中

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

In degraded PSM matching classification, errors modification method of degraded PSM is presented and the matching tensor of every canonical scattering centers is given. In Chapter 4, the theory of wideband millimeter-wave radar target identification is introduced. It is indicated that, for HR radar target identification, description of temoral relations among features and pattern recogntion adaptive to variation of target angles are of key importance. A rule-based pattern recogniton method of sequential reasoning is proposed, which uses a series of rules to describe relations of features variation caused by target angles variation and has the advantages of adaption to unrandom variation and false probability control in classification over traditional statistical pattern recognitiop method. Feature extraction is crucial step in target identification. In range profiles identification application, features are extracted by means of range domain pre-processing algorithm, spacial and amplitude visual computation directly from range profiles and transform algorithm based on range profiles. Visual or transformed features are either sufficiently convinced or necessarily convinced and both of them are effective and robust to range profiles identification.

在第四章，首先对宽带毫米波体制背景下目标识别方法的一些特点进行了阐述并指出，在毫米波雷达目标识别中，特征之间动态关系的描述以及能适应目标姿态角变化的模式识别方法的研究乃是要解决的关键问题；进而提出了一种基于规则库的序贯推理模式识别新方法，在这种方法的研究中，主要包括序贯推理规则库的规则排列与特征选用顺序以及规则库的收敛等问题；这种方法克服了传统的统计模式识别方法特征利用效率不高、难以适应特征值的非随机性变化的缺点，把姿态角变化所引起的特征的变化用一系列规则加以表示，其优点是能适应特征值的非随机性动态变化，并能控制分类过程中的差错概率α；特征抽取是目标识别中的关键步骤，在基于目标距离像的特征抽取方法研究中，提出了距离空间域的预处理算法、距离空间域与幅度域的直观特征抽取方法以及基于目标距离像变换分析的特征抽取方法。

In groups A and B, 231 of 352 patients (66%) and 256 of 352 patients (72%) achieved an end of treatment response, and 171 of 352 patients (49 %) and 186 of 352 patients (53 %) a sustained virological response, respectively.

两组患者人口学和基线病毒指标相似，在A组和B组中，352例中231例（66%）和352例中256例（72%）获得治疗结束时应答，352例中171例（49%）和352例中186例（53%）获得持续病毒学应答。

The geology research of carbonate reservoirs in Ordovician of Tahe oilfield showed that many large dissolved vugs and fractures were developed. The spread of the reservoir take on a very heterogeneous state. And the typical fractured-vuggy reservoirs was formed. According to the combination of the various spaces, the formation was divided into vuggy formation, fractured-vuggy formation and fractured formation. The research showed that development of the vug is relevant to the its position on the structure, the fractures, the water surface and the weathering surface. Multi-phase charge and recharge of the oil in the reservoirs resulted in the complexity of the fluid distribution. So the"Bottle Model"was brought forward to explain the movement of the water-oil contact. The special storage space and the complex fluid distribution lead to the unstability of the production and the complexity of the water cut. In order to explain the characteristic of the fracture-vug unit was brought forward and the basic principle and method of the partition of the fracture-vug unit was formed. The classification and evaluation of the units were performed according the reserve and energy. Based on the research of geology characteristic and the fluid flow in the reservoir the comprehensive numerical simulation plan of the fractured-vuggy reservoirs were established. Through the selection of the simulation unit, fluid flow type and parameter equivalent the triple media reservoir simulation model was established. Considering the practical application the model was resolved by the DKR decomposition conjugate grads method. Based on the fine reservoir description of Dsitrict IV of the Tahe oilfield the various space type were classified and combined together. The geology model of Unit S48 was constructed. Two typical single well model was established according to the geology and the dynamic phenomena. Finally the single well model and the Unit S48 were simulated by the triple media reservoir simulator. Via the local grid refinement and coarsening in the simulation good matchs were gained. Based on the results of the simulation the reserve distribution, percent of reserve produced in various space and the natural energy were analyzed. The fluid was storaged in the fractures and vugs mostly and more than 90% the produced oil came from the systems. The energy belonged to the active one. The results of simulation accorded with the fact and showed the validity and practicability the research and the simulator.

