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METHODS: The total RNA was obtained by TRIzol from an antiricin neutralizing monoclonal antibody hybridoma cell named 4C13 and heavychain and lightchain variable fragments were amplified by overlap PCR and accepted by Genbank of NCBI. VH and VL were linked by3 with a direction of VHlinkerVL and cloned to pCDNA3.1 vector expression system.

用TRIzol提取抗ricin中和性单抗杂交瘤细胞4C13总RNA,扩增其轻、重链可变区基因,登陆GenBank;用Linker3将VH和VL连接,用重叠延伸PCR克隆入真核表达载体pCDNA 3.1,用脂质体转染293T细胞,对瞬时表达产物进行生物学活性检测。

A series of antimony mercaptide compounds which formula are Sb 〓 and Sb S (CH〓〓COOR 〓(R represents an alkyl or aryl, aralkyl, n=0~2) have been synthesized. A new synthetic method has been invented to solve the defect of abroad patent method which has complex technological conditions and singleness intermediate compounds. A new catalyst QL-1 and preactivated antimony trioxide and other means have been used to avoid neutralizing, washing, separating and other procedures of intermediate and simplifing process flow at the same time. According to this new technique, a series of antimony mercaptides and their intermediate products have been synthesized with high yield.

合成了结构为SbS(CH〓〓COOR〓及Sb〓(R为烃基或芳烷基,n=0~2)的一系列硫醇锑化合物,针对国外专利报道的合成工艺存在着工艺条件苛刻,同时需多步完成,操作复杂,而且中间体品种单一的缺陷,发明了一种一步法合成硫醇锑化合物的新工艺,采用QL-1催化剂,且将三氧化二锑预先活化,变固—液相反应为液—液相反应,同时可免除中间体的中和、洗涤、分离等工序,工艺流程简化,产品收率高,且能制得系列硫醇锑产品。

The classroom is the place where language learners interact with one another in the process and asocial environment of interpersonal communication.

课堂是地方语言学习与他人交往过程中和人际沟通的反社会的环境。

And he said to them,"Have you never read what David did, when he was in need and was hungry, he and those who were with him: 26 how he entered the house of God, when Abi'athar was high priest, and ate the bread of the Presence, which it is not lawful for any but the priests to eat, and also gave it to those who were with him?"

耶稣对法利塞人说:「你们从未读过:达味在急迫中和同他一起的人,在饥饿时所作的事吗?当厄贝雅塔尔作大司祭时,达味怎样进了天主的殿,吃了除司祭外,谁也不许吃的供饼,并且还给了同他一起的人?

Before, during,and after exercise,atrail natriuretic peptides,aldosterone and arginine vasopressin,change in plasma volume and AVP were higher in AM than PM.

分别在运动前、中和休息期间测定血浆心钠素、醛固酮、精氨酸加压素、血红蛋白、红细胞压积,血、尿渗透压和血、尿钠、钾离子等,同时测定血浆容量和体重的变化。

Calcium pimelate, calcium azelate and zinc azelate are prepared by alkali neutralization process, and calcium dimer are prepared using double decomposition reaction, the effect of binary carboxylate heat stabilizer on PVC thermal stability is studied adopting Congo red method and toast with heat method.

通过碱中和法制备了庚二酸钙、壬二酸钙、壬二酸锌,采用复分解反应法制备了二聚酸钙,采用刚果红法与热烘法研究了二元羧酸盐热稳定剂对PVC的热稳定作用。

Here, we presented a method to bacterially express the major structural protein L1 of Human Papillomavirus type 18 (HPV18) as soluble form. We found that the purified L1 could self-assemble to virus-like particles.

利用大肠杆菌表达系统可溶性表达人乳头瘤病毒18型(HPV18)L1蛋白,经过纯化和重组装过程获得HPV18病毒样颗粒,研究其免疫原性和诱发中和抗体生成的水平。

The author in recent years used the traditional Chinese medicine phlegm hot clear treatment acute pneumonia,the research indicated that,the refrigeration antidote in vitro bacteriostasis,the sterilization,the neutral bacteriotoxin and the anti-viral function effective concentration was in vivo achieves with difficulty,its treatment function was the anti-infection,the anti-inflammation,the disintoxicating,the promotion organism immunity function common result.

笔者近年来采用中成药痰热清治疗急性肺炎,研究表明,清热解毒药在体外的抑菌、杀菌、中和细菌毒素和抗病毒作用的有效浓度是体内难以达到的,其治疗作用是抗感染、抗炎、解毒、促进机体免疫功能的共同结果。

Three hydroxyapatite powders (HAP1, HAP2 and HAP3) were prepared by hydrolyzation process using brushite, precipitation method using calcium hydroxide and ammonium phosphate and neutralization method using calcium hydroxide and phosphoric acid, respectively. Hydroxyapatite/collagen composite was prepared by in situ precipitation method using calcium hydroxide, phosphoric acid and bovine collagen which was extracted from the fresh bovine tendons.

本研究以相关钙盐、磷酸盐为原料,分别用二水合磷酸氢钙水解法、氢氧化钙-磷酸氢二铵沉淀法和氢氧化钙-磷酸酸碱中和工艺制备了三种羟基磷灰石粉体,分别为HAP1、HAP2和HAP3;并以氢氧化钙、磷酸和从牛腱中提取的胶原为原料通过原位沉淀制备了羟基磷灰石/胶原复合材料。

Given the current complexity of industrial recovery of 3'-methyl-2'-butenyl 3, 3-dimethyl-pentenoate and the decomposition problems with 2-methyl-1-butene, research was conducted on a process for direct recycling of MBDP involving an ester exchange reaction of MBDP with either methanol, trimethyl orthoacetate, or solutions of methanol and trimethyl orthoacetate.

针对目前工业上回收3,3-二甲基-4-戊烯酸-3'-甲基-2'-丁烯酯工艺复杂、异戊烯大量分解问题,研究了MBDP直接返回循环使用工艺,MBDP与甲醇、原乙酸三甲酯或与甲醇原乙酸三甲酯混合溶液进行酯交换反应的回收新工艺,比较了四异丙基钛酸酯、甲醇钠、氢氧化钾用作酯交换催化剂的效果,筛选出回收MBDP&一锅煮&最佳工艺:按照n:n:n:n=1.0:9.8:9.2:0.05物质的量比投料,74℃~78℃反应21h,MBDP转化为贲亭酸甲酯和异戊烯醇,单程转化率87.7%,用85%的磷酸中和甲醇钠,蒸出甲醇,直接用于合成贲亭酸甲酯。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

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Several theories about reigniting and extinguishing of the arc have been refered.

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