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Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖,琼脂0.7%,pH5.8 培养基中25℃暗培养20d 后继代一次,诱导产生Ⅱ型胚性愈伤组织,用镊子将其夹碎成2mm2大小的小块,置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中,侵染时间为45min,共培养3 天后,转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天,再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中,遮光培养20d后,置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化,卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%;同时以甘蔗心叶愈伤组织材料,通过循环水式真空泵,产生负压,设定不同的负压值,在农杆菌菌液中感染5min 后,在负压条件下继续侵染15min 后,筛选出抗性愈伤组织并获得转化植株,其中在负压为-0.05 时转化率达到最大值,其卡那霉素抗性愈伤组织获得率平均为37.5%。

In chapterⅡ, considering GLMMs data which the number of high units is different and the number of low units is small and unbalanced, we Conduct a series of simulations, the simulations show that regardless of the number of the level 2 units,the Bayesian estimate of random effects residual variance is more precise than PQL and the PQL estimation deviates from the true value seriously; In terms of the fixed effects estimates, When the number of level 2 units is 20, The mean and median of the Bayesian estimate is more precise than PQL,But With the number of level 2 units increase,the estimate of two methods is similar. Thus, in practical applications, we recommend the use of Bayesian method.

第二章针对不同高水平单位数,低水平数较小且不平衡的GLMMs数据,进行模拟研究,研究表明:无论水平2单位数的大小,贝叶斯方法随机效应残差方差估计值远比PQL法更接近真实值,PQL法严重偏离了真实值;而对固定效应参数估计值,当水平2单位数为20时,贝叶斯法各参数的均数和中位数均要较PQL法更接近真实值,而随着水平2单位数的增加,两种方法在估计固定效应参数时相差并不大,因而,在实际应用中,推荐采用贝叶斯法。

The results showed an increased mean jitter value, fiber density and abnormal percentage both in EDC and AT muscles in group 1 and II diabetic patients comparing to the normal controls. These findings suggest an impaired or immatured neuromuscular junctions and an evidence of reinnervation through axonal sprouting in the diabetic patients either with or without nerve conduction abnormalities. In conclusion, the changes of axonal degeneration and reinnervation are the main pathophysiological mechanism of diabetic neuropathy, and the SFEMG is more sensitive than routine nerve conduction study in the diagnosis of diabetic neuropathyKeyword Axonal degeneration , diabetes mellitus , neuropathy , single fiber electromyography

结果显示两组糖尿病病患的连续间值差异,肌纤维密度及不正常比率比对照组显著增加(t试验,依序p.01),在第一组糖尿病患中,伸指总肌的连续波间值差异比第二组高(p.05),而连续波间值差异与纤维密度之间也成线性相关的增加(伸指总肌,r=0.471,p.01;胫前肌,r=0.386,p.02),在伸指总肌测得的连续波间值差异上,第一组糖尿病患有75%不正常,第二组有33.3%不正常,而在胫前肌的连续波间值差异上,第一组糖尿病患有83.3%的不正常,第二组有75%不正常,在肌纤维密度的测定中,第一组糖尿病患的伸指总肌有27.8%不正常,胫前肌有58.3%不正常,第二组病患的伸指总肌有25%不正常,胫前肌有41.7%不正常,此结果表示糖尿病神经变造成的神经肌像交接处不稳定及有神经轴再生的重分布现象,因此也证实糖尿病神经病变的主要病理机转为神经的退化所致,而在诊断上单纤维肌电图检查的敏感度也比运动神经传导速度检查为高。

The results from the experiment are: the amount of all micro-powders that grain sizes are less 0.044 mm should be less than 35% in weight, and the value of coarse q and attenuate q should be respectively 0.23 and 0.32, and the additive amount of active a-alumina and calcium alumina cement should be respectively 7% and 4% in weight, meanwhile, the best dispersant is PMAA-NH4 in the non-microsilica system; while the value of two q should be respectively 0.21 and 0.29, the amount of active a-alumina, microsilica and calcium alumina cement should be respectively 6%, 2% and 4% in weight, the best dispersants are sodium hexamer taphosphate and Na2P3Oio in the microsilica system.

依照上述实验方法得出刚玉自流浇注料所必备的条件:刚玉自流浇注料中的细粉(-0.044mm)总量应小于35%;在无硅微粉系统中的Andreassen公式的粗端q值和细端q值分别为0.23和0.32,氧化铝微粉、水泥的最佳加入量是7%、4%,且所使用的分散剂为改性聚丙烯酸钠最好,而在含硅微粉系统中的Andreassen公式的粗端q值和细端q值分别为0.21和0.29,氧化铝微粉、硅微粉和水泥的最佳加入量为6%、2%和4%,最好合用三聚磷酸钠和六偏磷酸钠作为分散剂。

To study the function of thyroid hormone receptors in osteoblasts and in bone, the nuclear extracts of TRs from human osteoblast-like cell line MG63 and rat calvaria were achieved, and the two main kinetic parameters of TRs, affinity constant and maximal binding capacity, were determined by radio-ligand binding assay and Scatchard plot.

