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In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象,分别对文蛤幼虫发育过程贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程细胞凋亡相关的caspase三个基因进行了克隆,分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征,解析了其可能的功能,并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响,进行了目标蛋白的功能验证,详述如下:研究结果显示,在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水培养,发育成无壳的畸形,随着人工海水铁离子添加浓度的升高,幼虫长出壳状组织接近正常状态;而发育到L1期幼虫放入不含铁离子的人工海水培养却可以发育出正常的壳,推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

When a switch for recieving a waiting and a switch for an information communication are carried with a turned-on power supply, a data which represents a communication mode is drawn out in P_REV field P1,P2, came from a re-captured base station, such as ESPM, EHDM, GHDM ect., a display indicative, which represents the communication mode used by the captured base station, produced by the drawn-out data, is displayed in the PICI information display region of a LCD display.

一种移动通信终端装置,为了在信息通信过程使用户能够确认可以使用的通信方式而事前回避进行不情愿的通信,在电源被接通时、进行接收等待的切换时、以及进行信息通信的切换时,由来自重新捕捉到的基站的ESPM、EHDM或GHDM等的P_REV域P1、P2抽出表示通信方式的数据,根据此抽出的数据生成表示上述被捕捉到的基站使用的通信方式的显示标志、在LCD显示器的PICT信息显示区域显示。

Expression of p38 mitogen-activated protein kinase in chronic rhinosinusitis and its significance The expressions of p38MAPK、Phospho-p38MAPK was detected with immunohistochemistry method in uncinate press mucosa from 20 chronic rhinosinusitis patients and middle turbinate mucosa samples from 10 submucous resection patients.

p38MAPK在慢性鼻-鼻窦炎黏膜组织的表达和意义的研究应用免疫组化方法检测MAPK家族成员p38(p38MAPK)及其活化(Phospho-p38MAPK)形式在20份慢性鼻-鼻窦炎钩突黏膜标本的表达,以鼻隔偏曲的鼻道侧鼻甲黏膜为对照。

During a total of 114 339 person-years of follow-up, 356 persons caught stroke (240 cases ischemic, 97 cases hemorrhagic and 19 cases unclassified).

随访114 339人年,共发生脑卒事件356例,其缺血性卒240例,出血性卒97例,未分类卒19例,总脑卒的发病率为311.4/10万人年。

In view of this point, Lu Ling's novels are of value, because strong intellectual complex is embedded in his novels describing either workers or peasants, in which the character's spiritual dynamics receives utmost concernViewing from the perspective of the intellectual quality-freedom, enlightenment and reflection, this dissertation seizes up the traits of intellectual character of Lu Ling's novels in 1940's, in an attempt to reproduce the sheen of his novels, and also to take a glimpse at the psychological journeys of the youth generation at that ageThose characters in Lu Ling's novels behave unbridledly all the way through their life journeyo They rebel against all the absurd things, declare war on tradition, family and newly rising authority, also anatomize themselves They resort nowhere Their life is closely linked to tramp It is right during the course of tramp and rebellion that they realize themselves, look for freedom and luminosity, and achieve real contact with people; they have enjoyed the unrestraint to their heart's content as well as experience torture from various forces in heartLu Ling's novels are affluent in connotation This dissertation only studies from the perspective of intellectual, the topics among which like: freedom, tradition, individual and collective are still worthwhile studying up to now.

从这一点出发,路翎的小说很有价值。他的小说不管是描写工人还是农民,都带有浓重的知识分子情结。他的关于知识分子的小说,更是极力关注人物的精神动态。本文从现代知识分子品质的角度——自由、启蒙与反思,打量路翎四十年代小说的知识分子人物的特质,企图在这种论说使路翎小说的光彩重现,也从窥视那一代青年在那个时代的心路历程。他小说的那些人物,在人生的道路上一路狂飙过去。他们反叛一切不合理的东西,对传统、家庭、新生的权威开战,也对自己身上旧的、暧昧的因子毫不留情。他们在哪里也停留不下,他们的人生和流浪紧密地联系在一起。在这个不断的流浪和叛离,他们认识自己,追寻自由、光明,力求与人民的真正接触。也正是在这个背叛—寻找的过程,他们享受了狂飙的酣畅淋漓,也体会到了内心各种力量撕裂的痛苦。路翎小说的内涵很丰富,本文只是抽取了知识分子这个角度进行研究。当的一些命题:自由、传统、个人与集体在今天还是很有研究价值的。

The alloy catalyst was found to be unstable in the acidic solution because of the electrochemical and chemical dissolution of the catalyst.

发现在酸性溶液,钯铁合金催化剂对氧还原反应的催化活性不稳定,原因是钯铁合金催化剂在酸性溶液发生电化学/化学溶解;在碱性溶液,覆盖在电极表面的钯铁合金催化剂的质量及电化学活性面积在电化学扫描过程不发生明显变化,保持对氧还原反应的催化活性,证明钯铁合金催化剂在碱性介质非常稳定。

The above method can be used as a general method of determining molecular weight distribution of polymers.4.Solid-state ~1H wide-line NMR and ~(31)p magic angle spinningNMR were applied to a series of PEI/DNA complex samples with different PEI molecular weights and nitrogen/phosphatemolar ratios,in order to disclose the influence of various structural factors on the interactions and morphology.The results of ~1H NMR spectra showed that the linewidth of the PEI signal was greatly broadened with increasing the N/P ratio.Meanwhile,in the ~(31)p M_AS experiment,the signal was found to shift upfield with the increase of the N/P ratio.Both ~1H and ~(31)P NMR experiments demonstrated that the interaction between two constituents and subsequently the morphology of the complexes vary with the N/P ratio.

