中

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

The result of factor analysis can provide the changes of relative precipitation and temperature variations. In trace elements, Cu, As, Zn, Pb and Ni were apparently enrichmented in soil layers and are sensitive to climatic changes. Carbon isotope is more sensitive to climatic changes than that of oxygen isotope in loess stratigraphy. The heavy mineral are varified in the loess stratigraphy, but their content differences between loess and palaeosol is little. Though the palynologic analyses results reveal that pollens and spores are fewer in the loess stratigraphy, they differ greatly in loess layer and palaeosol layer not only in species but also in types, i. e., the pollens and spores indicating warm and moist climate, such as Lles, Podocarpus, Polopydium, Pteris grerilliana Wall, Pterocarya and Juglans appear in palaeosol layers, while those indicating cold and dry climate such as Ephedra, Artemisia, Chinopodiaceae, Picea and Abies exist mainly in loess layers. The pollen content of broad-leaved tree mainly consisting of Pterocarya is up to 60% in palaeosol 〓, while the content of Ephedra is more than 50% in 〓 loess layer, showing that the environment differed greatly when 〓 and 〓 were formed.

当然，常量元素氧化物的组合因子分析结果可提供相对降水与相对温度在剖面的变化，因而来反映气候状况更为理想；微量元素中Cu、As、Zn、Pb与Ni在成土过程中富集明显，亦较好地反映了气候变化；稳定同位素中的碳同位素比氧同位素更能敏感记录气候的变化；研究区黄土地层中重矿物种类较多，但黄土层与古土壤层中含量差异不大；孢粉分析结果揭示，尽管研究区黄土层中孢粉总量相对较为贫乏，但黄土层与古土壤层中孢粉种属相差较大：在古土壤层内出现了指示相对暖湿环境的冬青属、罗汉松属、水龙骨属、林下凤尾蕨、枫杨、胡桃等植物孢粉，而黄土层中麻黄、藜科、蒿属及冷杉、云杉含量较高。

There in the horrors of prisons, lunatic asylums and hospitals, in drab suburban pubs, in brothels and gambling-hells, in the salons of the elegant, the Stock Exchanges, socialist meetings, churches, revivalist gatherings and ecstatic sects, through love and hate, through the experience of passion in every form in his own body, he would reap richer stores of knowledge than text-books a foot thick could give him, and he will know how to doctor the sick with a real knowledge of the human soul.

在牢狱、精神病院、医院的恐怖中，在沉闷的乡村酒馆中，在妓院与赌场中，在高雅沙龙中，在股票交易所中，在社会党人集会中，在教堂中，在信仰复兴运动者集会中，在狂热宗教流派中——借助于爱与恨、借助于对每一种自身内在激情的体验——人类心理的探索者能够收获更多的知识，远胜于厚厚的书本。从此，他学会如何使用有关人类心灵的真正知识，来医治病患者。

Of prisons, lunatic asylums and hospitals, in drab suburban pubs, inbrothels and gambling-hells, in the salons of the elegant, the StockExchanges, socialist meetings, churches, revivalist gatherings andecstatic sects,through love and hate, through the experience of passionin every form in his own body, he would reap richer stores of knowledgethan text-books a foot thick could give him, and he will know how to

在牢狱、精神病院、医院的恐怖中，在沉闷的乡村酒馆中，在妓院与赌场中，在高雅沙龙中，在股票交易所中，在社会党人集会中，在教堂中，在信仰复兴运动者集会中，在狂热宗教流派中——借助于爱与恨、借助于对每一种自身内在激情的体验——人类心理的探索者能够收获更多的知识，远胜于厚厚的书本。

Prisons, lunatic asylums and hospitals, in drab suburban pubs, inbrothels and gambling-hells, in the salons of the elegant, the StockExchanges, socialist meetings, churches, revivalist gatherings andecstatic sects,through love and hate,through the experience of passionin every form in his own body,he would reap richer stores of knowledgethan text-books a foot thick could give him, and he will know how to

在牢狱、精神病院、医院的恐怖中，在沉闷的乡村酒馆中，在妓院与赌场中，在高雅沙龙中，在股票交易所中，在社会党人集会中，在教堂中，在信仰复兴运动者集会中，在狂热宗教流派中——借助于爱与恨、借助于对每一种自身内在激情的体验——人类心理的探索者能够收获更多的知识，远胜于厚厚的书本。

Of prisons, lunatic asylums and hospitals, in drab suburbanpubs, inbrothels and gambling-hells, in the salons of the elegant, theStockExchanges, socialist meetings, churches, revivalist gatheringsandecstatic sects,through love and hate, through the experienceof passionin every form in his own body, he would reap richer stores ofknowledgethan text-books a foot thick could give him, and he will knowhow to

在牢狱、精神病院、医院的恐怖中，在沉闷的乡村酒馆中，在妓院与赌场中，在高雅沙龙中，在股票交易所中，在社会党人集会中，在教堂中，在信仰复兴运动者集会中，在狂热宗教流派中——借助于爱与恨、借助于对每一种自身内在激情的体验——人类心理的探索者能够收获更多的知识，远胜于厚厚的书本。

In the biological treatment, anaerobic biological treatment is used to reduce selenate ions and/or selenite ions presented in the raw wastewater to transfer to single insoluble selenium.