其中MG63和骨组织中TRs的Ka值与文献报告的人类外周血淋巴细胞以及大鼠脑组织和肝脏中TRs的Ka值相比,差别显著,但与文献中其他成骨细胞系的Ka值相近,提示成骨细胞或骨组织中的TRs与THs的结合能力有可能确实低于淋巴细胞、脑组织或肝脏,从而使得THs在骨组织中的作用机制及效果可能有别于其他组织。

Agilent 54123T | HP54123T 34GHz, 4 passageways count word oscillograph An Jielun Brand: Oscillograph of 54123T of An Jielun of HP of HP of American An Jielun Agilent is undee indication instrument, it can be accurate the weaveform that describes telegraphic number, show in wide example application signal weaveform changes as the change of time, vertical axis is worth on behalf of undee voltage, horizontal axis represents time, the adjustment of brightness says for Z axis A kind of of oscillograph of An Jielun 54123T simple weaveform shows, the certain and special event that can help you discover to appear in telegraphic date weaveform, be like: Flatter pull Mou goes straight towards of Wei of ㄐ of haze of creek rapid pull to touch Su You to be worth lotus root of green of Lu of U of Fei of bursa post left-eyed flounder the sometime that " of?" drift part produces often in circuit weaveform paragraph or some part, whether does signal produce mutation and twist, the between change when noise signal and this noise signal containing to follow in the communication part that contains in signal and dc composition and undee signal Characteristic: The Gao Zhoubo with unapproachable * is characteristic * exceeds fanciful pulse width Converter of *12 digit model The lowermost time-interval of *0.25 skin second * is perpendicular and average measure mode Parameter of * automatic pulse is expressed * 4 passageways Voltage of time of * high accuracy is worth * HP attestation * for many times the brim sparks * time domain reflexes a function * digitlization filter

Agilent 54123T | HP54123T 34GHz、4通道数字示波器安捷伦品牌:美国安捷伦Agilent 惠普HP 安捷伦54123T示波器是波形显示仪器,它能正确形容电信号的波形,在宽泛的实例应用中显示信号波形跟着时候的转变而转变,垂直轴代表波形的电压值,程度轴暗示时候,亮度的调整称之为z轴安捷伦54123T示波器的一种简单的波形显示,能够帮助你发现电信号波形中呈现的某些特别事务,如:在某个时候和某个波形幅值之间呈现的一个异常震动信号,%26rdquo;漂移部门%26rdquo;相对频繁地发生在电路波形中的某一时候段或某一部门,信号是否发生变异和扭曲,信号中所含的交流成分和直流成分以及波形信号中所含噪声信号以及该噪声信号随时候的转变特点:*无与伦比的高周波特征*超乎想像的脉冲宽度*12位数模转换器*0.25皮秒的最低时候距离*垂直平均测量模式*主动脉冲参数表*四个通道*高精度时候电压值*惠普认证*屡次的边缘触发*时域反射性能*数字化滤波器

First, we introduce and discuss the various methods of multivariate polynomial interpolation in the literature. Based on this study, we state multivariate Lagrange interpolation over again from algebraic geometry viewpoint:Given different interpolation nodes A1,A2 .....,An in the affine n-dimensional space Kn, and accordingly function values fi(i = 1,..., m), the question is how to find a polynomial p K[x1, x2,...,xn] satisfying the interpolation conditions:where X=(x1,X2,....,xn). Similarly with univariate problem, we have provedTheorem If the monomial ordering is given, a minimal ordering polynomial satisfying conditions (1) is uniquely exsisted.Such a polynomial can be computed by the Lagrange-Hermite interpolation algorithm introduced in chapter 2. Another statement for Lagrange interpolation problem is:Given monomials 1 ,2 ,.....,m from low degree to high one with respect to the ordering, some arbitrary values fi(i= 1,..., m), find a polynomial p, such thatIf there uniquely exists such an interpolation polynomial p{X, the interpolation problem is called properly posed.