塔河油田奥陶系碳酸盐岩油藏的地质研究表明，其储层中发育着很多大型的溶蚀洞、缝，储层的平面展布呈现出极度的非均质性，形成了典型的缝洞型碳酸盐岩油藏，根据各种孔隙介质在储层中的组合，将储层分为了溶洞型、裂缝-溶洞型和裂缝型三类；研究表明溶洞储层的发育和构造位置、裂缝的发育、潜水面和风化面的位置等因素有关；多期充注的油气成藏模式导致了流体复杂的赋存状态，由此提出了所谓的&瓶子模型&，解释生产过程中油水界面的变化；特殊的储集空间类型和流体分布特征导致油田在开发过程中表现出很大的不确定性和含水变化的复杂性，为了合理的解释油气田开发过程中的动态特征，提出了&缝洞单元&的概念，并制定了&缝洞单元&纵横向划分的基本原则和依据及划分方法，并对&缝洞单元&进行了分类和评价；基于地质特征和流体在其中流动规律的研究，提出了缝洞型碳酸盐岩油藏的数值模拟综合解决方案，通过模拟单元的选择、流动类型和参数的等效，建立了三重介质油藏三维三相数值模拟模型，采用不完全LU分解预处理共轭梯度法进行了求解；在塔河油田4区精细油藏描述的基础上，将各种类型的孔隙空间进行了归类组合，建立了S48单元的地质模型；通过对油井生产动态进行分析研究，建立了两类和油井地质、生产动态相对应的单井模型；最后应用三重介质油藏数值模拟软件对单井模型和S48单元进行了数值模拟，通过局部加密和粗化等技术模拟流体流动规律，取得了很好的拟合效果；结合数值模拟结果，分析了各种介质中的储量分布、储量产出的百分比以及地层的能量，认为塔河油田缝洞型油藏中流体绝大多数储集于缝洞系统之中，所产出流体90%以上也来自于缝洞系统，其底水能量属于较充足的类型；模拟结果和油田实际情况符合较好，说明了地质研究和油藏数值模拟研究的正确性。

Brushing ABA or BA onto the upper leaves or ears of H. vulgare increased the Na+ content in the whole plant, especially in the ears, stems and sheaths, compared with that of NaC1 only treatment. The K(superscript +) content showed no significant changes in any treatments.

大麦根和冠中的Na含量均有增加的趋势，冠中Na含量增加幅度更大；冠中的Na主要是从叶片向叶鞘、茎和穗中运输积累，穗中Na的积累最显著。

The intensity ratio of TO and LO inMCT was observed to be different. Such difference was explained in terms of the different Ramangeometry arrangement.〓. The laser-induced micro-photoluminescence in the range of 1000～5000〓(1.34eV～1.83eV) was found for the first time in LPE MCT epilayer. The center of photoluminescence wasat 2750〓 or 1.62eV and the FWHM of luminescence was 2000〓 or 0.25eV. We assume thatthe photoluminescence is due to recombination of electron from an anion vacancy resonance levelto the top of valance. In addition, new Raman shift was observed at 750〓 in LPE MCTepitaxial film.〓. The laser-induced micro-photoluminescence with quasi-periodic structure was observed forthe first time at room temperature in one of MOVPE MCT epitaxial film samples. The range offluorescence was from 1.46eV to 2.21eV, i.e., 1.73eV above the conduction band edge.

2首次在LPE生长的碲镉汞外延薄膜的显微Raman谱中，在1000～5000〓范围发现了激光激发显微荧光，该荧光的发光范围换算为电子伏特标度为1.34eV～1.83eV，荧光的发光中心大约位于2750〓，即1.62eV，发光的半峰高宽约为2000〓或0.25eV；指出该显微荧光来源于碲镉汞薄膜中的阴性离子空位共振能级的激光激发发光；观察到了碲镉汞外延薄膜中一个新的Raman散射峰，位于750〓位置； 3首次在一块用MOVPE方法生长的〓Te外延薄膜的显微Raman谱中，发现了1.46eV至2.21eV范围并伴随有周期结构的显微荧光峰，该发光峰对应的能带中心位于〓Te材料导带底上方1.73eV，通过研究得出样品在1.46eV至2.21eV范围的显微荧光峰是由于改进 MOCVD 生长工艺，提高了碲镉汞外延薄膜的结构质量所致；通过分析指出该显微荧光来源于外延层中的阴性离子空位的共振能级发光。

ISO 10303, is issued by international standardization organization, and here, this paper gives the definition of product structure model oriented part41 and part44 in STEP and content of product structure management in PDM. This paper also presents method of creating and extracting product information on engineering draught and product data exchange and integration between CAD system and database manager system. Moreover, the paper gives a method of organization and manager of data base on product structure.

