~3 代,即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物;培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感,据此可在传代过程中将二者分离纯化,获得纯细胞系;混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞;再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞,经过2~3 代,即可得到纯化的乳腺上皮细胞系。

But in practical operating, overvoltage caused by reignition of the vacuum switching device frequently appears, and it result in damage of the capacitor bank or the switchgear itself. Ensuring the switchgear against reignition and installing a protective device are two main approaches to avoid the phenomenon. In this paper, a study on overvoltage caused by reignition due to capacitor bank switching with the vacuum switching device and its preventive measures are carried out. The main work which has been done and researches are as follows: 1. The producing mechanism of overvoltage and its influence factor are analyzed. It is pointed out that single-phase reignition is the main reason for damage of the switchgear and two-phase reignition is the main reason for damage of the capacitor bank,so overvoltage of single-phase reignition and two-phase reignition are the emphases of prevention. 2. Analysis of the special working condition of the operating switch for capacitor bank is carried out. It indicates that the vacuum switching device is difficult to realize no-reignition under this operation condition, so it is more practical to adopt protection devices. 3. Analyses of protection effect of RC and MOA and their shortages are carried out. It shows that the two methods can only restrain effectively overvoltage caused by single-phase reignition, and they cannot protect the capacitor bank when reignition of two-phase occurs. 4. The operation principle of G-R damping device is analyzed and discussed emphatically.

本文针对真空开关投切电容器组时因重燃而产生的过电压及其防治措施进行了研究,所开展的主要工作和研究结果如下: 1、对过电压的产生机理及其影响因素进行了分析,指出单相重燃是导致开关设备损坏的主要原因,而两相重燃则是导致电容器组损坏的主要原因,因此单相重燃和两相重燃过电压是防护的重点; 2、对电容器组操作开关的特殊运行条件进行了分析,指出在这种运行条件真空开关难于实现不重燃,因此采用保护装置更具有现实意义; 3、对RC与MOA的保护效果和存在的不足进行了分析,指出这两种方法均只能有效抑制单相重燃过电压,而不能在发生两相重燃时保护电容器组; 4、重点对G-R阻尼装置的工作原理进行了分析和讨论,指出该装置接入后线路中将会出现两种频率的高频振荡,而电容器组上的过电压是由这两种振荡共同决定的; 5、根据对上述两种振荡的分析指出存在一个最佳电阻值使得过电压最低,并提出了电容器组电压和最佳电阻值的近似求解方法; 6、使用电气暂态分析软件MATLAB对G-R装置的保护效果进行了仿真计算,证实该装置确实可以有效抑制重燃时的电容器组过电压; 7、根据仿真与近似计算结果的偏差提出了近似计算的修正方法,经过修正后的过电压计算结果与仿真结果吻合较好; 8、对G-R装置中放电装置G与阻尼电阻R的要求进行了分析,指出采用编织电阻和真空间隙可能是最佳选择。

And Helmuth Greiner,keeper of the official OKW operations staff war diary until 1943,seized the opportunity in 1945,when asked by the Americans to retranscribe his original notes for the lost volumes from August 1942 to March 1943,to excise passages which reflected unfavourably on fellow prisoners like General Adolf Heusinger-or too favourably on Hitler;and no doubt to curry favour with the Americans,he added lengthy paragraphs charged with pungent criticism of Hitler's conduct of the war which I found to be missing from his original handwritten notes.

还有负责保管1943年前国防军最高司令部参谋部战时运营日记的赫尔穆特·格赖纳,在1945年,当美国人让他从他保管的原始档案中重新转录从1942年8月到1943年3月间丢失的那部分记录时,他抓住了机会去除了对阿道夫·霍伊辛格中将等同伴们明显不利的部分来保护这些人——或者去除了某些对希特勒太有利的部分以取悦美国人。

The adoption of hot central plant recycling technology will reutilize pavement materials, and also be a burning issue lying before the researchers and engineers.

在沥青路面的维修、改扩建过程中将产生大量的废旧沥青混合料,以往是将其进行废置处理,这不仅会造成资源浪费,也会占用大量土地。

Incorporating the following ten tips into your Scrin play will greatly increase your effectiveness in your play.

将下面的十条技巧融入你的战术风格中将会很大程度上使你的部队在游戏中更加有效力。

He was a short, florid man, normally very fat, now very thin, the skin of his paunch hanging slackly in folds.

中将一直是个脸膛红润的矮胖子,现在却憔悴得厉害,过去的大肚皮瘦得只剩松弛打摺的难看皮囊。

The three cities areas have their own features, we choose the creative city 3 plus 3 model in the three cities to expatiate their current situation. At the same time, we both hope more and more people pay more attentions on the culture of the city, design something related with the creativity, find the best way to develop the city and make the city as a new homestead of the visualizing and spiriting.

专题中将分析他们的建筑改造手法,在对原有的历史文化遗留进行保护的前提下,对原有的工业厂房进行重新定义、设计和改造,带来的是对于建筑和生活方式的创造性理解的观念。

Several new items can be found at various vendors which do double and triple damage to players of a specific class or race. Well add more each patch, but right now Gnome Stomper, Ancient Warlock Destroyer and the Roguebane are immediately available.

商人会出售能够对于特定种族或职业的玩家造成2倍,3倍伤害的物品……以后的补丁中将加入更多,可以肯定的是侏儒震荡器,上古术士摧毁者,盗贼禁锢者将会在这个版本中出现。

This information should be used to strategize about the approach to take in subsequent interviews.

这些信息应该用来为后续访谈中将采用的方法制定策略。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。