文中首先对现有的多元多项式插值方法作了一个介绍和评述,在此基础上我们从代数几何观点重新讨论了多元Lagrange插值问题:给定n维仿射空间K~n中两两互异的点A_1,A_2,…,A_m,在结点A_i处给定函数值f_i(i=1,…,m),构造多项式p∈K[X_1,X_2,…,X_n],满足Lagrange插值条件:p=f_i,i=1,…,m (1)其中X=(X_1,X_2,…,X_n),与一元情形相似地,本文证明了定理满足插值条件(1)的多项式存在,并且按&序&最低的多项式是唯一的,上述多项式可利用第二章介绍的Lagrange-Hermite插值算法求出,Lagrange插值另一种描述是:按序从低到高给定单项式ω_1,ω_2,…,ω_m,对任意给定的f_1,f_2,…,f_m,构造多项式p,满足插值条件:p=sum from i=1 to m=Ai=f_i,i=1,…,m (2)如果插值多项式p存在且唯一,则称插值问题适定。

Where is a partial loss of goods, merchandise, or other moveables, the measure of indemnity, subject to any express provision in the policy, is as follows:(1)Where part of the goods, merchandise or other moveables insured by a valued policy is totally lost, the measure of indemnity is such proportion of the sum fixed by the policy as the insurable value of the part lost bears to the insurable value of the whole, ascertained as in the case of an unvalued policy:(2) Where part of the goods, merchandise, or other moveables insured by an unvalued policy is totally lost, the measure of indemnity is the insurable value of the part lost, ascertained as in case of total loss:(3) Where the whole or any part of the goods or merchandise insured has been delivered damaged at its destination, the measure of indemnity is such proportion of the sum fixed by the policy in the case of the valued policy, or of the insurable value in the case of an unvalued policy, as the difference between the gross sound and damaged values at the place of arrival bears to the gross sound value:(4)"Gross value" means the wholesale price, or, if there be no such price, the estimated value, with, in either case, freight, landing charges, and duty paid beforehand; provided that, in the case of goods or merchandise customarily sold in bond, the bonded price is deemed to be the gross value.

货物、商品或者其他动产发生部分损失的,除保险单另有明文规定外,赔偿范围如下:(1)如果定值保险单承保的货物、商品或者其他动产的一部分发生全损,赔偿范围为保险单确定的保险金额中的比例部分,该比例为灭失部分的可保价值占全部可保价值的比例部分。可保价值的确定与不定值保险相同;(3)若不定值保险单承保的货物、商品或者其他动产的一部分发生全损,赔偿范围为损失部分的可保价值,可保价值的确定与全损时相同;(4)如果被保险货物或商品的全部或者一部分以受损状态运抵目的地,赔偿范围以完好毛值与受损价值的差额占完好毛值的比例计算,在定值保险单情况下,是保险单确定的金额的该比例部分,或者,在不定值保险单情况下,是可保价值的该比例部分;(5)&毛值&是指货物的批发价格,如果没有这种价格,则是货物的估计价值,不论哪一种情况,再加上已预先付讫的运费、卸货费和关税之后的价值;但是,如果货物或商品按惯例在关仓出售,那么关仓价格就视为毛值。

The dissertation recommends some kinds of methods and measures: such as adjusting the network configuration, connecting value and threshold value by the total value of all stylebooks, adding part of the adjusting value of the last time to the current adjusting value, transforming the stylebooks to standard value, optimizing activation function, appending threshold value to the putout of the nerve cell and so on. Simultaneity, the author brings forward a new method to optimize the model of artificial neural network . It is using the automatically adaptive genetic algorithm to make the network configuration, connecting value and threshold value of artificial neural network better. The method can make the model better and improve the simulating effect and forecasting precision.Genetic algorithm is an arithmetic based on evolution and genetics used to search the optimization .

本文针对人工神经网络应用中存在的上述问题,介绍了各种改进方法与措施:如用所有样本的总效果对网络权值矩阵和阈值向量进行调整、调整量中加入动量项、标准化训练样本数据、优化激励函数以及给神经元的输出值添加偏置量等;同时还提出了一种新的优化人工神经网络模型的方法,即采用自适应遗传算法对人工神经网络模型的网络结构和权值阈值进行全局优化搜索,以提高大坝安全监测人工神经网络模型的拟合成果和预测精度。

Proposing simple evaluation methods and evaluation values are the key factors affecting the popularizing of self-compacting concrete technology. The advantage and disadvantage of several methods for evaluating the workability of self-compacting pumpcrete, such as slump, bleeding under pressure, slump flow, the outpour time of inverse slump cone, the flow speed of Orimet meter,...

探讨了坍落度、压力泌水、扩展值、中边差、倒坍落筒流下时间、Orimet仪的流出速度、L型流动实验等多种评价免振捣泵送混凝土工作性能的指标及其优缺点;经对比分析并结合实验研究和工程实际,提出了实用的新拌免振捣混凝土工作性评价指标及其合理取值区间:坍落度一般应控制在 230~260mm,倒坍流下时间 8~15s,扩展值 600~700mm,中边差值≤25mm,压力泌水实验中的S10不超过 40%, V140在 40~110mL。

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推荐网络例句

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。