在生物法中，采用厌氧生物处理来降低原始废液中可溶的硒酸盐与亚硒酸盐，并转化为不溶性的单质硒，从而以不溶性单质硒的形式去除硒酸盐或亚硒酸盐；在絮凝沉淀法中，通常是向生物处理后的废水中加入一种金属盐，使该金属盐与可溶性硒反应并生成一种不溶性硒化合物，从而以不溶性硒化合物形式去除残留的可溶性硒；在过滤法中，将絮凝沉淀法中残留在废水中的不溶性硒去除，这样就能在不用大量化学药剂和不产生大量沉淀的情况下去除含硒废水中的可溶性硒。

In the part of empirical analysis of Chinese IPO first-day return, the difference between existing study and this study is that we use principal components analysis to extract from five factors to construct investors'sentiment index, add it to controled varibles, on which we examine the influence of offering mechanism on IPO first-day return. Moreover, we gather statistics and compare the IPO holding return for lockup period between fixed-price-class offering method and bookbuilding-class offering method, as well as price range between Chinese A-share market and Hongkong stock market, which prvide comprehensive evidence to appraise Chinese IPO bookbuilding mechanism. 2. Compare to the existing literatures which consider discount or allocation, incentive allocation and discount are considered at the same time in optimal mechanism design in this dissertation, and the offering bottom price is introduced to the price range. We investigate the relationship between price range and allocation quantity, and obtain the optimal price range and allocation strategy so that the advantages of independent allocation are reflected. On the other hand, the disadvantages of independent allocation are reflected in the manipulation in case of no restriction on IPO allocation. To distinguish from the existing literatures, bookbuilding mechanism is introduced to the model. We study the allocation strategy adopted by the underwriter in the pooling equilibrium of manipulation. In addition, we analyze the incentive compatibility conditions to which the existence of pooling equilibrium should satisfy and examine some factors'impact on the existence of manipulation.3. In bookbuilding without independent IPO allocation, Chinese realistic IPO background is considered and the condition of bookbuilding and fixed-price hybrid offering is added. Divide bookbuilding and fixed-price hybrid offering into sequential hybrid and simultaneous hybrid, and point out that the key points of subscription strategy in the two kinds of hybrid are different. Through modeling and simulation we obtain the impact of institutional investors'subscription strategy on bookbuilding without independent IPO allocation, which fill the gap in related research. Moreover, in the part of IPO bookbuilding with over-allotment option, the pricing strategy is divided into hot-IPO strategy and weak-IPO strategy. Take into account the procedure of Chinese IPO with over-allotment option, it presents how the underwriter determines the pricing strategy and what effect it brings to offering price and issue size through modeling and simulation. Therefore, it is instructive as a complement to existing literatures.

此外，对我国A股市场询价发行方式与固定价格发行方式下的IPO锁定期到期时持有收益率、以及我国A股市场与香港市场的询价发行价格区间进行了统计比较，为评价我国IPO市场的询价发行提供了比较全面的依据。2、相比已有文献仅考虑折价和分配中的一种激励措施，本论文在有自主配售权下的机制设计中并用了分配与折价两种激励措施，并且在价格区间的制定中引入发行底价，考察了价格区间与分配量之间的关系，得到最优的价格区间和分配策略，从中体现自主分配权的优点；在有自主分配权下的操纵行为研究中则体现了自主配售权的弊端，与已有文献不同的是，在模型中引入了累计投标询价机制，研究了承销商在混同均衡操纵中采取的分配策略，并对操纵存在的条件进行分析讨论，得出各个因素对混同均衡操纵存在的影响。3、在无自主分配权下的询价发行中，充分考虑了我国实际应用的背景，加入了与固定价格发售混合的条件，并将询价与固定价格混合发行分为序贯与同步两种方式，指出机构投资者在两种情况下申购策略的侧重点各有不同，通过对序贯混合发行方式下的网下累计投标策略以及同步混合发行方式下的资金分配策略的模型建立和数值仿真，考察了机构投资者申购策略对无自主分配权下的询价发行的影响，填补了相关研究文献的空白；在包含超额配售选择权的询价发行中，将发行定价策略分为热销策略和弱销策略，在考虑了我国含超额配售选择权的发行流程下，通过建模以及数值仿真得出承销商会对发行定价策略作出怎样的选取，进而会对新股发行价格、发售数量产生怎样的影响，对目前相关研究的缺乏做出了有益的补充。

