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In this study, the genomic structure and regulatory elements of ApoD from 10 representive species including protozoan, invertebrate, protochordate and vertebrate were analyzed and compared. The genomic structure of ApoD is less conserved in organisms from protostome to deuterostome invertebrates, while it is highly conserved among chordates including amphioxus and verebrates. All four conserved cysteine residues are present in amino acid sequence of deuterostome ApoDs, while there are only two cysteine residues in amino acid sequence of protostomes ApoD. Structure divergence between protostome and deuterostome ApoD proteins suggests their function difference. The majority of regulatory elements are present in nearly all organism ApoD genes ranging from unicellular protozoan to mammals, suggesting that ApoD plays a very fundamental role, and possesses a conserved regulatory mechanism. However, there also exist some specific regulatory elements, which are present only in certain species and may perform some special roles.ApoD mRNA expression in murine NIH/3T3 fibroblasts exposed to various stresses such as as hydrogen peroxide and UV light shows dose-dependent increase. And a fly homolog of ApoD, Glial Lazarillo, whose overexpression results in increased resistance to hyperoxia as well as a extension of lifespan under normoxia and resistance to starvation without altering lipid or protein content.

本文首先从生物信息学角度对分属于原生动物、无脊椎动物、头索动物和脊椎动物类群的10种动物ApoD的基因结构及调控区的调控元件进行分析及比较,发现:(1)ApoD基因外显子-内含子结构从原生动物草履虫到原口动物再到后口动物海胆的进化过程中不保守,但在分析的几种脊椎动物中相当保守;(2)文昌鱼ApoD基因扮演从无脊椎动物到脊椎动物承上启下的角色,可能代表了脊椎动物ApoD基因原型;(3)四个半胱氨酸保守位点在后口动物中都存在,而在原口动物中只存在两个,原口、后口动物ApoD蛋白一级结构上的差异反映蛋白功能上可能也存在一定差别;(4)调控区大多数主要调控元件为不同动物共有,说明ApoD主要功能及其表达调控在进化中相当保守;(5)ApoD基因个别调控元件是随着物种进化而出现并开始发挥相关作用,如SF-1;还有一些调控元件在进化过程中还没有发现其规律,这说明ApoD某些功能和基因表达调控模式可能因物种不同而存在一定的差异。

Materials and Methods: This study purposively selects patients with weight reduction experience for more than three methods as subjects from the Patient Questionnaires of Weight control Clinic NCKU Hospital. The author records the experiences of these patients from in-depth interview and focus group discussion with them. By way of content analysis, the author then compares their before/after experiences on several aspects.

本研究自成功大学附设医院家医科体重控制门诊建立的个人资料问卷中,挑选出拥有非医疗场域经验的持续减重者,进行个别深度访谈及焦点团体讨论,纪录这些持续减重者在不同减重场域的经验,再透过对这些经验的分析与诠释,比较两个场域在几个重要面向上的特点及差异。

Risk and maneuverability analysis on engineering design is widely used to conduct security appraisal abroad. The potential risks might be caused by technical parameter deviation, failure and faulty operation of facilities and pipelines in the system were normally analyzed along with the reason why deviation and faulty operation occurred. The acceptable risk was then appraised according to the results, reasons and designed protective facilities. Later, the security countermeasures and solutions were brought forward purposively to further modify and refurbish the security design and operation management.

国外广泛应用危险与可操作性分析对工程设计进行安全评价,分析系统中装置、设备、管道的个别部分工艺参数偏离、故障或误操作引起的潜在危险,分析偏离、故障出现的原因,根据后果、原因和已设计的保护装置,评估可接受的风险,并有针对性地提出安全对策和措施,用以指导修改、完善工程的安全设计和操作管理。

Apparently the concentration of Phe was higher than other compounds in emission and Nap and Pyr were usually in the majority in the remained compounds.

在排放个别PAHs成份中,其Phe浓度值明显比其他成份高出很多;在其他成份中,通常以Nap、Pyr的浓度较高。

This non-specific antigen detects reagin , an IgM or IgG immunoglobulin present in the plasma or serum of patients with syphilis and occasionally present in the serum or plasma of individuals with other acute or chronic conditions. If reagin is present it binds to the cardiolipin antigen resulting in flocculation.

个别病人应用同一种试验方法做连续的血清学检测,最好在同一个实验室进行,VDRL和RPR都是同样有效的方法,但是,由于RPR的滴度常常比VDRL的滴度略微偏高,两种方法的定量结果不能直接比较。

The conformation of the authorization system during the reform era is all-round authorization, inclined authorization, exceptional authorization, and selective recentralization of power.

改革时期的授权体制表现为全面授权、倾斜授权、个别授权、选择性集权等形态。

This tendency is manifest in certain individuals who, since the redesignation of the Red Army, have become unwilling to submit strictly to Communist Party leadership, have developed individualistic heroism, taken pride in being given appointments by the Kuomintang (i.e., in becoming officials), and so forth.

这种倾向,表现在红军改编后某些个别分子不愿意严格地接受共产党的领导、发展个人英雄主义、以受国民党委任为荣耀等等现象上面。

We recommend public transportation. Please double-check the bus numbers; Beijing has underwent quite a dramatic bus numbering reshift in the past year.

建议您使用公共交通前至会场,请留意北京最近对于公共汽车进行号码改编与个别路线撤消。

Roomless elevator at this stage of the utilization rate is very low, only emerging in individual cities, the city has developed the special requirements of the buildings are more common.

在这个阶段的使用率无机房电梯是很低,只有在个别城市出现,全市已开发出建筑物的特殊要求更为常见。

It is also possible, that some of the individual parts can be rotated, and the rest of them cannot.

这亦是可能的,这部分的个别部分可以旋转,其余的,他们不能。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。