论文中依据国际标准化组织颁发的STEP标准即ISO 10303中的Part41，Part44两部分内容以及PDM中有关产品结构管理方面的功能含义定义了产品结构模型，实现了工程图样中产品管理信息的建立和提取以及数据库系统与图形文件中的管理信息交换与集成，并以产品结构为核心组织和管理产品结构模型数据。

While the morning-band has not beclouded my eyes While the cord of the Fates is still dimly evident In the dusk, I am busy In twining the life in dust Mosquitoes are not tired of reciting doggerels again and again I am immaculate to respectively listen to them Moths are looking for sunshine in sunshine I am not intent to bluntly point it out Time is like starlight Soundlessly trickling from reticulations I only hold the undivided attention To weave the life in dust Oh, when will the silk thread As long as a dream be ended In my bustling steps Where is the unchangeable purpose A gigantic beetle Has destroyed my dream that is as long as the silk thread What has dropped from the sky to the earth also includes The life I am weaving in dust

趁黑纱尚未蒙蔽我的眼睛昏黄中这一线命运的绳索还依稀分明，我要忙着织完这尘埃中的生活蚊子不厌其繁地朗诵打油诗我无暇聆听飞蛾在光明中寻找光明我无心点破时间如星光从网眼里无声地滴漏我只一心一意纺织这尘埃中的生活梦一样长的丝线啊何时才有个尽头在我忙乱的脚步里哪里有不可更改的目的一只巨大的甲壳虫摧毁了我丝线一样长的梦从空中坠落在地的，还有这我正在尘埃中编织的生活

For the mode of legislation, it is suggested that a principle regulations to be made while the detailed applied situation and conditions are specified further in judicial explanation; regarding the applied situation, give four kinds of examples as shortage of capital, mixing of company, property of shareholders, business and personality, avoid the liability of contract and legal liability using of company personality; when making judgment, such three parts as main body, action and results have to be considered. The voluntary creditor and forced creditor consists of prosecutor, those person who abuses the force of company is the defendant who has the act of abusing company personally resulting in bad effect; the responsibility of producing of evident may refer to those of courts of Germany, which is proved by prosecutor, after that, the defendant provides the evident that he has a proper action for the suit of company or his action is not the direct cause for the loss of company; the legal effect of disregarding of corporate personality is to deprive the rights of limited liability of shareholders and shall undertake the affiliated liability according to different situations of company debts, however, it is clear that the legal effect is only limited to the special legal relationship in the specific case; the exercising body is made by court while the corporate personality might not be disaffirmed directly in exerting process; finally, it points out the real essence and also its real sense and difficulties in practice.

对立法模式，建议成文法中只做原则性规定，而具体的适用情形、适用条件等规定可以在司法解释中进一步作出明确规定；对适用情形，根据我国实际列举了资本显著不足、公司与股东财产、业务、人格的混同、利用公司人格规避契约义务和逃避法律义务四种大类；审判时应考虑的要件包括主体、行为、结果三大要件，自愿债权人和非自愿债权人构成原告，对公司有控制力的滥用者为被告，要有滥用公司人格的行为，并有损害的结果产生；举证责任可参照德国法院的做法，由原告提供初步证据证明公司存在滥用公司人格的行为，而后，由被告提供证据证明自己与涉诉公司的关系是正当的，或自己的行为与原告的损失没有因果关系；否认公司人格的法律效果是剥夺股东在个案中的有限责任的权利，视不同情形对公司债务承担连带责任，但应明确法律效力仅限于个案中的特别法律关系；行使主体应由法院的审判庭行使，而不宜在执行程序中直接否认公司法人人格；最后对逆向揭开公司面纱作了探讨，指出其现实意义和实践难点。

In order to disclose the adaptive strategies and capacity of Potaninia mongolica Maxim, acquired during its evolution history, the whole response system, which was composed of individual morphology plasticity response, organs anatomy structure response, physiological function response of anti-arid and anti-hotness, activities of protective enzymes response, endogenous hormone adjustment response, sexual reproduction strategies response of seeding, dispreading, sprouting and renewing, asexual reproduction strategies response of clonal growth patterns, clonal growth architectures, clonal growth architectures plasticity, heterogeneous resources utilization strategies, endogenous hormone distribution in clonal organs, foraging behavior, risk-spreading, and resource sharing, individual density allocation patterns response, niche differentiation response, species connection response, allelopathy response and biodiversity components response of the plant was profoundly explored in this paper.The studies come to at least four important results:(1) Taking the sensitive response and evading strategy to adapt to environment stress, Potaninia mongolica Maxim is a successful species has lived through the long-term evolution. Nevertheless, it is narrow climate and soil niche and characteristic of vegetation reproduction which has made the plant a rare and endangered species.

本论文通过对绵刺环境胁迫下个体形态可塑性响应、器官解剖结构响应、抗旱性和抗热性生理功能响应、保护酶系统激活响应、内源激素调控响应，有性繁殖对策中结实、扩散、萌发、更新等生活史过程响应，无性繁殖对策中克隆生长格局响应、克隆生长构型响应、克隆生长构型可塑性响应、克隆生长构型与资源异质性响应、内源激素调控克隆生长构型响应、克隆器官结构与功能响应、觅食行为响应、风险分摊响应、资源共享响应，种群中格局配置响应、生态位分异响应，群落中种间关联、异株克生响应、生物多样性组成响应等内容进行研究，揭示绵刺在进化过程中的适应对策和适应能力，为绵刺保护提供理论依据。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。