I don't see what anyone can see, in anyone else But you I will find my nitch in your car With my mp3 DVD rumple-packed guitar I don't see what anyone can see, in anyone else But you Du du du du du du dudu Du du du du du du dudu Du du du du du du dudu du Up up down down left right left right B A start Just because we use cheats doesn't mean we're not smart I don't see what anyone can see, in anyone else But you You are always trying to keep it real I'm in love with how you feel I don't see what anyone can see, in anyone else But you We both have shiny happy fits of rage You want more fans, I want more stage I don't see what anyone can see, in anyone else But you Don Quixote was a steel driving man My name is Adam I'm your biggest fan I don't see what anyone can see, in anyone else But you Squinched up your face and did a dance You shook a little turd out of the bottom of your pants I don't see what anyone can see, in anyone else But you Du du du du du du dudu Du du du du du du dudu Du du du du du du dudu du But you

我不搞清任何人所能在其他任何人但是你中注意到的我将发现我的nitch在你的小汽车中我不和我的mp3 DVD皱纹-挤满的吉他有关搞清任何人所能在其他任何人但是你中注意到的 Du dudu dudu dududu Dudu dudu dudu duduDu dudu dudu dududu du 是沿着沿着顺而下顺左边权利而下仅仅因为我们使用骗人的东西，左边正确B一出发不意味着我们是不我不在中搞清任何人所能注意到的刺痛其他任何人但是你你是总是设法使它保持真正我是爱你怎样感到我不搞清任何人所能在其他任何人但是你中注意到的我们在两方面同样地富人暴怒的你更多想要的有光泽愉快合身扇，我想要更多舞台我不搞清任何人所能在其他任何人但是你中注意到的&堂吉诃德&是一钢是驱动男人我的名字是亚当我你的我不看见的最大扇子是在任何人所能看见其他任何人但是你的中沿着你的脸扭歪有一点和跳你摇动的舞粪块从出来你的裤子的底部我不搞清任何人所能在其他任何人但是你中注意到的 Du dudu dudu dududu Dudu dudu dudu duduDu dudu dudu dududu du但是你

The studies was proceeded on the the supercritical CO2 extraction technique of rotenone from Tephrosia vogelii. The extraction influences of technology conditions were systematically groped, The research results were listed as follows:1 The analysis method applied in this paper has been proved to be suitable to technology research of the studies on the SC-CO: extraction technique of rotenone from Tephrosia vogelii. 3 indexes, extraction ratio of rotenone. extraction ratio of total compounds and rotenone content percent in total compounds weight, were carried out with HPLC quantitatively determination combined with the sample weight loss after extraction. With these 3 indexes. The extraction influences of technology conditions were evaluated by synthesis. Then the best technology conditions were ascertained for different extraction aims.2 The pretreatment of rough material should be needed before extraction. Comminuting the material to 0.28mm size is most fit to SC-CO: extraction.3 Firstly static extracting 20 min then turn to dynamic doing is the better extraction mode than other ones.4 The orthogonal experiment was conduct with 3 factors and 5 levers.

本论文通过对非洲山毛豆SC-CO_2萃取中影响萃取效果的工艺条件的研究，初步得出如下结果：(1)本论文中所采用的萃取效果评价方法适宜于非洲山毛豆中鱼藤酮的超临界流体萃取技术研究，该方法具体为：HPLC定量分析非洲山毛豆SC-CO_2萃取物中的鱼藤酮含量，再结合萃取前后处理样品的重量损失，得出鱼藤酮萃取率、萃取所得粗物质萃取率和粗物质中鱼藤酮含量三个参数，以此三个指标综合评价萃取条件对萃取效果的影响；(2)通过对原料粒度对萃取效果影响的研究，结果表明SC-CO_2萃取非洲山毛豆复叶中的鱼藤酮，原料粉碎成30目粒度叶粉时萃取效果较好；(3)通过对萃取方式与萃取效果之间关系的研究，结果表明SC-CO_2萃取非洲山毛豆中鱼藤酮应采用先静态后动态的萃取方式，静态萃取时间为20min；(4)通过温度、压力和CO_2流量三因素五水平的正交试验，结果表明温度是最主要的影响因素，压力因素对鱼藤酮萃取率的影响程度高于CO_2流量，而对于粗物质萃取率和粗物质中鱼藤酮含量CO_2流量因素高于压力。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。