首次将固体核磁共振应用到聚合物/DNA复合物体系,观察复合物体系不同N/P比对其相互作用和相结构的影响,研究表明N/P比的变化对聚乙烯亚胺/DNA基因递送体系分子水平上相互作用有着很大的影响;另外,还发现不同分子量的聚乙烯亚胺聚合物载体在N/P比为3的情况下对复合物DNA的微观尺度没有明显影响;通过氢的自旋扩散实验,首次测得在PEI/DNA复合物体系DNA相尺寸的大小,并发现在体系N/P比达到3以上的复合物DNA和PEI达到了分子水平上的相容。

That, however, is what has happened: from the trunk of that tree of vengefulness and hatred, Jewish hatred—the profoundest and sublimest kind of hatred, capable of creating ideals and reversing values, the like of which has never existed on earth before—there grew something equally incomparable, a new love, the profoundest and sublimest kind of love—and from what other trunk could it have grown?

可这是个大事:从那报复的树干,从那犹太的仇恨,从那地球上从未有过的最深刻、最极端的、能创造理想、转变价值的仇恨生长出某种同样无与伦比的东西,一种新的爱,各种爱最深刻最极端的一种:——从其它哪根树干能够长出这种爱?

In contemporary Chinese, the combination of Noun and Verb can constitute three different structures:"NV"nominal modifying structure,"NV"verbal modifying structure,"NV"subject-predicate structure. This structure is worthy of researching because the location of it's internal component's is dispute, that is to say, whether the headword of nounbial modifier structure is verb, and whether the headword of verbal modifying structure can be noun are disputable problems.

现代汉语,名词和动词的组合可以构成三类结构:定偏正结构、状偏正结构、主谓结构,由于其内部成分的定位存在着值得争议的地方,即定偏正结构的心词是否为动词;状偏正结构的修饰语能否为名词,且容易出现同形异构结构,所以这类结构值得研究。

The geology research of carbonate reservoirs in Ordovician of Tahe oilfield showed that many large dissolved vugs and fractures were developed. The spread of the reservoir take on a very heterogeneous state. And the typical fractured-vuggy reservoirs was formed. According to the combination of the various spaces, the formation was divided into vuggy formation, fractured-vuggy formation and fractured formation. The research showed that development of the vug is relevant to the its position on the structure, the fractures, the water surface and the weathering surface. Multi-phase charge and recharge of the oil in the reservoirs resulted in the complexity of the fluid distribution. So the"Bottle Model"was brought forward to explain the movement of the water-oil contact. The special storage space and the complex fluid distribution lead to the unstability of the production and the complexity of the water cut. In order to explain the characteristic of the fracture-vug unit was brought forward and the basic principle and method of the partition of the fracture-vug unit was formed. The classification and evaluation of the units were performed according the reserve and energy. Based on the research of geology characteristic and the fluid flow in the reservoir the comprehensive numerical simulation plan of the fractured-vuggy reservoirs were established. Through the selection of the simulation unit, fluid flow type and parameter equivalent the triple media reservoir simulation model was established. Considering the practical application the model was resolved by the DKR decomposition conjugate grads method. Based on the fine reservoir description of Dsitrict IV of the Tahe oilfield the various space type were classified and combined together. The geology model of Unit S48 was constructed. Two typical single well model was established according to the geology and the dynamic phenomena. Finally the single well model and the Unit S48 were simulated by the triple media reservoir simulator. Via the local grid refinement and coarsening in the simulation good matchs were gained. Based on the results of the simulation the reserve distribution, percent of reserve produced in various space and the natural energy were analyzed. The fluid was storaged in the fractures and vugs mostly and more than 90% the produced oil came from the systems. The energy belonged to the active one. The results of simulation accorded with the fact and showed the validity and practicability the research and the simulator.

塔河油田奥陶系碳酸盐岩油藏的地质研究表明,其储层发育着很多大型的溶蚀洞、缝,储层的平面展布呈现出极度的非均质性,形成了典型的缝洞型碳酸盐岩油藏,根据各种孔隙介质在储层的组合,将储层分为了溶洞型、裂缝-溶洞型和裂缝型三类;研究表明溶洞储层的发育和构造位置、裂缝的发育、潜水面和风化面的位置等因素有关;多期充注的油气成藏模式导致了流体复杂的赋存状态,由此提出了所谓的"瓶子模型",解释生产过程油水界面的变化;特殊的储集空间类型和流体分布特征导致油田在开发过程表现出很大的不确定性和含水变化的复杂性,为了合理的解释油气田开发过程的动态特征,提出了"缝洞单元"的概念,并制定了"缝洞单元"纵横向划分的基本原则和依据及划分方法,并对"缝洞单元"进行了分类和评价;基于地质特征和流体在其流动规律的研究,提出了缝洞型碳酸盐岩油藏的数值模拟综合解决方案,通过模拟单元的选择、流动类型和参数的等效,建立了三重介质油藏三维三相数值模拟模型,采用不完全LU分解预处理共轭梯度法进行了求解;在塔河油田4区精细油藏描述的基础上,将各种类型的孔隙空间进行了归类组合,建立了S48单元的地质模型;通过对油井生产动态进行分析研究,建立了两类和油井地质、生产动态相对应的单井模型;最后应用三重介质油藏数值模拟软件对单井模型和S48单元进行了数值模拟,通过局部加密和粗化等技术模拟流体流动规律,取得了很好的拟合效果;结合数值模拟结果,分析了各种介质的储量分布、储量产出的百分比以及地层的能量,认为塔河油田缝洞型油藏流体绝大多数储集于缝洞系统之,所产出流体90%以上也来自于缝洞系统,其底水能量属于较充足的类型;模拟结果和油田实际情况符合较好,说明了地质研究和油藏数值模拟研究的正确性。

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推荐网络例句

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。